Caproic

Lipase B from C. antarctica (CAL B) immobilized on acrylic resin, Novozym ® 435, was purchased from Novozymes (Bagsvaerd, Denmark). L-ascorbic acid 99.7 % pure was purchased from Zorka, Šabac, Serbia. Oleic acid (Ph. Eur., NF pure) was purchased from AppliChem, Darmstadt, Germany. Saturated fatty acidsacetic, caproic, capric, myristic, and stearic, were obtained from Sigma-Aldrich Chemie Gmbh, Steinheim, Germany, all 98 % pure. 2,2-Diphenyl-1-picrylhydrazyl radical (DPPH) and ascorbyl palmitate (≥ 99%) were purchased from Sigma-Aldrich Chemie Gmbh, as well. Following solvents were used as reaction media: t-butanol (99 %, Sigma-Aldrich Chemie Gmbh, Steinheim, Germany), acetone (99.5 %, Zorka Pharma, Šabac, Serbia), acetonitrile (p.a., Kemika, Zagreb, Croatia), n-hexane (95-98.5 %, Carlo Erba Reagents, Milano, Italy), and isooctane (99.5 % pure, Centrohem, Stara Pazova, Serbia). Hydranal ® -solvent and Hydranal ® -titrant were purchased from Sigma-Aldrich Chemie Gmbh, Steinheim, Germany. HPLC analyses were carried out with methanol and acetonitrile obtained from JT Baker, SAD, and phosphoric acid purchased from Sigma-Aldrich Chemie Gmbh, Steinheim, Germany, all of HPLC grade. Zeolite molecular sieves (diameter 2-3 mm and 0.4 nm pores) were used as water adsorbents.

Gelatin type A, wheat gluten, glycerol and sorbitol were obtained from Leiner Davis Gelatin, Rhodia, Synth and Getec, respectively (Brazilian suppliers). Caproic, caprylic, capric, lauric, myristic and palmitic fatty acids were obtained from Sigma-Aldrich (Brazil).