Muse cell count and viability kit

Manufactured by Merck Group

Sourced in United States

The Muse Cell Count and Viability kit is a compact, automated cell analysis system designed to provide accurate and reliable cell count and viability measurements. The kit utilizes advanced flow cytometry technology to analyze cell samples and generate key data points, including total cell count and percentage of viable cells.

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Lab products found in correlation

Muse cell analyzer, by Merck Group (2 mentions) Muse cell cycler, by Merck Group (2 mentions)

Close spelling variants of this product

Muse Count and Viability Kit Muse Count & Viability Kit Count and Viability Kit MuseTM

4 protocols using muse cell count and viability kit

Expansion of Tumor-Infiltrating Lymphocytes

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Total viable cells of 4–6×10⁶ of an FTD were plated in 2 wells of a 24-well plate (2–3×10⁶/well) with 2 mL of T-cell media per well, 4×10⁵ anti-CD3/anti-CD28 Dynabeads and 6000 IU/mL of IL-2. Splitting occurred as described in the panning protocol; however, half of the media was regularly required to be removed (slowly pipetting from the top of the well) and replaced with FM and IL-2 before splitting was indicated. 6000 IU/mL of IL-2 was maintained by bolus addition after each splitting or media replacement. Beads were removed at the completion TIL expansion (day 14).
All cell counts in this study were performed using a Muse Cell Analyzer (MilliporeSigma) and the Muse Cell Count and Viability kit.

Braun M.W., Abdelhakim H., Li M., Hyter S., Pessetto Z., Koestler D.C., Pathak H.B., Dunavin N, & Godwin A.K. (2020). Adherent cell depletion promotes the expansion of renal cell carcinoma infiltrating T cells with optimal characteristics for adoptive transfer. Journal for Immunotherapy of Cancer, 8(2), e000706.

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Cell Viability Assay with Pneumolysin

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Cell viability was assessed using the Muse Cell Analyzer (Millipore Sigma). HLMVECs were seeded at a density of 6 × 10⁵ in 6-well plates with complete EBM-2 MV culture medium with or without 30 µM GGA or DMSO for 6 h. PLY was added to dishes 4 h before the viability test. The percentage of live HLMVECs was determined using the Muse cell count and viability kit (Millipore Sigma). In brief, 50 µl of suspended HLMVECs was mixed with 450 µl of count and viability reagent, gently mixed, and injected into the Muse Cell Analyzer. Statistical analysis was performed based on three independent experiments. p Value was compared to the cell treated with GGA plus PLY group.

Li X., Yu Y., Gorshkov B., Haigh S., Bordan Z., Weintraub D., Rudic R.D., Chakraborty T., Barman S.A., Verin A.D., Su Y., Lucas R., Stepp D.W., Chen F, & Fulton D.J. (2018). Hsp70 Suppresses Mitochondrial Reactive Oxygen Species and Preserves Pulmonary Microvascular Barrier Integrity Following Exposure to Bacterial Toxins. Frontiers in Immunology, 9, 1309.

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Evaluating MC3T3-E1 Cell Viability

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Cell viability and total cell numbers were measured by flow cytometric analysis. Briefly, MC3T3-E1 cells were seeded at 3 × 10³ cells/mL in six well plates for overnight and treated with the different concentrations (0–200 μg/mL) of FO for 1, 3, 5, and 7 days. DEX (100 nM) was used a positive control for MC3T3-E1 cell viability. After harvesting, the cells were washed with ice-cold phosphate-buffered saline (PBS) and incubated with Muse^® cell count and viability kit (EMD Millipore, Billerica, MA, USA) for 5 min. Cell viability and total cell number were measured by Muse^® cell cycler (EMD Millipore).

Molagoda I.M., Karunarathne W.A., Choi Y.H., Park E.K., Jeon Y.J., Lee B.J., Kang C.H, & Kim G.Y. (2019). Fermented Oyster Extract Promotes Osteoblast Differentiation by Activating the Wnt/β-Catenin Signaling Pathway, Leading to Bone Formation. Biomolecules, 9(11), 711.

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Flow Cytometry-based Cell Viability Assay

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To estimate viability, total viable cell count and dead cell percentage, flow cytometry analysis was carried out based on differential staining of viable and non-viable cells due to their different permeability to DNA binding dyes. B16F10 cells were plated at a density of 1 × 10⁴ cell/mL for 18 h and then treated with the indicated concentrations (0–800 μg/mL) of PS and PTS for 72 h. In brief, the cells were harvested and washed with ice-cold phosphate-buffered saline (PBS). Next, the cells were incubated with Muse® cell count and viability kit (EMD Millipore, Billerica, MA, USA) for 5 min and cell viability, total cell count and dead cell population were analyzed by using a Muse® cellcycler (EMD Millipore) according to the manufacturer’s instructions.

Karunarathne W.A., Molagoda I.M., Park S.R., Kim J.W., Lee O.K., Kwon H.Y., Oren M., Choi Y.H., Ryu H.W., Oh S.R., Jo W.S., Lee K.T, & Kim G.Y. (2019). Anthocyanins from Hibiscus syriacus L. Inhibit Melanogenesis by Activating the ERK Signaling Pathway. Biomolecules, 9(11), 645.

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