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P mst1

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P-MST1 is a laboratory reagent used to detect and quantify phosphorylated MST1 (Mammalian Sterile 20-like kinase 1) in biological samples. MST1 is a serine/threonine protein kinase that plays a role in various cellular processes, including apoptosis, cell cycle regulation, and oxidative stress response. The phosphorylated form of MST1 is a key indicator of its activation and can provide insights into the regulation of these cellular pathways.

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Lab products found in correlation

Ecl kit, by Thermo Fisher Scientific (4 mentions) P yap, by Cell Signaling Technology (3 mentions) Gapdh, by Cell Signaling Technology (3 mentions) P lats1, by Cell Signaling Technology (2 mentions) Lats1, by Cell Signaling Technology (2 mentions) Peroxidase coupled secondary antibody, by Santa Cruz Biotechnology (2 mentions) Myc tag, by Cell Signaling Technology (2 mentions) Imaging system, by Bio-Rad (2 mentions) Catalase, by Abcam (2 mentions) Ab54073, by Abcam (2 mentions) Lats2, by Abcam (2 mentions) P foxo1, by Cell Signaling Technology (2 mentions) P enos, by Cell Signaling Technology (2 mentions) α tubulin, by Merck Group (2 mentions) Foxo3, by Cell Signaling Technology (2 mentions) Mnsod, by Merck Group (2 mentions) Foxo1, by Abcam (2 mentions) Histone h3, by Cell Signaling Technology (2 mentions) Lamin a c, by Cell Signaling Technology (2 mentions) Flag tag (flag), by Merck Group (2 mentions)

5 protocols using p mst1

1

Immunoblotting and Immunoprecipitation Assays

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Assays were performed as described previously.20 (link) WB analysis and IP were carried out with the following antibodies: FRMPD1 (Sigma, St. Louis, MO, USA, #SAB1407148, 1:500/WB); MYC-tag (Cell Signaling Technology Inc., Danvers, MA, USA, #2276, 1 µg/IP, 1:1,000/WB); GFP (Clontech, CA, USA, #JL-8, 1 μg/IP, 1:3,000/WB); GAPDH (Cell Signaling Technology, #5174, 1:1000/WB); MST1 (Cell Signaling Technology, #3682, 1:1,000/WB); p-MST1 (Cell Signaling Technology, #49332, 1:500/WB); LATS1 (Cell Signaling Technology, #9153, 1:1,000/WB); p-LATS1 (Cell Signaling Technology, #8654, 1:500/WB); YAP (Cell Signaling Technology, #4912, 1:1,000/WB); p-YAP (Cell Signaling Technology, #4911, 1:1,000/WB); LaminB1 (Abcam, Cambridge, MA, USA, #ab16048, 1:1,000/IB); Tubulin (Abcam, #ab52866, 1:1,000/IB). The peroxidase-coupled secondary antibodies were purchased from Santa Cruz Biotechnology Inc. (SantaCruz, CA, USA). Target proteins on PVDF membrane (EMD Millipore, Billerica, MA, USA) were visualized using an ECL kit (Thermo Fisher Scientific, Waltham, MA, USA) and images were obtained using the Bio-Rad Imaging System (Bio-Rad, Hercules, CA, USA).
Rong X., Han Q., Lin X., Kremerskothen J, & Wang E. (2019). FRMPD1 activates the Hippo pathway via interaction with WWC3 to suppress the proliferation and invasiveness of lung cancer cells. Cancer Management and Research, 11, 3395-3410.
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2

Immunoblotting Antibody Specifications

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Antibodies used for immunoblots were purchased from the indicated companies: Catalase (1:3000 dilution, #ab1877) (Abcam), p-Akt (Ser473) (1:2000 dilution, #9272), Akt (1:2000 dilution, #9271), p-eNOS (Ser1177) (1:1000 dilution, #9571), eNOS (1:1000 dilution, #9586), p-FoxO1 (Thr24, Ser256) (1:1000 dilution, #9464 and #9461), FoxO3 (1:1000 dilution, #9467), GAPDH (1:3000 dilution, #2118), Histone H3 (1:500 dilution, #9715), Lamin A/C (1:5000 dilution, #4777), p-Mst1 (Thr183) (1:1000 dilution, #3681), p-YAP (Ser127) (1:1000 dilution, #4911), YAP (1:2000 dilution, #4912) (Cell Signaling Technology), FoxO1 (1:2000 dilution, #1874-1) (epitomics), α-tubulin (1:5000 dilution, #T-6199), FLAG (1:2000 dilution, #F3165) (Sigma), MnSOD (1:3000 dilution, #611580), Mst1 (1:2000 dilution, #611052) (BD Biosciences), and Lats2 (1:1000 dilution, #ab54073 and #A300-479A) (Abcam and Bethyl Laboratories). The p-Lats2 (S872 and T1041) (1:500 dilution) antibodies were generated as described41 (link).
Shao D., Zhai P., Del Re D.P., Sciarretta S., Yabuta N., Nojima H., Lim D.S., Pan D, & Sadoshima J. (2014). A Functional Interaction between Hippo-YAP Signaling and FoxO1 Mediates the Oxidative Stress Response. Nature communications, 5, 3315.
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3

