Fuji dri chem 3030

Blood samples were centrifuged at 16,000 g for 2 min to obtain the plasma for the measurement of biochemical variables. Forty microliters of plasma was used to evaluate muscle injury and renal function at baseline (i.e., time 0) and at 1, 2, 4, 6 h after saline or LPS. The increases in plasma levels of CK and LDH were regarded as biochemical indicators of muscle injury. The elevation in plasma level of creatinine was considered as biochemical indicator of kidney function. All of these biochemical variables were analyzed by Fuji DRI-CHEM 3030 (Fuji Photo Film, Tokyo, Japan).

Blood samples were collected from a catheter placed in the carotid artery, and then were immediately centrifuged at 16,000 g for 2 min to obtain the serum for measuring biochemical variables. Sixty microliters of serum was used to analyze organ functions at baseline (i.e., time 0) and at 1, 2, 4, and 6 h. All of these biochemical variables were analyzed by Fuji DRI-CHEM 3030 (Fuji Photo Film, Tokyo, Japan). These enzymes measured in the serum were regarded as biochemical indicators of organ function. For instance, liver function was assessed by measuring the serum levels of ALT. Renal function was assessed by measuring the serum levels of CRE and BUN. In addition, LDH was measured to evaluate the extent of organ injury.

Whole-blood gas analysis was performed using an arterial blood gas analyzer (AVL OPTI Critical Care Analyzer; AVL Scientific Corp., Roswell, GA, United States). Whole blood was centrifuged at 16,000 × g for 2 min to obtain serum for measuring biochemical variables. The biochemical variables were analyzed using Fuji DRI-CHEM 3030 (Fuji Photo Film, Tokyo, Japan). A combination of both arterial blood gas and biochemical variables analyzers can estimate the parameters for arterial blood gas, biochemical data, and organ function/injury parameters, such as measurement of pulmonary function (PaO₂, SaO₂, oxygen content, oxygen capacity, and alveolar-arterial gradient); renal function (BUN, creatinine, bicarbonate, and base excess); liver function (GPT); and levels of hemoglobin, hematocrit, tissue perfusion (lactate), glucose, LDH, Na⁺, K⁺, Ca²⁺, and Mg²⁺.