Biofilms were observed using Leica SPE-II confocal microscopy (Leica) by washing the coupons with PBS and staining the cells with SYTO9 (100 µM) and PI (100 µM) with 5 min incubation in the dark. Biofilms were observed using Leica SPE-II confocal microscopy (Leica) under 60× magnification and the images were obtained with a Leica DFC500 camera and Leica LAS AF software.
Leica spe 2 confocal microscopy
Manufactured by Leica camera
The Leica SPE-II is a confocal microscopy system designed for high-resolution imaging of samples. It utilizes laser excitation and a pinhole aperture to obtain optical sections, allowing for the capture of three-dimensional data. The system is capable of producing detailed, high-quality images suitable for a variety of scientific and research applications.
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Lab products found in correlation
1 protocol using leica spe 2 confocal microscopy
1
Biofilm Imaging via Confocal Microscopy
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