Sc 474

Tris-HCl was purchased from Sigma-Aldrich (St. Louis, MO, USA). Easy-Blue total RNA extraction kit was purchased from iNtRON biotechnology (Sungnam, South Korea). Anti-4-hydroxynonenal (4-HNE) (1:500; ab48506), glutathione peroxidase 4 (GPX4) (1:500; ab125066), caspase-1 (1:500; ab1872) and gasdermin D (GSDMD) (1:500; ab219800) were purchased from Abcam (Cambridge, UK). Anti-caspase-1 (p20) (1:500; AG-20B-0042-C100) was purchased Adipogen (San Diego, CA, USA). Anti-phosphorylated extracellular signal-regulated kinase (ERK) 1/2 (1:1000; cat. no. 9101L), c-Jun N-terminal kinase (JNK) (1:1000; cat. no. 9251L), and p38 (1:1000; cat. no. 9211L) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Total MAPK antibodies against ERK 1/2 (1:1000; sc-93), JNK (1:1000; sc-474) and p38 (1:1000; sc-535), inhibitory κ-Bα (Iκ-Bα; 1:1000; sc-371), β-actin antibody (1:1000; sc-1615), horseradish peroxidase (HRP)-conjugated secondary antibody, including chicken anti-rabbit IgG-HRP (1:5000; sc-516087), and loganin were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Loganin stock solutions were prepared with distilled water.