U87 glioma cells

Manufactured by American Type Culture Collection

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The U87 glioma cells are a well-characterized human brain tumor cell line derived from a malignant glioblastoma. These cells are commonly used in research to study the biology and behavior of glioblastoma, a type of brain cancer.

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U87 cells (37 citations) U87 human glioma cells (4 citations) H3 wild-type adult glioma cells (U87) (4 citations)

5 protocols using u87 glioma cells

Glioma Cells and Glioblastoma Stem Cell Culture

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Human U87 glioma cells were obtained from American Type Culture Collection (ATCC, Manassas, VA) and grown in complete Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10 % fetal calf serum at 37 °C and 5 % CO₂. Human GSCs MGG4, MGG8, MGG18, BT74 were isolated as previously described [28 (link),30 (link)], and maintained as spheres in serum-free medium containing 20 ng/mL recombinant human EGF (R&D systems) and 20 ng/mL recombinant human FGF2 (Peprotech). GSCs were passaged by dissociating neurospheres using the Neuro-Cult Chemical Dissociation Kit (StemCell Technologies). Mouse 005 GSCs were provided by Dr. I. Verma (Salk Institute for Biological Studies, La Jolla, CA) [33 (link), 34 (link)]. Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza. Human brain microvascular endothelial cells (HBMECs) were obtained from Dr. Ken Arai (MGH). Axitinib (Pfizer Inc) was provided by Pfizer and dissolved in DMSO as a 25 mM stock solution for in vitro studies. The final concentrations added to cells had less than 0.5 % DMSO, which was nontoxic to cells.

Lu L., Saha D., Martuza R.L., Rabkin S.D, & Wakimoto H. (2014). Single agent efficacy of the VEGFR kinase inhibitor axitinib in preclinical models of glioblastoma. Journal of neuro-oncology, 121(1), 91-100.

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Glioma Cell Line Cultivation

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Human glioma cell lines (LN229 and U251) were kindly supplied by Dr. Frank Furnari. The U87 glioma cells were purchased from American Type Culture Collection. Glioma cells were propagated in DMEM (Cellgro) supplemented with 10% fetal bovine serum. All cell lines were grown in 100 U/ml penicillin and 0.1 mg/ml streptomycin in a humidified incubator at 37°C, 5% CO 2 atmosphere.

Golden E.B., Cho H.Y., Hofman F.M., Louie S.G., Schönthal A.H, & Chen T.C. (2015). Quinoline-based antimalarial drugs: a novel class of autophagy inhibitors. Neurosurgical focus, 38(3).

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Culturing U87 Glioma and HEK 293T Cells

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U87 glioma cells and HEK 293T cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Invitrogen Life Technologies, Carlsbad, CA, USA). SYBR Green PCR master mix and the TaqMan microRNA reverse transcription kit were purchased from Applied Biosystems (Foster City, CA, USA). YC-1 was obtained from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in dimethyl sulfoxide.

NIE Q.M., LIN Y.Y., YANG X., SHEN L., GUO L.M., QUE S.L., LI X.X., GE J.W., WANG G.S., XIONG W.H., GUO P, & QIU Y.M. (2015). IDH1R132H decreases the proliferation of U87 glioma cells through upregulation of microRNA-128a. Molecular Medicine Reports, 12(5), 6695-6701.

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Culturing Lung and Glioma Cells

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H460 non-small cell lung carcinoma cells were purchased from American Type Culture Collection (Manassas VA), used at a passage number below 20, and cultured in RPMI 1640 growth medium (Corning Life Sciences—Mediatech, Manassas VA USA) with 1% Penicillin/Streptomycin, 0.1% Gentamicin (Life Technologies, Eugene OR USA), and 10% FBS in 5% CO₂ at 37°C. U87 glioma cells were purchased from American Type Culture Collection (Manassas VA USA), used at a passage number below 20, and cultured in EMEM growth medium (Corning Life Sciences—Mediatech, Manassas VA USA) with 1% Penicillin/Streptomycin (Life Technologies, Eugene OR USA), 0.1% Gentamicin (Life Technologies, Eugene OR USA), and 10% FBS in 5% CO₂ at 37°C. Ferumoxytol (Feraheme^®) was obtained from AMAG Pharmaceuticals Inc., Cambridge, MA USA.

McConnell H.L., Schwartz D.L., Richardson B.E., Woltjer R.L., Muldoon L.L, & Neuwelt E.A. (2016). Ferumoxytol nanoparticle uptake in brain during acute neuroinflammation is cell-specific. Nanomedicine : nanotechnology, biology, and medicine, 12(6), 1535-1542.

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Nanoformulation for Targeted Glioblastoma Treatment

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DSPE-PEG2000 was purchased from Nippon Oil and Fats Co., Ltd., (Tokyo, Japan). Poloxamer 188 was purchased from BASF Aktiengesellschaft (Limburgerhof, Germany). Cholesterol and soy lecithin were purchased from Shanghai Youngsun Foods Co., Ltd. (Shanghai, China). Tween-80 was purchased from Shanghai Chemical Reagent Co., Ltd. (Shanghai, China). Glyceryl behenate (Compritol ATO 888) was purchased from Gattefosse S.A. (Saint-Priest, France). QUE was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (NICPBP; Beijing, China). TMZ was purchased from Jiangsu Tasly Diyi Pharmaceutical Co., Ltd. (Jiangsu, China). Annexin V and proridium iodide (PI) were obtained from BestBio Biotechnologies Co., Ltd. (Shanghai, China). RPMI-1640, penicillin-streptomycin, trypsin-ethylenediaminetetraacetic acid (EDTA) and fetal bovine serum (FBS) were obtained from Gibco BRL/Life Technologies (Carlsbad, CA, USA). U87 glioma cells were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA).

HU J., WANG J., WANG G., YAO Z, & DANG X. (2016). Pharmacokinetics and antitumor efficacy of DSPE-PEG2000 polymeric liposomes loaded with quercetin and temozolomide: Analysis of their effectiveness in enhancing the chemosensitization of drug-resistant glioma cells. International Journal of Molecular Medicine, 37(3), 690-702.

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