Fetal-bovine serum (FBS) and Dulbecco's modified Eagle's medium (DMEM) were procured from Atlanta Biologicals (Lawrenceville, GA) and Thermo Scientific (Logan, UT), respectively. Penstrep (50X) and trypsin-EDTA were obtained from Invitrogen (Carlsbad, CA). Silver nitrate, sodium borohydride, sodium citrate tribasic dehydrates, 2’, 7’-dichlorofluorescin diacetate (DCFH-DA), Zinc-oxide nanoparticles (ZnO-NPs) and titanium-dioxide nanoparticles (TiO2-NPs) were purchased from Sigma-Aldrich (St. Louis, MO). Silver nanoparticles (Ag-NPs) were synthesized and characterized as described earlier [18 (link)]. Countess cell counting chamber slides and trypan blue were obtained from Life technologies (Grand Island, NY) and Thermo Scientific, respectively. Tris buffered saline (TBS) and Tween 20 were from Boston Bioproducts (Ashland, MA). Cell proliferation reagent (WST-1) and DNAzol was procured from Roche Diagnostics (Mannheim, Germany) and Life Technologies, respectively. ECL plus detection kit was obtained from Thermo Scientific. Following antibodies were used: Cyclobutane pyrimidine dimer (Kamiya Biomedical Company, Seattle, WA), (6-4) photoproducts (Cosmo Bio Co. Ltd, Tokyo, Japan) and 8 hydroxyguanosine (Abcam, Cambridge, MA). All respective horseradish peroxidase-conjugated secondary antibodies were procured from Santa Cruz Biotechnology (Dallas, Texas).
Tween 20
Manufactured by Boston BioProducts
Sourced in United States
Tween 20 is a non-ionic surfactant commonly used in various laboratory applications. It is a polyoxyethylene sorbitan monolaurate, which has the ability to solubilize and emulsify certain compounds. Tween 20 is widely used in biochemical and molecular biology protocols to facilitate protein extraction, Western blotting, and enzyme-linked immunosorbent assays (ELISAs).
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions)
Cell proliferation reagent wst 1, by Roche (2 mentions)
Dulbecco s modified eagle medium dmem, by Corning (2 mentions)
Tris hcl, by Roche (2 mentions)
Dulbecco s phosphate buffered saline pbs, by Corning (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
Glycerol, by Thermo Fisher Scientific (2 mentions)
Iodoacetamide, by Merck Group (2 mentions)
Navo4, by Merck Group (2 mentions)
β glycerophosphate disodium, by Santa Cruz Biotechnology (2 mentions)
Nonidet p 40 substitute np 40, by Boston BioProducts (2 mentions)
Dithiothreitol (dtt), by Boston BioProducts (2 mentions)
Ethylenediamine tetraacetic acid edta, by Boston BioProducts (2 mentions)
Dulbecco s modified eagle s medium dmem, by Atlanta Biologicals (1 mentions)
Zinc oxide nanoparticles zno nps, by Merck Group (1 mentions)
Titanium dioxide nanoparticles tio2 nps, by Merck Group (1 mentions)
8 hydroxyguanosine, by Abcam (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
2 7 dichlorofluorescin diacetate dcfh da, by Merck Group (1 mentions)
Countess cell counting chamber slide, by Thermo Fisher Scientific (1 mentions)
4 protocols using tween 20
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Doxorubicin-Induced Apoptosis Assay
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The following reagents were used in this study: DMEM (Corning Incorporated, Corning, NY, USA); penicillin and streptomycin (10,000 U/mL each; GE Healthcare Life Sciences, Logan, UT, USA); FBS (Atlanta Biologicals, Lawrenceville, GA, USA); DOX hydrochloride (Tocris Bioscience, Bristol, UK); SuperSignal™ West Femto Maximum sensitivity substrate kit (Thermo Fisher Scientific, Waltham, MA, USA), Trypan blue (Corning Incorporated), cell proliferation reagent (WST-1) (Roche Diagnostic Corporation, Indianapolis, IN, USA), PE Annexin V Apoptosis Detection Kit (BD Biosciences, San Diego, CA, USA), Tris-buffered saline (Thermo Fisher Scientific), and Tween 20 (Boston Bioproducts, Ashland, MA, USA).
3
Chemical Reagents for Biochemical Assays
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The following chemicals were routinely used in this work: NaF (Sigma-Aldrich), NaVO4 (Sigma-Aldrich), β-Glycerophosphate disodium (Santa Cruz), Nonidet P-40 Substitute (NP-40) (Boston Bioproducts), iodoacetamide (Sigma-Aldrich), dithiothreitol (DTT) (Boston Bioproducts), Dulbecco’s Modified Eagle Medium (DMEM) (Corning), Dulbecco’s Phosphate Buffered Saline (PBS) (Corning), Tris-HCl (Roche), NaCl (Sigma-Aldrich), glycerol (Fisher Scientific), Ethylenediamine tetraacetic acid (EDTA) (Boston Bioproducts) and Tween-20 (Boston bioproducts).
4
Chemical Reagents for Biochemical Assays
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