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Chl 1

Manufactured by American Type Culture Collection
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The CHL-1 is a laboratory equipment designed for cell culture applications. It is a compact and efficient incubator that maintains optimal temperature, humidity, and atmospheric conditions for cultivating cells. The CHL-1 provides a controlled environment to support the growth and maintenance of various cell lines.

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Lab products found in correlation

Sk mel 2, by American Type Culture Collection (5 mentions) Sk mel 28, by American Type Culture Collection (5 mentions) A2058, by American Type Culture Collection (5 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Sk mel 5, by American Type Culture Collection (4 mentions) Hema lp, by Thermo Fisher Scientific (3 mentions) Uacc903, by American Type Culture Collection (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Hek293t, by American Type Culture Collection (2 mentions) Mycoalert mycoplasma detection kit, by Lonza (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Mda mb 231, by American Type Culture Collection (2 mentions) Medium 254, by Thermo Fisher Scientific (2 mentions) Dmem medium, by Thermo Fisher Scientific (2 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Rpmi 1640 media, by Thermo Fisher Scientific (2 mentions) Chl 1, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)

26 protocols using chl 1

1

Culturing Melanoma Cell Lines

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The A375, MEL39, and CHL1 melanoma cell lines were purchased from the ATCC. A375 and CHL1 were cultured in Dulbecco’s Modified Eagles Medium (DMEM) supplemented with 10% fetal bovine serum (FBS). MEL39 was cultured in RPMI supplemented with 10% FBS. All cell lines were cultured and maintained in 5% CO2 at 37°C.
DiVincenzo M.J., Barricklow Z., Schwarz E., Moufawad M., Howard J.H., Yu L., Chung C., Gru A.A, & Carson WE I.I.I. (2021). Loss of miR-1469 expression mediates melanoma cell migration and invasion. PLoS ONE, 16(9), e0256629.
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2

Cell Culture Protocol for Cancer Research

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All cell lines and primary cells were cultured at 37 °C, 5% CO2. HEK-293T, SK-MEL-5 and CHL-1 were cultured on DMEM 10% FBS, 1% Pen-Strep; HT-1376, Caco-2, SK-MEL-2 and LS174T were cultured in EMEM 20% FBS, 1% Pen-Strep; BxPC-3, MM415, MM485, AsPC-1, DLD-1 KRASWT/−, and DLD-1 KRASG13D/− were cultured in RMPI-1460 (ATCC Modification) 10% FBS, 1% Pen-Strep; T24 and Capan-2 were cultured in McCoy’s 5A (Modified) 10% FBS, 1% Pen-Strep. CHL-1, HT-1376, Caco-2, Capan-2, SK-MEL-2, SK-MEL-5, LS174T, AsPC1 and T24 lines were purchased authenticated from ATCC. DLD-1 KRASWT/−, and DLD-1 KRASG13D/− were purchased from Horizon Discovery, Ltd. MM415 and MM485 were authenticated by polymorphic short tandem repeat (STR) loci profiling by Promega. HEK-293T, CHL-1, SK-MEL-5, BxPC-3, AsPC-1, LS174T, HT-1376 and T24 are derived from female individuals. Caco-2, DLD-1, MM415, MM485, Capan-2 and SK-MEL-2 are derived from male individuals.
Primary human melanocytes were isolated from fresh biopsy samples approved by the Stanford University IRB and were propagated up to eight passages in Media 254 with HMGS supplement (Thermo Fisher Scientific Scientific) and 1% Pen-Strep. All primary human melanocytes were male. All cell lines and primary cells were confirmed to be mycoplasma free bimonthly using the MycoAlert mycoplasma detection kit (Lonza).
Kovalski J.R., Bhaduri A., Zehnder A.M., Neela P.H., Che Y., Wozniak G.G, & Khavari P.A. (2019). The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2. Molecular cell, 73(4), 830-844.e12.
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3

