2600 uv vis nir spectrophotometer

Morphology of folic-AIEgen was characterized on a FEI Tecnai F20 transmission electron microscopy (Hillsboro, OR, USA). Ultraviolet–visible–near-infrared (UV-vis-NIR) spectra of folic-AIEgen were recorded on a Shimadzu 2600 UV-vis-NIR spectrophotometer (Shimadzu, Kyoto, Japan). The hydrodynamic size was measured on a Malvern Zetasizer Nano-ZSE (Malvern, UK) at 25 °C.

A JEOL JEM-2100F transmission electron microscope was used to perform transmission electron microscopic investigations. A Rigaku X-ray diffractometer was used to perform X-ray powder diffraction investigations. A Shimadzu 2600 UV-vis-NIR spectrophotometer was used to record UV absorption spectra. An 808 nm LEO diode laser was used to perform photothermal investigations. A Malvern Zetasizer NanoZS was used to perform zeta potential investigations. A PerkinElmer Optima 3300DV was used to perform ICP-AES investigations. A BD FACSCalibur was used to perform flow cytometry.

Transmission electron microscopy (TEM) images were acquired on a JEOL JEM-2100F field emission electron microscope. Dynamic diameters and zeta potentials were assessed by a Malvern Zetasizer Nano ZS. UV-vis absorption spectra were detected by a Shimadzu 2600 UV-vis-NIR spectrophotometer. Fourier transform infrared (FTIR) spectra were determined by Bruker VERTEX 80V. Photoluminescence (PL) spectroscopy was obtained on a Shimadzu RF-6000 fluorescence spectrometer. Electron spin resonance (ESR) spectra were recorded on Brucker ELEXSYS spectrometer. OLYMPUS FV1000 was used to detect the confocal laser scanning microscopy (CLSM) images. Flow cytometry was performed by BD FACSCalibur Flow Cytometer. The fluorescence images of the mice were obtained on a two-dimensional InGaAs array (Princeton Instruments, NIRvana-640). UHPLC-MS/MS analyses were performed using a nanoElute UHPLC system (Bruker, Germany) coupled with a tims TOF pro2 mass spectrometer (Bruker, Germany) in Novogene Co., Ltd.