Thrombelastograph hemostasis analyzer system

Five weeks after thrombus operation, femoral venous blood samples of pigs in control, cirrhosis, and cirrhosis-PVT groups were collected for hematologic parameter testing of hepatic and coagulation functions following the standard procedures. Biochemical analyses and coagulation screening tests were measured with the spectrophotometric analysis and the turbidimetric method, respectively. Thrombelastography (TEG) was performed using Thrombelastograph Hemostasis Analyzer System (Haemonetics Corporation, USA). Thromboelastographic parameters consisted of clot time (R time, the time from the start of sample tracing till the first TEG trace amplitude reaching 2 mm), clot formation time (K time, the time from the measurement of R to the formation of fixed clot firmness), rate of clot formation (α angle, the speed of fibrin buildup and crosslinking), maximum amplitude (MA, the maximum amplitude on the TEG tracing), and coagulation index (CI, derived from R, K, α angle, and MA). Herein, R denotes the rate of initial fibrin formation, K represents the clot kinetics, α angle is the fibrinogen level, MA reflects the strength of the clot, and CI describes the overall coagulation. The serum levels of interleukin 6 (IL-6), IL-8, and tumor necrosis factor alpha (TNF-α) were detected with a chemiluminescent analyzer (IMMULITE 1000 Immunoassay System, Siemens Healthineers, Germany).

Thromboelastography (TEG) (TEG 5000, Thrombelastograph Hemostasis Analyzer System, Haemonetics Corporation, Braintree, MA, USA) was performed to assess coagulation profile. Clot formation was triggered using Kaolin in vitro. The variables of TEG include rate of clot formation (α angle), maximum amplitude (MA), clot time (R), clot formation time (K), and coagulation index (CI).