All chemical reagents were of analytical grade. Apocynin (Acetovanillone) was purchased from Sigma-Aldrich, MO, USA; purity >98%. Complete Protease Inhibitor Cocktail tablets, EDTA free, were purchased from Roche Diagnostics, GmbH, Mannheim, Germany. All other chemicals were purchased from Sigma-Aldrich, MO, USA, unless otherwise stated.
Apocynin acetovanillone
Manufactured by Merck Group
Sourced in United States
Apocynin (Acetovanillone) is a chemical compound that can be used as a laboratory reagent. It is a naturally occurring substance found in certain plants. Apocynin has been studied for its potential biological activities, but its core function is as a research tool for laboratory experiments.
Automatically generated - may contain errors
Lab products found in correlation
Complete protease inhibitor cocktail tablet, by Roche (1 mentions)
Pdgf bb, by Cell Signaling Technology (1 mentions)
Ebselen, by Merck Group (1 mentions)
Peg catalase, by Merck Group (1 mentions)
Antibody to vinculin, by Merck Group (1 mentions)
Epidermal growth factor (egf), by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
3 protocols using apocynin acetovanillone
1
Apocynin Extraction and Purification
2
Oxidative Stress Signaling Pathways
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Apocynin (acetovanillone), PEG-catalase and ebselen were purchased from Sigma (USA). PDGF-BB and EGF were from Cell Signaling (USA). DPI, U0126, LY294002 were from Calbiochem (Germany). The primary antibodies to total Akt, phospho-Akt (Ser-473), phospho-Erk1/2 (Thr202/Tyr204), total Erk1/2, and secondary antibody were from Cell Signaling (USA). Antibody to Nox4, Duox1 and Duox 2 were from Novus Biologicals (USA), antibody to vinculin were from Sigma (USA). The expression plasmid encoding H2O2 biosensor HyPer fused to the nuclear export sequence (HyPer-NES) was from Evrogen, Russia. DCF-DA was kindly donated by Dr. M.Yu. Menshikov (Cardiology Research Center, Moscow, Russia).
3
Apocynin Cytotoxicity Evaluation Protocol
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Apocynin (Acetovanillone, !98%) (Sigma Aldrich, MO), thiazolyl blue tetrazolium bromide (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide [MTT]) (Sigma Aldrich), 0.9% NaCl (Saline) (Adeka, Turkey), and dimethyl sulfoxide (DMSO) (Sigma Aldrich) were the main elements in this study. MTT was stored at 4°C, and apocynin, saline, and DMSO at room temperature. Apocynin dose, 20 mg/kg, was calculated based on the study of Keskin and Çakan. 23
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