RNA was isolated using TriReagent (Sigma) as per the protocol of the manufacturer. It was subjected to DNaseI treatment followed by small RNA enrichment by the 4M LiCl method as given in Kansal et al. (2015 (link)). For cDNA synthesis, 2 μg of RNA was incubated with 100 pmoles of either oligodT primer (Eurofins Genomics) for total RNA or miR_oligodT primer (Eurofins Genomics) for small RNA using the SuperscriptII cDNA synthesis kit (Invitrogen). All the primers have been enlisted in Supplementary Table 1 .
Mir oligodt primer
Manufactured by Eurofins
The MiR_oligodT primer is a laboratory product used in molecular biology applications. It serves as a primer for the synthesis of complementary DNA (cDNA) from messenger RNA (mRNA) templates. The MiR_oligodT primer targets the poly(A) tail of mRNA molecules, enabling the initiation of reverse transcription and the generation of cDNA for downstream analyses.
Automatically generated - may contain errors
2 protocols using mir oligodt primer
1
RNA Isolation, DNase Treatment, and cDNA Synthesis
2
Small RNA Isolation and cDNA Synthesis
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RNA was isolated using TriReagent (Sigma) as per the manufacturer's protocol. It was subjected to DnaseI treatment followed by small RNA enrichment by 4 M LiCl method as given in (Kansal et al. 2015) (link). For cDNA synthesis, 2 μg of RNA was incubated with 100 pmoles of either oligodT primer (Eurofins Genomics) for total RNA or miR_oligodT primer (Eurofins Genomics) for small RNA using the SuperscriptII cDNA synthesis kit (Invitrogen). All the primers have been enlisted in Supplementary Table S1.
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