Fresh Li-Heparin-anticoagulated blood samples were kindly provided by the blood bank of the University of Tübingen. The study is approved by the ethics committee of the University of Tübingen (184/2003 V). The blood was centrifuged at 120 x g for 20 min at 21 °C and the platelets and leukocytes-containing supernatant was disposed. Erythrocytes were incubated for 48 hours at 37°C in vitro at a hematocrit of 0.4% in Ringer solution containing (in mM) 125 NaCl, 5 KCl, 1 MgSO 4 , 32 N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES; pH 7.4), 5 glucose, and 1 CaCl 2 . Where indicated, erythrocytes were exposed for 48 hours to Elvitegravir (MedChem Express, Princeton, USA). To test for an involvement of p38 kinase, erythrocytes were exposed for 48 hours to a combination of Elvitegravir and p38 kinase inhibitor SB203580 (Tocris bioscience, Bristol, UK). To test for an involvement of caspases, erythrocytes were exposed for 48 hours to a combination of Elvitegravir and pancaspase inhibitor zVAD (Tocris bioscience, Bristol, UK).
Bissinger R., Al Mamun Bhuyan A., Signoretto E, & Lang F. (2016). Stimulating Effect of Elvitegravir on Suicidal Erythrocyte Death. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 38(3).
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