The chromaticity coordinates were measured with a UV-visible spectrophotometer Varian series Cary 50 Scan (Varian, Leini, TO, Italy), and data were processed analyzed using Cary Win UV Color Application V. 2.00 software. The transmittance of the whole visible spectrum (380-780 nm) was measured at a wavelength interval of 5 nm, using D65 illuminat and a 10° observation angle. Floral juices were used without any dilution, and transmittance was measured in a 5 mm quartz cuvette subtracting a blank (air). The pH measurements were performed with a CyberScan pH 510 Meter (Eutech Instruments, Landsmeer, The Netherlands) which was calibrated with standard buffer solutions of pH 7 and pH 4, prior to measuring the pH.
Cyberscan 510 ph meter
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The Cyberscan 510 pH meter is a compact and accurate pH measurement device designed for laboratory use. It features a large digital display, automatic temperature compensation, and the ability to store up to 100 data points. The Cyberscan 510 provides reliable pH measurement functionality for a variety of laboratory applications.
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7 protocols using cyberscan 510 ph meter
1
Chromaticity and pH Analysis of Floral Juices
2
pH Measurement Using InLab Easy Electrode
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The pH-value was determined by InLab Easy pH electrode (Mettler Toledo, Germany) with CyberScan pH 510 meter (Eutech Instruments, Thermo Scientific, Germany).
3
pH Measurement Using InLab Easy Electrode
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The pH-value was measured using an InLab Easy pH electrode (Mettler Toledo, Germany) with a CyberScan pH 510 meter (Eutech Instruments, Thermo Scientific, Germany).
4
Microbial Oxidation and Nitrogen Dynamics
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Carbon oxidation and ammonium stabilization were assessed in batch cultures by cultivating strain R31 in a basal medium (all units g/L): K2HPO4 14; KH2PO4 6; MgSO4·7H2O 0.2; trace mineral solution 2 mL; pH (at 25°C) = 7 ± 0.2. The trace mineral solution consisted of (all units g/L), EDTA 0.01; ZnSO4·7H2O 0.0001; CaCl2·2H2O 0.1; MnCl2·2H2O 0.008; FeCl3·6H2O 0.71; (NH4)6Mo7O24 0.00011; CuSO4·5H2O 0.0001; CoCl2·6H2O 0.2. The carbon (glucose) concentration was 1.251 g/L (carbon substrate 500 mg/L) while nitrogen (ammonium chloride) was 0.191 g/L (nitrogen substrate 50 mg/L) forming C/N ratio (w/w) of 10. The sterilized media were inoculated with 1% (v/v) of the isolate and incubated at 37°C with shaking (100 rpm) for 48 h. Aliquots were removed from each Erlenmeyer flask at four hour intervals and growth was recorded by measuring OD600. Next, the liquid was centrifuged at 10,000 rpm for 10 minutes and the supernatant was analyzed for COD (by closed reflux method using dichromate), ammonia, nitrite, and nitrate nitrogen by using an expandable ion electrode analyzer (Orion EA 940, Thermo Fisher Scientific, USA). Medium pH was measured using Cyberscan 510 pH meter (Eutech Instruments, Singapore). Hydroxylamine was measured spectrophotometrically according to Frear and Burrell [28 ]. Experiments were done thrice in triplicate sets.
5
Determination of Ionization Constants for Nitro Compounds
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Nitroacetamide (1 ) and N,N-dimethylnitroacetamide (3 ) have been obtained, respectively, by aminolysis from ethyl nitroacetate [32 (link),33 (link)] and methyl nitroacetate [34 (link)], following previously reported procedures.
Ionization constants of nitro compounds8 , 1 , and 3 were determined in water by potentiometric titration using a glass electrode (method of partial neutralization). The values of pH were determined with CyberScan510 pH meter produced by Eutech Instruments. Compound 8 was used as reference acid and its pK was determined to reproduce published results [12 (link)] using our procedure.
The values of p were calculated according to the formula:
where [HA] is the concentration of non-dissociated nitroacetamide and [A] is the concentration of its salt.
Ionization constants of nitro compounds
The values of p were calculated according to the formula:
where [HA] is the concentration of non-dissociated nitroacetamide and [A] is the concentration of its salt.
6
Electrochemical Characterization of Polymer Films
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All
electrochemical experiments,
namely, CV and DPV were carried out with Biologic science instrument
model: SP-150 s/n 0555 electrochemical workstation. The three-electrode
system contains a hollow PPy/AAS-modified GCE as the working electrode
and Ag/AgCl (saturated KCl) electrode and platinum wire were used
as the reference electrode and the counter electrode, respectively.
The pH measurements were carried out with Cyberscan 510 pH meter EUTECH
Instruments, Singapore. The surface morphology of polymer films was
studied by using Zeiss FESEM instrument, and SEM images were taken
from TESCAN Vega 3 instrument. EDX analysis with a Bruker QuantX EDS
detector was carried out. The wettability of the prepared film was
tested by using the contact angle DataPhysics Instrument.
electrochemical experiments,
namely, CV and DPV were carried out with Biologic science instrument
model: SP-150 s/n 0555 electrochemical workstation. The three-electrode
system contains a hollow PPy/AAS-modified GCE as the working electrode
and Ag/AgCl (saturated KCl) electrode and platinum wire were used
as the reference electrode and the counter electrode, respectively.
The pH measurements were carried out with Cyberscan 510 pH meter EUTECH
Instruments, Singapore. The surface morphology of polymer films was
studied by using Zeiss FESEM instrument, and SEM images were taken
from TESCAN Vega 3 instrument. EDX analysis with a Bruker QuantX EDS
detector was carried out. The wettability of the prepared film was
tested by using the contact angle DataPhysics Instrument.
7
Comprehensive Water Quality Analysis
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Physiochemical and microbiological analysis for all GW samples (n = 21) was conducted on the same day of collection. In situ measurement of electrical conductivity [17] (link) and pH was carried out using a CyberScan510 pH meter (Eutech Instruments, Thermo, Waltham, MA, USA). Total organic carbon (TOC) and total nitrogen (TN) were measured using a Multi N/C ® 2100S analyzer (Analytik Jena AG, Jena, Germany). Total suspended solids (TSS) and five-day biological oxygen demand (BOD 5 ) were determined according to standard analytical methods for the examination of water and wastewater [18, 19] (link).
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