1 2 dioleoyl sn glycero 3 phosphoglycerol

Manufactured by Avanti Polar Lipids

Sourced in United States

1,2-dioleoyl-sn-glycero-3-phosphoglycerol is a phospholipid compound that can be used in various laboratory applications. It is a key component in the synthesis and study of biological membranes. The compound has a glycerol backbone with two oleic acid chains and a phosphoglycerol head group.

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Lab products found in correlation

1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (10 mentions) 1 2 dioleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (2 mentions) Sm2 bio beads, by Bio-Rad (2 mentions) Mtset, by Biotium (2 mentions) Milli q, by Merck Group (2 mentions) Nahco3, by Thermo Fisher Scientific (2 mentions) Squalene, by Thermo Fisher Scientific (2 mentions) 1 2 dipalmitoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (2 mentions) Cy5 dope, by Avanti Polar Lipids (1 mentions) 1 2 distearoyl sn glycero 3 phosphoethanolamine n biotinyl polyethylene glycol 2000 dspe peg biotin, by Avanti Polar Lipids (1 mentions) 1 3 bis 1 2 dioleoyl sn glycero 3 phospho glycerol, by Avanti Polar Lipids (1 mentions) 1 2 dioleoyl sn glycero 3 phosphocholine dopc, by Avanti Polar Lipids (1 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions) Cacl2, by Merck Group (1 mentions) N hexadecane, by Thermo Fisher Scientific (1 mentions) α hemolysin, by Merck Group (1 mentions) 1 2 diphytanoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Octadecyltrichlorosilane, by Thermo Fisher Scientific (1 mentions) Alamethicin, by Merck Group (1 mentions)

12 protocols using 1 2 dioleoyl sn glycero 3 phosphoglycerol

Lipid Composition for Liposome Formulations

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1,2-Dioleoyl-sn-glycero-3-phosphocholine
(DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
(DOPE), 1,2-dioleoyl-sn-glycero-3phosphoglycerol
(DOPG), 1′,3′-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol
(18:1 cardiolipin), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000 (DSPE-PEG-biotin),
1,2-dioleoyl-sn-glycero-3-(cytidine diphosphate)
(CDP-DAG), and DOPE-Cy5 were from Avanti Polar Lipids.

Godino E., Restrepo Sierra A.M, & Danelon C. (2023). Imaging Flow Cytometry for High-Throughput Phenotyping of Synthetic Cells. ACS Synthetic Biology, 12(7), 2015-2028.

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DOPC and DOPG Lipid Acquisition

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Phospholipids 1,2-dioleoyl-sn-glycero-3-phosphocholine (C18:1, DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphoglycerol (C18:1, DOPG) were purchased from Avanti Polar Lipids, Inc.

Shukla R., Medeiros-Silva J., Parmar A., Vermeulen B.J., Das S., Paioni A.L., Jekhmane S., Lorent J., Bonvin A.M., Baldus M., Lelli M., Veldhuizen E.J., Breukink E., Singh I, & Weingarth M. (2020). Mode of action of teixobactins in cellular membranes. Nature Communications, 11, 2848.

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Lipid Preparation for Biophysical Studies

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1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3phosphoglycerol (DOPG) and 1′,3′-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol (18:1 CL) were purchased from Avanti Polar Lipids as chloroform solutions.

Godino E., Doerr A, & Danelon C. (2022). Min waves without MinC can pattern FtsA-anchored FtsZ filaments on model membranes. Communications Biology, 5, 675.

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Reconstitution of Lipid Membranes

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1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphoglycerol (DOPG) were obtained from Avanti Polar Lipids Inc. (USA). SM2 Bio-Beads (mesh size 20-50) were purchased from BioRad. MTSET was purchased from Biotium. All other compounds were purchased from Sigma-Aldrich (UK).

Haylock S., Friddin M.S., Hindley J.W., Rodriguez E., Charalambous K., Booth P.J., Barter L.M, & Ces O. (2020). Membrane protein mediated bilayer communication in networks of droplet interface bilayers. Communications Chemistry, 3, 77.

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Liposome Preparation using DOPC and DOPG

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1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphoglycerol (DOPG) were obtained from Avanti Polar Lipids Inc. (USA). SM2 Bio-Beads (mesh size 20–50) were purchased from Bio-Rad. MTSET was purchased from Biotium. All other compounds were purchased from Sigma-Aldrich (UK).

