Corneal TER was measured as previously described [16] (link). Briefly, a 1.0-mm-diameter Ag/AgCl electrode (Physiotech, Tokyo, Japan) was inserted into the anterior chamber through a small incision in the peripheral cornea, which had been made with an 18-gauge sharp needle (Terumo, Tokyo, Japan). Using biomedical adhesive (Alon-Alpha A; Sankyo), a 6.0-mm-internal diameter (0.28-cm2 inner area) nitrile rubber O-ring (Union Packing; SAN-EI, Osaka, Japan) was fixed on the cornea. Then, 60 μL of HBSS was placed inside the ring at the center of the cornea, and the other electrode was carefully placed on the cornea. The TER was measured using a volt-ohm meter (EVOMX, World precision Instruments, Saeasota, FL), which generates ±20-μA alternating current (AC) square wave current at 12.5 Hz, and data were recorded using a thermal arraycorder (WR 300-8; Graphtech, Tokyo, Japan).
18 gauge sharp needle
Manufactured by Terumo
Sourced in Japan
The 18-gauge sharp needle is a medical device designed for use in various laboratory and healthcare settings. It features a sharp, 18-gauge tip that is intended for precise fluid transfer or sample collection procedures. The needle's core function is to provide a reliable means of accurately introducing or extracting small volumes of fluids or samples.
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Lab products found in correlation
2 protocols using 18 gauge sharp needle
1
Corneal Transepithelial Electrical Resistance Measurement
2
In Vivo Corneal Transepithelial Resistance Measurement
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Rabbits were anesthetized using an intramuscular injection of 30 mg/kg ketamine (Ketalar, Daiichi Sankyo, Tokyo, Japan) and 5 mg/kg xylazine (Celactal, Bayer Yakuhin, Osaka, Japan). A 1.0 mm diameter custom-made Ag/AgCl electrode (Physiotech, Tokyo, Japan) was inserted into the anterior chamber, following a small incision made in the peripheral cornea using an 18-gauge sharp needle (Terumo, Tokyo, Japan). A 6.0 mm internal diameter (0.28 cm 2 inner area) nitrile rubber O-ring (Union Packing, SAN-EI, Osaka, Japan) was fixed on the cornea using biomedical adhesive (Alon-Alpha A, Daiichi Sankyo, Tokyo, Japan). Subsequently, 80 µL of HBSS were added in the ring, with a second electrode then placed in the HBSS on the cornea. The TER was measured in real time using a volt-ohm meter (EVOMX, World Precision Instruments, Sarasota, FL, USA) generating a ±20 μA AC square wave current at 12.5
Hz. The specific methodology and photographs of the in vivo corneal TER measurement system have been previously published [9] [10] [11] [12] (link). The sample size for the corneal TER study was set at 4 to 8, which we found to be sufficient for our statistical analyses in our previous TER studies [9] [10] [11] [12] (link).
Hz. The specific methodology and photographs of the in vivo corneal TER measurement system have been previously published [9] [10] [11] [12] (link). The sample size for the corneal TER study was set at 4 to 8, which we found to be sufficient for our statistical analyses in our previous TER studies [9] [10] [11] [12] (link).
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