MeT-5A, HOMC-B1, DKO-MeT-5A, DKO-HOMC-B1, and Y-MESO-9 cells were cultured on glass coverslips and fixed using 4% paraformaldehyde solution for 20 min at room temperature. Cells were permeabilized with PBS containing 0.1% Triton X-100 and blocked using PBS containing 7% serum for 30 min. Then, cells were incubated with γ-catenin (desmoplakin; BD Transduction Laboratories, San Jose, CA, USA; 1:100 dilution) for 2 h at room temperature followed by fluorescence staining with anti-mouse IgG Alexa Fluor® 594 (Abcam, Cambridge, UK; 1:200 dilution) and Hoechst (Dojindo, Kumamoto, Japan; 1:200 dilution) for 1 h at room temperature. Images were acquired using BZ-II (Keyence) with a fluorescence microscope (BZ-X9000; Keyence).
Bz x9000
Manufactured by Keyence
Sourced in United States
The BZ-X9000 is a fluorescence microscope designed for high-resolution imaging. It features a compact and modular design, allowing for easy integration into various research and analysis workflows. The BZ-X9000 enables users to capture detailed images of samples while maintaining a concise and unbiased approach to its core function.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bz x9000
1
Immunofluorescence Staining of Cell Lines
2
Histomorphometric Analysis of Knee Joints
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The left knee joints were fixed in 10% phosphate-buffered saline formalin for two days, decalcified in 10% EDTA for three weeks, and embedded in paraffin. Sections were stained with Safranin-O -Fast green for measurement of articular cartilage thickness, subchondral bone plate thickness, subchondral trabecular bone number and diameter, and cartilage content using Bioquant Imaging software (Bioquant Imaging System, Nashville, VA USA) [51, 52] . Primary antibodies against NLRP3 (Abcam, Cambridge, MA, USA) were used with Alexa-Fluor 594 -conjugated secondary antibody for immunohistochemistry staining. The sections were counterstained with DAPI. Positively stained cells were counted using the cell count software in Keyence BZ-X9000 all-in-one imaging system (Itasca, IL, USA).
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