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Lipid Bilayer Formation Reagents

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The phospholipids 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoglycerol (DOPG), 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC), and 1,2dilauroyl-sn-glycero-3-phosphocholine (DLPC) were obtained from Avanti Polar Lipids. Squalene, n-hexadecane, and octadecyltrichlorosilane (OTS) were purchased from Acros Organics. NaCl (99.99%, metals basis) and NaHCO 3 were obtained from Alfa Aesar. CaCl 2 , α-hemolysin, and alamethicin was purchased from Sigma-Aldrich. Ultra-pure water (Milli-Q, Merck-Millipore, resistivity < 18.2 MΩ•cm) was used to make all buffers.

Beltramo P.J., Scheidegger L, & Vermant J. (2018). Toward Realistic Large-Area Cell Membrane Mimics: Excluding Oil, Controlling Composition, and Including Ion Channels. Langmuir : the ACS journal of surfaces and colloids, 34(20).

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Lipid Composition and Characterization

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Ray S., Reyes S., Xiao C, & Sun J. (2019). Effects of membrane lipid composition on Mycobacterium tuberculosis EsxA membrane insertion: a dual play of fluidity and charge. Tuberculosis (Edinburgh, Scotland), 118, 101854.

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Liposome Characterization with Fluorescent Probes

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Span 60, Span 80, Span 85, Tween 60, Tween 80, calcein, ETH-157, L-ornithine monohydrochloride and L-arginine were obtained from Sigma-Aldrich. 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phosphoglycerol (DOPG), and cholesterol were purchased from Avanti Polar Lipids. Laurdan and 8-hydroxy-1,3,6-pyrenetrisulfonate (pyranine) were obtained from Thermo Fisher Scientific. Melittin and alamethicin were from Serva Electrophoresis and Santa Cruz Biotechnology, respectively, and obtained via Bio-Connect Life Sciences. Radiolabeled L-[¹⁴C(U)]-arginine was ordered from Moravek, Inc.

Bartelds R., Nematollahi M.H., Pols T., Stuart M.C., Pardakhty A., Asadikaram G, & Poolman B. (2018). Niosomes, an alternative for liposomal delivery. PLoS ONE, 13(4), e0194179.

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Probing Lipid Membrane Interactions with Fluorescent Peptides

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5(6)-carboxyfluorescein (CF) and CL-2B Sepharose were from Sigma (St. Louis, MO). 6-lauroyl-2-dimethylamino naphthalene (Laurdan) was from Molecular Probes (Invitrogen, Eugene, OR). 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (POPG), 1’,3’-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol (Cardiolipin, TOCL), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2–1,3-benzoxadiazol-4-yl) (DPPE-NBD), and 1-lauroyl-2-(1-pyrenebutanoyl)-sn-glycero-3-phosphocholine (Pyr-PC) were from Avanti Polar Lipids (Alabaster, AL). Peptides (horine: WWWLRRRW and verine-L: RRRWWWWL) were chemically synthesized and purified > 95% (Genemed Synthesis, TX). Some calculated properties of these peptides are given in Table S1. All reagents were used as received.

Bozelli JC J.r., Yune J., Dang X., Narayana J.L., Wang G, & Epand R.M. (2020). Membrane activity of two short Trp-rich amphipathic peptides. Biochimica et biophysica acta. Biomembranes, 1862(7), 183280.

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Antibiotic Susceptibility and Cytotoxicity Assays

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The A. baumannii strain (CECT 452; ATCC 19606) and Pseudomonas aeruginosa strains (CECT 4122; ATCC 15692) are from the Spanish Type Culture Collection (CECT). The Escherichia coli BL21(DE3) strain and the pET11c plasmid are from Novagen. MRC-5 and HepG2 cells are from the American Type Cell Culture Collection (ATCCC). Mueller–Hinton broth, LPS and RNase A (Type XII) are from Sigma-Aldrich. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT), Isopropyl β-D-1-thiogalactopyranoside (IPTG) and colistin are from Apollo Scientific. 1-aminonaphthalene-3,6,8-trisulfonate (ANTS), α,α′-dipyridinium p-xylene dibromide (DPX) and the fluorescent probe BODIPY TR cadaverine are from Molecular Probes. Toludine blue is from Merck. RNase 3/1 gene was purchased from NZYTech. 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphoglycerol (DOPG) were from Avanti Polar Lipids. E. coli lipid extract was obtained as described (Folch et al., 1957 (link)). Human RNase 1 gene was a gift from Dr. Maria Vilanova, Universitat de Girona, Spain, and human RNase 3 sequence was taken from a previously synthesized gene (Boix et al., 1999 (link)).

Prats-Ejarque G., Li J., Ait-Ichou F., Lorente H, & Boix E. (2019). Testing a Human Antimicrobial RNase Chimera Against Bacterial Resistance. Frontiers in Microbiology, 10, 1357.

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