Tough tg 5 camera

Material from the following collections has been studied or is deposited therein, introducing the abbreviations: National Biodiversity Center, Thimphu, Bhutan (NBCB), Naturalis Biodiversity Center, Leiden, the Netherlands (NBC), and Zoological Institute in St Petersburg, Russia (ZIN). Male genitalia were removed and macerated in an aqueous 10% KOH solution at ambient temperatures for 12–24 hours and stored in glycerol. Photos of the terminalia were taken through a Bresser Biolux NV microscope with a MicrOculair and CamLabLite software, and subsequently stacked using Combine ZP 1.0 software. The remaining photos were made using an Olympus Tough TG-5 camera with built-in focus stacking software. The male holotype and the female paratype of Eumerusdruk Smit sp. nov. had one leg removed for DNA barcoding (Hebert et al. 2003a (link), 2003b (link)). DNA barcodes, sequences and collection data were uploaded to the Barcode of Life Database (BOLD: http://www.boldsystems.org). Specimens are linked through their specimen code to their respective entry on BOLD. Terminology of morphological characters follows Thompson (1999) , with the exception of the terminology for the genitalia, which follows Doczkal (1996) and Hurkmans (1993) . Abdominal tergites and sternites are abbreviated with a ‘t’ or ‘s’ respectively.

All photographs used for color quantification were taken with an Olympus Tough TG-5 camera (Olympus, China) in the microscope mode against the same white background. One blue and one orange individual were included in each photograph to compensate for any unnoticed effects of poor color balance. The photograph was loaded into the GIMP software (https://gimp.org), and white balance was corrected against the white background with the Levels tool. Red, blue and green color values were recorded with the Color Picker Tool in GIMP from the pereon (approx. 6th segment), gut (the best visible segment), pereopods, and antennae (whichever was the most clearly visible). An example is shown in Fig. S2. The R/B ratio was subsequently used as a color index, similarly to how it was applied to study color morphs of the coconut crab (Nokelainen, Stevens & Caro, 2017 (link)).
Some photographs were also taken with the Altami SPM0880 stereo microscope (Altami, Russia) equipped with a camera (U3CMOS05100KPA, Altami, Russia); the white balance was auto-corrected in the Altami Studio software prior to shooting against a 17% grey paper.