Culturing BRAFV600E Melanoma Cell Lines

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SK-MEL-28 (BRAFV600E positive) and CHL-1 human cell lines were purchased from ATCC (Alexandria, MN, USA) and cultured in high-glucose DMEM. B16F10-nex cells were generously provided by Dr. Luiz Rodolpho R. G. Travassos (Federal University of São Paulo, São Paulo, Brazil). All cells were cultured after 20–30 passages at 37 °C in a sterile humidified atmosphere with 5% CO2 and 95% air, supplemented with 10% fetal bovine serum (Sigma-Aldrich, Darmstadt, Germany), 100 mg/mL streptomycin, and 100 U/mL penicillin (Gibco-Thermo Fisher Scientific Inc., Waltham, MA, USA). The medium was refreshed every three days, and cells were used for experiments or cryopreservation before reaching 90% confluence. All cells previously tested negative for mycoplasma contamination (VenorTMGeM Mycoplasma Detection Kit, PCR-based, MP0025, Sigma-Aldrich) (Figure S6).
Bonturi C.R., Salu B.R., Bonazza C.N., Sinigaglia R.D., Rodrigues T., Alvarez-Flores M.P., Chudzinski-Tavassi A.M, & Oliva M.L. (2022). Proliferation and Invasion of Melanoma Are Suppressed by a Plant Protease Inhibitor, Leading to Downregulation of Survival/Death-Related Proteins. Molecules, 27(9), 2956.
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4

Melanoma Cell Culture and Maintenance

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WM35, WM115, WM793, and WM3670 were kindly provided by Dr. Meenhard Herlyn (Wistar Institute, Philadelphia, PA). Mel624, CHL-1, SK-mel30, and B16F10 cells were purchased from ATCC or provided by the Comprehensive Cancer Center Core Facilities at the University of Chicago. Melanoma cells were maintained in DMEM (Dulbecco’s modified Eagle’s medium) medium (Invitrogen) supplemented with fetal bovine serum (FBS, 10% HyClone,), penicillin (100 U/ml), and streptomycin (100 μg/ml, Invitrogen, Carlsbad, CA). NHEM cells (Normal Human Epidermal Melanocytes) were purchased form Lonza and cultured in 95% air and 5% CO2 at 37 °C in NHEM defined medium obtained from Lonza according to the manufacturer’s instructions.
Yang S., Wei J., Cui Y.H., Park G., Shah P., Deng Y., Aplin A.E., Lu Z., Hwang S., He C, & He Y.Y. (2019). m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade. Nature Communications, 10, 2782.
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5

Cell Culture Practices for Disease Research

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CHL-1, HeLa, MDA-MB 231, HACAT and HMCB cells were procured from ATCC. The first 4 were cultured in DMEM with 10% FBS, whereas HMCB cells were cultured in EMEM. All cells were grown with 10% FBS and cultured at 37 °C with 5% CO2. Cells were routinely monitored for mycoplasma contamination.
Kumar V., Sabaté-Cadenas X., Soni I., Stern E., Vias C., Ginsberg D., Romá-Mateo C., Pulido R., Dodel M., Mardakheh F.K., Shkumatava A, & Shaulian E. (2024). The lincRNA JUNI regulates the stress-dependent induction of c-Jun, cellular migration and survival through the modulation of the DUSP14-JNK axis. Oncogene, 43(21), 1608-1619.
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6

Culturing Human Melanoma and Melanocyte Cells

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Human melanoma cell lines (A2058, SK-MEL-28, CHL-1, and A375) and the human epidermal melanocyte cell line HEMa-LP were all from ATCC (ATCC, USA). Human melanoma cell lines were cultured in DMEM medium (Gibco, USA) containing 10% fetal bovine serum (FBS) (Invitrogen, USA). HEMa-LP cells were maintained in 254 medium (Cascade Biologics, USA). All cell lines were maintained in a 37 °C cell incubator containing 5% CO2. Cell transfection was performed with Lipofectamine 3000 (Invitrogen).
Xie Y, & Chen G. (2022). LncRNA SNHG12 promotes the malignant progression of melanoma by targeting miR-199b. Annals of Translational Medicine, 10(4), 226.
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7

