The content of monosaccharides in fucoidan was determined after acid hydrolysis. Five milligrams of fucoidan standard (Sigma-Aldrich. Co,. Spruce St. Louis, USA, Product of Australia) was dissolved in 1 ml HPLC grade water then treated with 1 ml of 4 M trifluoroacetic acid. The solution was heated for 2 hours at 100°C then filtered by 0.45 µm pore size filter (Merck KGaA, Darmstadt, Germany). The filtrate was neutralized with 0.1M NaOH and then evaporated with liquid nitrogen until the remaining volume of 0.5 ml. The content of monosaccharides of the fucoidan hydrolyzate was analyzed by High-Performance Liquid Chromatography (HPLC) (Shimadzu, SIL-10AD) using a refractive index detector (RID) equipped with a ligand-exchange column for Carbohydrate (Agilent Hi-Plex H, 7.7 × 100 mm) eluted with 0.005 M H 2 SO 4 at a flow rate of 0.7 ml/min. A sample of 20 µl was injected with separation time of 30 minutes. The standard monosaccharides used were fucose (Nacalai Tesque, Kyoto, Japan), glucose (Merck KGaA, Darmstadt, Germany), galactose (Merck KGaA, Darmstadt, Germany), rhamnose (Cica Reagen, Kanto Chemical, Tokyo, Japan), mannose (Merck KGaA, Darmstadt, Germany) and xylose (Merck KGaA, Darmstadt, Germany).
Fucose
Manufactured by Nacalai Tesque
Sourced in Japan
Fucose is a monosaccharide that serves as a core component in various glycans and glycoconjugates. It is a naturally occurring sugar found in many organisms and plays a vital role in diverse biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Mannose, by Merck Group (1 mentions)
Fucoidan standard, by Merck Group (1 mentions)
Hi plex h, by Agilent Technologies (1 mentions)
Sil 10ad, by Shimadzu (1 mentions)
Glucose, by Merck Group (1 mentions)
Galactose, by Merck Group (1 mentions)
Xylose, by Merck Group (1 mentions)
Glycerol, by Fujifilm (1 mentions)
Sucrose, by Fujifilm (1 mentions)
Glucose, by Nacalai Tesque (1 mentions)
Human hemoglobin, by Merck Group (1 mentions)
Glycine, by Nacalai Tesque (1 mentions)
Guanidine hydrochloride, by Nacalai Tesque (1 mentions)
Betaine, by Fujifilm (1 mentions)
Maltose, by Nacalai Tesque (1 mentions)
Galactose, by Nacalai Tesque (1 mentions)
L proline, by Peptide Institute (1 mentions)
D sorbitol, by Fujifilm (1 mentions)
Trehalose, by Tokyo Chemical Industry (1 mentions)
Levulose, by Fujifilm (1 mentions)
2 protocols using fucose
1
Monosaccharide Profiling of Fucoidan by HPLC
2
Characterization of Biomolecular Interactions
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NaH 2 PO 4 , Na 2 HPO 4 , levulose, xylose, sucrose, glycerol, betaine, D-sorbitol, and lactobionic acid were purchased from Wako Chemical (Japan). Human hemoglobin was purchased from Sigma-Aldrich. Guanidine hydrochloride, glucose, mannose, galactose, fucose, maltose, and glycine were purchased from Nacalai Tesque. Trehalose and glucosamine hydrochloride were purchased from Tokyo Chemical Industry. L-proline was purchased from Peptide Institute, Inc. (Japan). L-arginine hydrochloride was purchased from Kishida Chemical. UV-Vis spectra were recorded using a JASCO V-530 spectrometer with a bandwidth of 2.0 nm, data acquisition interval of 1 nm, and a range of 300-500 nm.
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