Hexane

Manufactured by Biosolve

Sourced in France

Hexane is a colorless, flammable liquid hydrocarbon that is used as a solvent in various industrial applications. It has the chemical formula C6H14 and is commonly used in the extraction and purification of oils, fats, and other substances.

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Lab products found in correlation

Methanol, by Biosolve (3 mentions) Acetone, by Biosolve (3 mentions) 17β estradiol, by Merck Group (2 mentions) Sodium pyruvate, by Merck Group (2 mentions) Lysis reagent, by Promega (2 mentions) Dimethyl sulfoxide (dmso), by Biosolve (2 mentions) Ultra pure ethanol, by Merck Group (2 mentions) Dulbecco s modification of eagle s medium high glucose dmem without phenol red, by Merck Group (2 mentions) Glass fiber filters mg 227 1 60, by Sartorius (2 mentions) Luciferine reagent, by Promega (2 mentions) Acetonitrile, by Biosolve (2 mentions) Abil em 90, by Evonik (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Milli q water purification system, by Merck Group (1 mentions) Formic acid, by Biosolve (1 mentions) Acetonitrile acn, by Biosolve (1 mentions) Methanol, by Merck Group (1 mentions) Ammonium molybdate, by Merck Group (1 mentions) Boron trifluoride diethyl etherate, by Merck Group (1 mentions) Chloroform, by Biosolve (1 mentions)

Spelling variants of this product

N-hexane (7 citations) Solvents hexane (2 citations)

7 protocols using hexane

Cytotoxicity Evaluation of Doxorubicin Formulations

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DOX.HCl was purchased from Guanyu bio-technology Co., LTD (Xi’an, China). Bovine β-gus (G0376, Type B-3, 2000 units/mg solid) and 4-methylumbelliferyl β-d-glucuronide (M9130, 4-MUG) were purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands). Irgacure 2959 was obtained from Ciba Specialty Chemicals Inc. (Bazel, Switzerland). ABIL EM 90 was provided from Evonik Industries AG (Essen, Germany). Acetonitrile (ACN), dichloromethane (DCM), dimethylformamide (DMF), ethyl acetate, methanol, hexane, and dimethyl sulfoxide (DMSO) were obtained from Biosolve (Valkenswaard, The Netherlands). RPMI 1640 (R8758) and fetal bovine serum (FBS) were purchased from ThermoFisher (Bleiswijk, The Netherlands). CellTiter 96^® AQueous One Solution Cell Proliferation Assay (G3580, MTS reagent) was obtained by Promega (Leiden, The Netherlands). All other chemicals and reagents were obtained from Sigma-Aldrich (Zwijndrecht, The Netherlands).

Besse H.C., Chen Y., Scheeren H.W., Metselaar J.M., Lammers T., Moonen C.T., Hennink W.E, & Deckers R. (2020). A Doxorubicin-Glucuronide Prodrug Released from Nanogels Activated by High-Intensity Focused Ultrasound Liberated β-Glucuronidase. Pharmaceutics, 12(6), 536.

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Estrogen and Androgen Receptor Bioassays

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Dimethylsulfoxide (DMSO) (minimum 99.7%), hexane (minimum 96%, suitable for CALUX), acetonitrile (minimum 99.99%) and acetone (minimum 99.95%) were purchased from Biosolve (The Netherlands). The estrogen standard 17β-estradiol (minimum 98%), ultra-pure ethanol, Dulbecco’s Modification of Eagle’s Medium high glucose (DMEM without phenol red) and sodium pyruvate (100 mM, sterile-filtered) were obtained from Sigma-Aldrich (Belgium). Minimum Essential Medium Alpha (α-MEM), penicillin-streptomycin, Fetal Bovine Serum (FBS), FBS charcoal-stripped, L-glutamine (200 mM), trypsine, trypsine without phenol red and Phosphate-Buffered Saline (PBS) were obtained from Life Science Technology (United Kingdom). Glass fiber filters MG 227/1/60 with a diameter of 150 mm were purchased from Sartorius (Belgium). Luciferine reagent and lysis reagent were obtained from Promega (The Netherlands). The recombinant, estrogen-responsive BG1Luc4E2 ovarian carcinoma and the androgen-responsive T47D human breast carcinoma cell lines were previously described (Rogers and Denison, 2000 (link); Rogers and Denison, 2002 (link)).

Croes K., Van den Heuvel R., Van den Bril B., Staelens J., Denison M.S., Van Langenhove K., Vandermarken T, & Elskens M. (2016). Assessment of estrogenic and androgenic activity in PM10 air samples from an urban, industrial and rural area in Flanders (Belgium) using the CALUX bioassay. Environmental research, 150, 66-72.

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Comparison of Insect Larvae Processing

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Hermetia illucens larvae were kindly provided by the Laboratory of Entomology (Wageningen University, The Netherlands). H. illucens larvae were washed and dried before freezing. Tenebrio molitor larvae were purchased from Insectenkwekerij Van de Ven (Deurne, The Netherlands). Alphitobius diaperinus larvae were purchased from Kreca Ento-Feed BV (Ermelo, The Netherlands). Larvae were frozen with liquid nitrogen and stored at −22 °C.
Ultra-high-performance liquid chromatography/mass spectrometry (UHPLC-MS) grade formic acid, methanol, hexane, acetonitrile (ACN) and water were purchased from Biosolve BV (Valkenswaard, The Netherlands). Ethylenediaminetetraacetic acid (EDTA) was purchased from Arcros organics (New Jersey, USA).
All other chemicals used were purchased from Sigma Aldrich (St. Louis, USA) or Merck Millipore (Billerica, USA). Water was acquired using a Milli-Q water purification system (Millipore, Billerica, USA). Ferric iron was used as FeCl₃ (purity ≥ 99.0%), unless stated otherwise. Tyrosinase originated from Agaricus bisporus, which was purified to 3000 U/mL as described before^{31 (link)}.

