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Anti fbw7

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Anti-FBW7 is a laboratory reagent that targets the FBW7 protein. FBW7 is an E3 ubiquitin ligase that plays a role in the regulation of cellular processes. The Anti-FBW7 reagent can be used to study the function and interactions of the FBW7 protein in a research setting.

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Lab products found in correlation

Nile red, by Merck Group (2 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Anti s6 ribosomal protein, by Cell Signaling Technology (2 mentions) Anti cleaved parp, by Cell Signaling Technology (2 mentions) Mg132, by Merck Group (2 mentions) Anti flag, by Merck Group (2 mentions) Anti acc, by Cell Signaling Technology (2 mentions) Anti 4e bp1, by Cell Signaling Technology (2 mentions) Anti ogt, by Merck Group (2 mentions) Anti o glcnac, by Merck Group (2 mentions) Anti fas, by Cell Signaling Technology (2 mentions) Anti psrebp1, by Merck Group (2 mentions) Anti praptor, by Cell Signaling Technology (2 mentions) Anti acly, by Abcam (2 mentions) Methyl pyruvate, by Merck Group (2 mentions) Compound c, by Selleck Chemicals (2 mentions) Anti ubiquitin, by Cell Signaling Technology (2 mentions) Dorsomorphin 2hcl, by Selleck Chemicals (2 mentions) Anti c myc, by Novus Biologicals (2 mentions) Anti srebp 1, by Novus Biologicals (2 mentions)

Spelling variants of this product

Rabbit Anti-FBW7 (3 citations) Anti-FBW7 (A301-720A) (2 citations) Anti-Fbw7 antibody (2 citations)

4 protocols using anti fbw7

1

Immunostaining of Tumor Markers in Clinical Samples

Cited 1 time
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The clinical tissue samples used in this study was same as our previous study, which were histopathologically and clinically diagnosed at Fudan University Shanghai Cancer Center from 2010 to 2011, and prior patient consent and approval from the Institutional Research Ethics Committee were obtained [5 (link)]. IHC staining was performed as described previously [20 ]. Anti-FBW7 (1:100, Bethyl), anti-NR4A1 (1:50, Abcam), anti-SCD1 (1:50, Cell Signaling Technology) were used to detect protein expression.
Ye Z., Zhuo Q., Hu Q., Xu X., Mengqi l.i.u., Zhang Z., Xu W., Liu W., Fan G., Qin Y., Yu X, & Ji S. (2020). FBW7-NRA41-SCD1 axis synchronously regulates apoptosis and ferroptosis in pancreatic cancer cells. Redox Biology, 38, 101807.
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2

Regulation of SREBP1 Signaling

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MG132, Nile Red, Methyl-pyruvate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Compound C and Dorsomorphin (2HCL) from Selleck-Chemicals (Houston, TX, USA). Ac-5sGlcNAc was provided by D. Vocadlo (Simon Fraser University). Antibodies used were Anti-OGT, Anti-O-GlcNAc, Anti-FLAG from Sigma-Aldrich; Anti-pSREBP1-(S372), Anti-pAMPK-(T172), Anti-pRaptor-(S792), Anti-pS6 Ribosomal Protein-(S240/244), Anti-p4EBP1-(T70), Anti-AMPK, Anti-Raptor, Anti-S6 Ribosomal Protein, Anti-4EBP1, Anti-FAS, Anti-ACC, Anti-Ubiquitin, Anti-Cleaved Caspase 3, Anti-Cleaved PARP from Cell Signaling (Danvers, MA, USA); Anti-Actin, Anti-Bcl2 from Santa Cruz Biotechnology; Anti-SREBP1, Anti-HIF1α, Anti-c-MYC from Novus Biologicals; Anti-SREBP1, Anti-ACLY, Anti-Glut1 from Abcam; Anti-FBW7 from Bethyl Labs. pLKO.FLAG-SREBP1 (Addgene-32017 from D. Sabatini).
Sodi V.L., Bacigalupa Z.A., Ferrer C.M., Lee J.V., Gocal W.A., Mukhopadhyay D., Wellen K.E., Ivan M, & Reginato M.J. (2017). Nutrient sensor O-GlcNAc transferase controls cancer lipid metabolism via SREBP-1 regulation. Oncogene, 37(7), 924-934.
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3

Regulation of SREBP1 Signaling

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MG132, Nile Red, Methyl-pyruvate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Compound C and Dorsomorphin (2HCL) from Selleck-Chemicals (Houston, TX, USA). Ac-5sGlcNAc was provided by D. Vocadlo (Simon Fraser University). Antibodies used were Anti-OGT, Anti-O-GlcNAc, Anti-FLAG from Sigma-Aldrich; Anti-pSREBP1-(S372), Anti-pAMPK-(T172), Anti-pRaptor-(S792), Anti-pS6 Ribosomal Protein-(S240/244), Anti-p4EBP1-(T70), Anti-AMPK, Anti-Raptor, Anti-S6 Ribosomal Protein, Anti-4EBP1, Anti-FAS, Anti-ACC, Anti-Ubiquitin, Anti-Cleaved Caspase 3, Anti-Cleaved PARP from Cell Signaling (Danvers, MA, USA); Anti-Actin, Anti-Bcl2 from Santa Cruz Biotechnology; Anti-SREBP1, Anti-HIF1α, Anti-c-MYC from Novus Biologicals; Anti-SREBP1, Anti-ACLY, Anti-Glut1 from Abcam; Anti-FBW7 from Bethyl Labs. pLKO.FLAG-SREBP1 (Addgene-32017 from D. Sabatini).
Sodi V.L., Bacigalupa Z.A., Ferrer C.M., Lee J.V., Gocal W.A., Mukhopadhyay D., Wellen K.E., Ivan M, & Reginato M.J. (2017). Nutrient sensor O-GlcNAc transferase controls cancer lipid metabolism via SREBP-1 regulation. Oncogene, 37(7), 924-934.
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4

Antibody Reagents for Signaling Pathways

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Anti-Akt (#4685), anti-phospho-Akt S473 (#4060), anti-phospho-Akt T308 (#9275), anti-Cyclin E (#4129), anti-GSK3β (#9315), anti-phospho-GSK3β (#9336), anti-NDRG1 (# 5196), anti-phospho-NDRG1 (#3217), anti-p110α (#4249), anti-S6K (#2705), anti-pS6K T389 (#9205), anti-SGK3 (#8156), anti-Vps34 (#4263) and anti-β-actin (#4970) were purchased from Cell Signaling Technologies. Anti-FLAG M2 (#F3165) was from Sigma-Aldrich. anti-SGK3 pT320 (# S1010-85W8) was from US Biologicals. Anti-GFP (# sc-9996), anti-GST (# sc-459) and anti-INPP4B (#sc-12318) were from Santa Cruz. Anti-Fbw7 (#A301–720A) was from Bethyl Laboratories. anti-SGK3 (#LS-C132061) for immunoprecipitation was from LifeSpan BioSciences. Anti-HA was generated and purified from the 12CA5 hybridoma. Anti-p85, generated in house, has been described. Horseradish peroxidase-conjugated anti-goat was from Millipore. Horseradish peroxidase-conjugated anti-rabbit and anti-mouse immunoglobulin G antibodies were from Chemicon.
Gasser J.A., Inuzuka H., Lau A.W., Wei W., Beroukhim R, & Toker A. (2014). SGK3 mediates INPP4B-dependent PI 3-Kinase signaling in breast cancer. Molecular cell, 56(4), 595-607.
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