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Synthetic lipid standards

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Synthetic lipid standards are pure, well-characterized lipid compounds used as reference materials in analytical and research applications. These standards provide a consistent and reliable source of lipids for the identification, quantification, and validation of lipid analysis techniques.

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Lab products found in correlation

Methanol, by Thermo Fisher Scientific (4 mentions) Ammonium formate, by Merck Group (2 mentions) Methanol, by Biosolve (2 mentions) 2 propanol, by Biosolve (2 mentions) Propan 2 ol, by Thermo Fisher Scientific (2 mentions) Chloroform, by Merck Group (2 mentions) Ammonium acetate, by Merck Group (2 mentions) Methyl tert butyl ether (mtbe), by Merck Group (2 mentions) Ammonium bicarbonate, by Merck Group (2 mentions) Norharmane, by Merck Group (1 mentions)

9 protocols using synthetic lipid standards

1

Synthetic Lipid Standards Acquisition

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Synthetic lipid standards were purchased from Avanti Polar Lipids and Larodan Fine Chemicals. CD3I was purchased from Cambridge Isotope Laboratories. All other chemicals and solvents were purchased from Sigma-Aldrich and Rathburn Chemicals.
Ejsing C.S., Bilgin M, & Fabregat A. (2015). Quantitative Profiling of Long-Chain Bases by Mass Tagging and Parallel Reaction Monitoring. PLoS ONE, 10(12), e0144817.
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2

Lipid Extraction and Quantification

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Methanol, 2-propanol and water were purchased from Biosolve BV (Valkenswaard, The Netherlands). Chloroform was from Rathburn Chemicals (Walkerburn, UK). Ammonium formate was from Sigma-Aldrich (Buchs, Switzerland). All solvents and chemicals were of the highest analytical grade. Synthetic lipid standards were purchased from Avanti Polar Lipids (Alabaster, AL, USA).
Sprenger R.R., Ostenfeld M.S., Bjørnshave A., Rasmussen J.T, & Ejsing C.S. (2023). Lipidomic Characterization of Whey Concentrates Rich in Milk Fat Globule Membranes and Extracellular Vesicles. Biomolecules, 14(1), 55.
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3

Synthetic Lipid Standards for Lipidomics

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Synthetic lipid standards were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). All used solvents were of at least HPLC grade. Stocks of internal standards were stored in glass ampoules at −20 °C until used for the preparation of internal standard mix in 10:3 MTBE/MeOH. 700 μl of internal standard mix contained the following: 1,778 pmol of cholesterol D7, 1,040 pmol of cholesterol ester 16:0 D5, 521 pmol of 50:0 TG D5, 145 pmol of 34:0 DG D5, 550 pmol of 25:0 PC, 435 pmol of LPC, 107 pmol of 25:0 PS, 295 pmol 25:0 PE, 85 pmol of 13:0 LPE, 192 pmol of 25:0 PI, 109 pmol of 25:0 PG, 73 pmol of 30:1 Cer, 123 pmol of 25:0 PA, 91 pmol of 13:0 LPA, 32 pmol of 13:0 LPI.
Gross A.S., Zimmermann A., Pendl T., Schroeder S., Schoenlechner H., Knittelfelder O., Lamplmayr L., Santiso A., Aufschnaiter A., Waltenstorfer D., Ortonobes Lara S., Stryeck S., Kast C., Ruckenstuhl C., Hofer S.J., Michelitsch B., Woelflingseder M., Müller R., Carmona-Gutierrez D., Madl T., Büttner S., Fröhlich K.U., Shevchenko A, & Eisenberg T. (2019). Acetyl-CoA carboxylase 1–dependent lipogenesis promotes autophagy downstream of AMPK. The Journal of Biological Chemistry, 294(32), 12020-12039.
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4