Janssen R.H., Canelli G., Sanders M.G., Bakx E.J., Lakemond C.M., Fogliano V, & Vincken J.P. (2019). Iron-polyphenol complexes cause blackening upon grinding Hermetia illucens (black soldier fly) larvae. Scientific Reports, 9, 2967.

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Extraction and Analysis of ORURO Chia Seeds

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We chose to study the ORURO variety, which is a new genotype bred in France in 2017, adapted to the climatic conditions of the region [34 (link)]. ORURO chia seeds (Salvia hispanica L.) were purchased from the producer Agrofün, Occitania, France.
Purified rapeseed lecithin (Solae Europe SA society, Geneva, Switzerland), rapeseeds (NATUR-Haus Jurgen Kaiser, NK-Wiebelskirchen, Germany) and flax seeds (Linum usitatissimum L.) (Graines de Folie, La E Penell, Normandy, France) were used as for oil extractions (seeds) or NL preparation (rapeseed lecithin).
Ammonium molybdate, internal standard nonadecanoic acid (C19), boron trifluoride diethyl etherate and methanol were purchased from Sigma Aldrich (Saint-Quentin Fallavier, France). Formic acid was obtained from Fluka (Charlotte, NC, USA). Chloroform, hexane, diethyl and petroleum ethers were purchased from Biosolve (Dieuze, France).

Kuznetcova D.V., Linder M., Jeandel C., Paris C., Desor F., Baranenko D.A., Nadtochii L.A., Arab-Tehrany E, & Yen F.T. (2020). Nanoliposomes and Nanoemulsions Based on Chia Seed Lipids: Preparation and Characterization. International Journal of Molecular Sciences, 21(23), 9079.

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Estrogen Receptor Activation Assay

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Dimethylsulfoxide (DMSO) (minimum 99.7%), hexane (minimum 96%, suitable for CALUX), acetonitrile (minimum 99.99%) and acetone (minimum 99.95%) were purchased from Biosolve (The Netherlands). The estrogen standard 17β-estradiol (minimum 98%), ultra-pure ethanol, Dulbecco’s Modification of Eagle’s Medium high glucose (DMEM without phenol red) and sodium pyruvate (100 mM, sterile-filtered) were obtained from Sigma-Aldrich (Belgium). Minimum Essential Medium Alpha (α-MEM), penicillin-streptomycin, Fetal Bovine Serum (FBS), FBS charcoal-stripped, L-glutamine (200 mM), trypsine, trypsine without phenol red and Phosphate-Buffered Saline (PBS) were obtained from Life Science Technology (United Kingdom). Glass fiber filters MG 227/1/60 with a diameter of 150 mm were purchased from Sartorius (Belgium). Luciferine reagent and lysis reagent were obtained from Promega (The Netherlands). The recombinant, estrogen-responsive BG1Luc4E2 ovarian carcinoma cell line was previously described (Rogers and Denison, 2000 (link)).

Croes K., Debaillie P., Van den Bril B., Staelens J., Vandermarken T., Van Langenhove K., Denison M.S., Leermakers M, & Elskens M. (2015). Assessment of estrogenic activity in PM10 air samples with the ERE-CALUX bioassay: method optimization and implementation at an urban location in Flanders (Belgium). Chemosphere, 144, 392-398.

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LC-MS Analysis of Steroid Hormones

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Methanol, hexane and isopropyl-alcohol (IPA) (LC-MS grade) were purchased from Biosolve (Valkenswaard, The Netherlands), CO2 was purchased from Air Liquide (Paris, France). Testosterone (>99%), progesterone (>99%) and -estradiol (>98%) were purchased from Sigma-Aldrich (Overijse, Belgium). The analytes were dissolved at a concentration of 100 µg/mL in pure MeOH. For the measurements where also very low injection volumes were investigated, a concentration of 500 µg/ml was used to obtain high enough UV signals. Some experiments were also performed with a 15/15/70 v%/v%/v% MeOH/IPA/hexane mixture as sample solvent, where the IPA was added to the mixture to allow better mixing of MeOH and hexane [12] . Since the improvement in performance with this sample solvent mixture is in agreement with earlier work [12, 13] and are thus not specific for the feed injector, these results are not discussed in detail. A Zorbax RX-SIL 4.6 x 150 mm with 5 µm particles was used for all experiments (Agilent Technologies, Waldbronn, Germany).

Vanderlinden K., Desmet G, & Broeckhoven K. (2020). Effect of the feed injection method on band broadening in analytical supercritical fluid chromatography. Journal of chromatography. A, 1630.

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Organic Solvent Extraction Protocol

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For analysis and organic extraction, dichloromethane DCM (Biosolve), methanol (Carlo Erba), acetone (Biosolve), and hexane (Biosolve) of HPLC grade were used.
Sodium azide (NaN3, 99.0% Sigma Aldrich) was used as biocide (200 mg/L).
Deionized (DI) water was produced with a Pure-Lab Option system from Elga.

Johansson C., Bataillard P., Biache C., Lorgeoux C., Colombano S., Joubert A., Pigot T, & Faure P. (2020). Ferrate^VI oxidation of polycyclic aromatic compounds (PAHs and polar PACs) on DNAPL-spiked sand: degradation efficiency and oxygenated by-product formation compared to conventional oxidants. Environmental science and pollution research international, 27(1).

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