Polycarbonate porous membrane

LnCl₃·6H₂O (Ln = Y, Yb, Tm and Nd, 99%), Oleic acid (OA, 85%), 1-Octadecene (ODE, 90%), Cyclohexane (99%) and Poly (acrylic acid) (MW 2000) (PAA) were purchased from Aladdin, Inc (Shanghai, China). N-hydroxysuccinimide (NHS), N-(3dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) and avidin were obtained from Sangon Biotech Co., Ltd (Shanghai, China). Polycarbonate porous membrane and Avanti mini extruder were obtained from Avanti Polar Lipids. The RIPA lysis buffer, cell counting kit-8 (CCK-8), phenylmethanesulfonyl fluoride (PMSF) and the lipopolysaccharide (LPS, O55:B5) of E.coli were purchased from Beyotime Biotechnology (Beijing, China). PCB (Sigma-Aldrich, USA), T7 endonuclease 1 (T7E1), NLS-Cas9-EGFP and NLS-Cas9-NLS (Genscript Biotech, China) were commercially obtained. HPLC-purified oligonucleotides (Additional file 1: Table S1) were bought from Tsingke Biotechnology Co., Ltd. (Beijing, China). All chemicals were analytical grade. The medium (DMEM), fetal bovine serum (FBS) and antibiotics were purchased from Gibco (Rockville, MD). Aqueous solutions were prepared using Milli-Q water (18.2 MΩ, Millipore, USA).

PLGA, with a lactide-glycolide ratio of 50:50 and Mw of 7,000-17,000 (ester terminated), PEI (average Mw 25,000 Da), poly (vinyl alcohol) (PVA, 30-70 kDa) and Coumarin-6 were provided by Sigma Aldrich. DiR Iodide and DiO were obtained from Yeasen. PTX was purchased from J& K Chemical Technology. The Avanti mini extruder and polycarbonate porous membrane were obtained from Avanti Polar Lipids. Antibodies targeting Rb (4H1), p-AKT (D9E), AKT (40D4), E7 (F-7) and MDR1 (D-11) were bought from Cell Signaling Technology and Santa Cruz Biotechnology, respectively. AS1411 aptamer (Ap sequence: 5′‐SH‐C6‐TTGGTGGTGGTGGTTGTGGTGGTGGTGG‐3′, 28 bp) was synthetized by Sangon Bioengineering (Shanghai, China). siRNA sequences targeting E7 (sense 5′-GCGACUCAGAGGAAGAAAATT-3′, antisense 5′-UUUUCUUCCUCUGAGUCGCTT-3′) and Cy5-labeled siRNA-NC were synthetized by Shanghai GenePharma.
The human cervical cancer cell line HeLa was obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cells were cultured according to the guidelines recommended by the ATCC.
Female BALB/c nude mice were obtained from Shanghai Jiao Tong University School of Medicine and raised in the SPF animal facility. All the animal experimental procedures were conducted with the approval of the Animal Experimentation Ethics Committee of Jiao Tong University.