Splenic B Cell Activation Signaling Pathway

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Purified splenic B cells (2 × 106) were activated at 37°C with sAg for 5, 10 and 30 min. Cell lysates were obtained using a mixed RIPA buffer, as previously described.24 Lysates were run through SDS‐PAGE and western blotting. Abs from Cell Signaling Technology: BTK (8547S), pAKT (4060L), AKT (9272S), SHIP‐1 (2728S), pFOXO1 (9461S), FOXO1 (2880S), pS6 (4856S), S6 (2217S), pPI3K (4228S), PI3K (4292S), pMST1 (3681S), MST1 (PA5‐22015), pmTOR (5536S), mTOR (2983S), pEZRIN (3726S), STAT1 (14994S), P65 (4764S), pIKKB (2697S), IKKB (8943S), and anti‐human‐CCR2 (12199S). From Abcam: STAT5 (ab194898). From Santa Cruz Biotechnology: WASP (sc‐13139). Loading controls: anti‐mouse β‐actin (60008‐1‐IG‐10, Proteintech), anti‐human GAPDH (5174S, Cell Signaling Technology). Western blotting imaging was performed using the ChemiDoc™XRS + imaging systems (Bio‐Rad).
Zhu Y., Gu H., Yang L., Li N., Chen Q., Kang D., Lin S., Jing Y., Jiang P., Chen Q., Luo L., Liu J., Chang J., Li Z., Wang Y., Dai X., Miller H., Westerberg L.S., Park C., Kubo M., Gong Q., Dong L, & Liu C. (2022). Involvement of MST1/mTORC1/STAT1 activity in the regulation of B‐cell receptor signalling by chemokine receptor 2. Clinical and Translational Medicine, 12(7), e887.
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4

Immunoblotting Antibody Specifications

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Antibodies used for immunoblots were purchased from the indicated companies: Catalase (1:3000 dilution, #ab1877) (Abcam), p-Akt (Ser473) (1:2000 dilution, #9272), Akt (1:2000 dilution, #9271), p-eNOS (Ser1177) (1:1000 dilution, #9571), eNOS (1:1000 dilution, #9586), p-FoxO1 (Thr24, Ser256) (1:1000 dilution, #9464 and #9461), FoxO3 (1:1000 dilution, #9467), GAPDH (1:3000 dilution, #2118), Histone H3 (1:500 dilution, #9715), Lamin A/C (1:5000 dilution, #4777), p-Mst1 (Thr183) (1:1000 dilution, #3681), p-YAP (Ser127) (1:1000 dilution, #4911), YAP (1:2000 dilution, #4912) (Cell Signaling Technology), FoxO1 (1:2000 dilution, #1874-1) (epitomics), α-tubulin (1:5000 dilution, #T-6199), FLAG (1:2000 dilution, #F3165) (Sigma), MnSOD (1:3000 dilution, #611580), Mst1 (1:2000 dilution, #611052) (BD Biosciences), and Lats2 (1:1000 dilution, #ab54073 and #A300-479A) (Abcam and Bethyl Laboratories). The p-Lats2 (S872 and T1041) (1:500 dilution) antibodies were generated as described41 (link).
Shao D., Zhai P., Del Re D.P., Sciarretta S., Yabuta N., Nojima H., Lim D.S., Pan D, & Sadoshima J. (2014). A Functional Interaction between Hippo-YAP Signaling and FoxO1 Mediates the Oxidative Stress Response. Nature communications, 5, 3315.
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5

Western Blotting Analysis of Protein Targets

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Assays were performed as described previously.18 (link) Proteins were electrophoresed via sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis using an appropriate SDS concentration. For WB analysis, primary antibodies against the following target were obtained from Cell Signaling Technology, MYC tag (#2276, 1: 1000), Merlin (#12896, 1: 1000), p-Merlin (#73505, 1: 1000), MST1 (#3682, 1: 1000), p-MST1 (#49332, 1: 500), LATS1 (#9153, 1: 1000), p-LATS1 (#8654, 1: 500), YAP (#4912, 1: 1000), p-YAP (S127) (#4911, 1: 1000), β-catenin (#8480, 1: 1000), and Non-phospho (Active) β-catenin (#8814, 1: 1000). In addition, primary antibodies against PWP1 (#SC-390188, 1: 500), DVL2 (#SC-166303, 1: 500) were purchased from Santa Cruz Biotechnology, Inc. GAPDH (ZSGB-BIO, China, #TA309157, 1: 1000) was also used. Peroxidase-coupled secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. The rest of the antibodies were purchased from Cell Signaling Technology. The target proteins were transferred to a polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA) and visualized using an ECL Kit (Thermo Fisher Scientific, Waltham, MA, USA). Images were obtained using a Bio-Rad Imaging System (Bio-Rad Laboratories, Hercules, CA, USA).
Wei L., Li P., Luo Y., Zhang M., Yan T., Yang Y., Han Y., Liu S, & Wang E. (2020). PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin. OncoTargets and therapy, 13, 10025-10037.
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