Anti cd19 magnetic fluorobeads b

Anti-CD19 magnetic fluorobeads-B was purchased from One Lambda (Canoga Park, CA, USA). Akt inhibitor X and PP242 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Refolded human soluble BAFF (hsBAFF) was a recombinant form of the extracellular domain of the BAFF produced in Escherichia coli from this group [35 (link)]. RPMI 1640 medium and fetal bovine serum (FBS) were supplied by Gibco (Rockville, MD, USA). CellTiter 96®AQ_ueous One Solution Cell Proliferation Assay kit was from Promega (Madison, WI, USA). Enhanced chemiluminescence solution was from Sciben Biotech Company (Nanjing, China). 1,2-bis(oAminophenoxy) ethane-N,N,N’,N’-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA/AM) and 2-aminoethoxydiphenyl borane (2-APB) were purchased from Calbiochem (San Diego, CA, USA), whereas ethylene glycol tetra-acetic acid (EGTA) was purchased from Sigma (St. Louis, MO, USA). ML-SA1 was supplied by Abcam Technology (Cambridge, UK). Verapamil hydrochloride was from MedChemExpress (Monmouth Junction, NJ, USA). Rapamycin and KN93 were from ALEXIS (San Diego, CA, USA), while U0126 was from Sigma. Other chemicals were purchased from local commercial sources and were of analytical grade.

Anti-CD19 magnetic fluorobeads-B was purchased from One Lambda (Canoga Park, CA, USA). PP242 (mTORC1/2 kinase inhibitor) and Akt inhibitor X were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rapamycin (mTOR inhibitor) was from ALEXIS (San Diego, CA, USA). Refolded human soluble BAFF (hsBAFF) was a recombinant form of the extracellular domain of the BAFF produced in Escherichia coli from this group (Cao et al., 2005 (link)). RPMI 1640 was from Gibco (Rockville, MD, USA). Fetal bovine serum (FBS) was supplied by Hyclone (Logan, UT, USA). CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay kit was from Promega (Madison, WI, USA). Other chemicals were purchased from local commercial sources and were of analytical grade.

Anti-CD19 magnetic fluorobeads-B was purchased from One Lambda (Canoga Park, CA, USA). Metformin, U0126 and protease inhibitor cocktail were purchased from Sigma (St Louis, MO, USA), whereas Akt inhibitor X were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Rapamycin was from ALEXIS (San Diego, CA, USA). Refolded human soluble BAFF (hsBAFF) was a recombinant form of the extracellular domain of the BAFF produced in Escherichia coli from this group [34 (link)]. RPMI 1640 medium and fetal bovine serum (FBS) were from Gibco (Rockville, MD, USA). CellTiter 96®AQ_ueous One Solution Cell Proliferation Assay kit was from Promega (Madison, WI, USA). Enhanced chemiluminescence solution was supplied by Sciben Biotech Company (Nanjing, China). The following primary antibodies were used: phosphorylated Akt (p-Akt) (Ser473), p-S6K1 (Thr389), p-S6 (Ser235/236) (Cell Signaling Technology, Danvers, MA, USA), S6K1, S6, p-Erk1/2 (Thr202/Tyr204), Erk2 (Santa Cruz Biotechnology), p-PTEN (Thr366), PTEN (Epitomics, Burlingame, CA, USA), MKK1, mTOR (Sigma), Erk1/2, survivin, Akt, β-actin, FLAG, HA (Sciben Biotech Company); Goat anti-rabbit IgG-horseradish peroxidase (HRP), goat anti-mouse IgG-HRP, and rabbit anti-goat IgG-HRP (Sciben Biotech Company). Other chemicals used in this work were purchased from local commercial sources and were of analytical grade.