Lipid Standards for Metabolomic Analysis

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Synthetic lipid standards were purchased from Avanti Polar Lipids, Inc. (Alabaster, USA). All used solvents were of at least HPLC grade. Stocks of internal standards were stored in glass ampoules at −20 °C until used for the preparation of internal standard mix in MTBE/methanol (10/3 per vol). 700 µl internal standard mix contained: 1040 pmol cholesteryl ester 16:0 D7, 521 pmol 50:0 TG D5, 145 pmol 34:0 DG D5, 550 pmol 25:0 PC, 435 pmol LPC, 107 pmol 25:0 PS, 295 pmol 25:0 PE, 85 pmol 13:0 LPE, 192 pmol 25:0 PI, 109 pmol 25:0 PG, 73 pmol 30:1 Cer, 123 pmol 25:0 PA, 91 pmol 13:0 LPA, 32 pmol 13:0 LPI.
Rockenfeller P., Smolnig M., Diessl J., Bashir M., Schmiedhofer V., Knittelfelder O., Ring J., Franz J., Foessl I., Khan M.J., Rost R., Graier W.F., Kroemer G., Zimmermann A., Carmona-Gutierrez D., Eisenberg T., Büttner S., Sigrist S.J., Kühnlein R.P., Kohlwein S.D., Gourlay C.W, & Madeo F. (2017). Diacylglycerol triggers Rim101 pathway–dependent necrosis in yeast: a model for lipotoxicity. Cell Death and Differentiation, 25(4), 765-781.
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5

Analytical Grade Lipid Standards

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Water, propan-2-ol, and methanol were purchased from Fischer Scientific. Methyl tert-butyl ether, chloroform, ammonium bicarbonate, and ammonium acetate were purchased from Sigma–Aldrich. All chemicals were analytical grade. Synthetic lipid standards were purchased from Avanti Polar Lipids, Larodan Fine Chemicals, and Sigma–Aldrich.
Surma M.A., Herzog R., Vasilj A., Klose C., Christinat N., Morin-Rivron D., Simons K., Masoodi M, & Sampaio J.L. (2015). An automated shotgun lipidomics platform for high throughput, comprehensive, and quantitative analysis of blood plasma intact lipids. European Journal of Lipid Science and Technology, 117(10), 1540-1549.
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6

Analytical Standards for Lipid Analysis

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Chemicals and solvents of ACS (American Chemical Society) or LC–MS (Liquid Chromatography–Mass Spectrometry) grade were purchased from Sigma-Aldrich, and synthetic lipid standards were purchased from Avanti Polar Lipids.
Finkelstein S., Gospe SM I.I.I., Schuhmann K., Shevchenko A., Arshavsky V.Y, & Lobanova E.S. (2020). Phosphoinositide Profile of the Mouse Retina. Cells, 9(6), 1417.
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7

Lipid Standards Procurement and Preparation

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Water, propan-2-ol, and methanol were purchased from Fischer Scientific. Methyl tert-butyl ether, chloroform, ammonium bicarbonate and ammonium acetate were purchased from Sigma–Aldrich. All chemicals were analytical grade. Synthetic lipid standards were purchased from Avanti Polar Lipids, Larodan Fine Chemicals, and Sigma–Aldrich.
Grzybek M., Palladini A., Alexaki V.I., Surma M.A., Simons K., Chavakis T., Klose C, & Coskun Ü. (2019). Comprehensive and quantitative analysis of white and brown adipose tissue by shotgun lipidomics. Molecular Metabolism, 22, 12-20.
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8

Preparation of Synthetic Lipid Standards

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Synthetic lipid standards (see supplemental data, Common chemicals and lipid standards for complete list) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Individual standards were mixed and diluted with methyl-tert-butyl ether (MTBE)/methanol (MeOH) 10:3 (v/v) (see supplemental data, Common chemicals and lipid standards for details).
Vvedenskaya O., Rose T.D., Knittelfelder O., Palladini A., Wodke J.A., Schuhmann K., Ackerman J.M., Wang Y., Has C., Brosch M., Thangapandi V.R., Buch S., Züllig T., Hartler J., Köfeler H.C., Röcken C., Coskun Ü., Klipp E., von Schoenfels W., Gross J., Schafmayer C., Hampe J., Pauling J.K, & Shevchenko A. (2021). Nonalcoholic fatty liver disease stratification by liver lipidomics. Journal of Lipid Research, 62, 100104.
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9

Lipidomic Analysis of Synthetic Standards

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Methanol, 2‐propanol, and water were purchased from Biosolve BV (Valkenswaard, Netherlands). Chloroform was from Rathburn Chemicals (Walkerburn, UK). Ammonium formate and norharmane were from Sigma‐Aldrich (Buchs, Switzerland). All solvents and chemicals were HPLC grade. Synthetic lipid standards were purchased from Avanti Polar Lipids (Alabaster, USA).
Khalil S.M., Sprenger R.R., Hermansson M, & Ejsing C.S. (2022). DDA‐imaging with structural identification of lipid molecules on an Orbitrap Velos Pro mass spectrometer. Journal of Mass Spectrometry, 57(9), e4882.
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