Comprehensive Protocols for Cancer Cell Lines

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All cells were incubated at 37°C in humidified conditions with 5% CO2. Human cancer cell lines (CHL-1, HMCB, A2058, and SK-MEL-5 from ATCC, Manassas, VA; WM3311, and WM3482 from Rockland Immunochemicals Inc., Limerick, PA; parental MCF10A, isogenic BRAFV600E knockin MCF10A, parental RKO, and isogenic BRAF V600E mutant alleles knockout RKO, and engineered RKO with flag-tag knockin from Horizon Discovery (Saint Louis, MO) were cultured in cell culture medias suggested by manufacturer. HEK293T cells (ATCC, Manassas, VA) were maintained in regular Dulbecco’s Modified Eagle’s Medium (DMEM, Corning, 10013CV), supplemented with 10% fetal bovine serum (FBS, Sigma, F0926) and 1x penicillin/streptomycin solution (Corning, 3001CI), or in phenol red free DMEM (HyClone, SH30284.01) throughout the screening. For drug treatment for functional studies, cells were treated with either vehicle or chemicals at indicated concentration.
Mo X., Niu Q., Ivanov A.A., Tsang Y.H., Tang C., Shu C., Li Q., Qian K., Wahafu A., Doyle S.P., Cicka D., Yang X., Fan D., Reyna M.A., Cooper L.A., Moreno C.S., Zhou W., Owonikoko T., Lonial S., Khuri F.R., Du Y., Ramalingam S.S., Mills G.B, & Fu H. (2022). Systematic discovery of mutation-directed neo-protein-protein interactions in cancer. Cell, 185(11), 1974-1985.e12.
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8

Culturing Murine and Human Melanoma Cells

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B16-F0 (ATCC CRL-6322) murine melanoma cells and CHL-1 (ATCC) human melanoma cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Sigma) supplemented with antibiotics (P/S, Sigma), 10% foetal bovine serum (Gibco), and 2 mmol/L glutamine (Sigma). Cells were grown in tissue culture flasks at 37 °C in a 5% CO2 humidified environment. B16 KO and CHL-1 KO cells were generated as described in our previous work (D’Amore et al., 2020) [5 ].
Hanbashi A., Alotaibi M., Sobeai H.M., Binobaid L., Alhazzani K., Jin X., Kamli F., Alhoshani A, & Parrington J. (2023). Loss of two-pore channel 2 function in melanoma-derived tumours reduces tumour growth in vivo but greatly increases tumour-related toxicity in the organism. Cancer Cell International, 23, 325.
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9

Cytotoxicity Assay for Melanoma Cells

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A375 (CRL-1619) and CHL-1 (CRL-9446) were purchased from ATCC on 11/5/2014 and 11/18/2014 respectively. A375SM was provided by Prof. Isiah Fidler (MD Anderson, Texas) on 10/30/2014. All cell lines except B16-F10, H460, and HCT 116 were cultured in DMEM supplemented with 10% FBS (Gemini). B16-F10, H460, and HCT 116 were cultured in RPMI with 10% FBS. Vemurafenib, trametinib and Annexin V-FITC (10040-02) were purchased from LC Laboratories, MedChemExpress, and SouthernBiotech respectively. The following antibodies were purchased from Cell Signalling Technology: anti-PARP-1 (9542), anti-caspase-3 (9662), anti-β-actin (4967), anti-phospho-ERK1/2 (Thr202/Tyr204) (4370), anti-ERK1/2 (4695) and anti-rabbit IgG HRP linked (7074). Anti-cleaved-PARP-1 (ab32561) antibody was purchased from Epitomics. PAC-1 and PAC-1a were synthesized as previously reported.(34 (link))
Peh J., Fan T.M., Wycislo K.L., Roth H.S, & Hergenrother P.J. (2016). The combination of vemurafenib and procaspase-3 activation is synergistic in mutant BRAF melanomas. Molecular cancer therapeutics, 15(8), 1859-1869.
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10

Cell Line Culture Protocols

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A549 (Cat# CCL-185), MDA-MB-231 (Cat# HTB-26), SW620 (Cat# CCL-227), MEWO (Cat# HTB-65), SK-MEL-28 (Cat# HTB-72), CHL-1 (Cat# CRL-9446) and WI-38 (Cat# CCL-75) cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA) and were cultured in complete Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS (Atlanta Biologicals, Flowery Branch, GA, USA) and 1% penicillin-streptomycin solution (Thermo Fisher Scientific, Waltham, MA, USA). MyLa1929 cells were kindly provided by Dr. David Weinstock in the Dana-Farber Cancer Institute and cultured in RPMI 1640 media (Life Technologies, Carlsbad, CA, USA) supplemented with 10% FBS (Atlanta Biologicals, Flowery Branch, GA, USA) and 1% penicillin-streptomycin solution (Thermo Fisher Scientific, Waltham, MA, USA).
Zhang X., He Y., Zhang P., Budamagunta V., Lv D., Thummuri D., Yang Y., Pei J., Yuan Y., Zhou D, & Zheng G. (2020). Discovery of IAP-Recruiting BCL-XL PROTACs as Potent Degraders across Multiple Cancer Cell Lines. European journal of medicinal chemistry, 199, 112397.
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