SA, APZ, and imipramine (IMP) were purchased from Sigma–Aldrich (St Louis, MO, USA). Acetonitrile and methanol (UPLC grade) were obtained from Panreac Chemicals (Barcelona, Spain), while potassium dihydrogen phosphate was procured from Winlab Ltd. (Maidenhead, Berkshire, UK). Milli-Q water was prepared in the laboratory by a purification system (Millipore Corp., Billerica, MA, USA). Anti-CYP3A2 (LS-C36108), CYP2D6 (PA1397) and anti β-Actin (sc-47778) antibodies were purchased from Lifespan Biosciences, Inc. (Seattle, WA, USA), Boster Bio, (Pleasanton, CA, USA) and Santa Cruz Biotechnology, Inc. (Dallas, Texas U.S.A), respectively. HRP-linked secondary antibodies were procured from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), and Luminata Forte Western HRP substrate was obtained from Millipore (Billerica, MA). All other chemical reagents used were of an analytical grade.
Imipramine imp
Manufactured by Merck Group
Sourced in United States, Germany
Imipramine (IMP) is a chemical compound used as a laboratory reagent. It is primarily utilized in research and scientific experimentation, particularly in the fields of biochemistry and pharmacology. Imipramine serves as a tool for studying various biological processes and mechanisms.
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Lab products found in correlation
3 protocols using imipramine imp
1
Quantitative Western Blotting of Metabolic Enzymes
2
Inhibition of CSF1R and HMGB1 Signaling
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The colony‐stimulating factor 1 receptor (CSF1R) inhibitor PLX3397 was obtained from MedChemExpress (Monmouth Junction, USA). Imipramine (IMP) was purchased from Sigma (St. Louis, MO). Wortmannin (WORT) was obtained from Calbiochem (USA). The HMGB1 ELISA was obtained from Immuno‐Biological Laboratories Co. International (Hamburg, Germany).
3
Zebrafish Anxiety Behavior Assessment
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The NTT is a specific test used for assessing anxiety in zebrafish, as described by Cachat et al. [54 ]. The trapezoidal tank (1.5 L) used measured 15.2 height × 27.9 top × 22.5 bottom × 7.1 width cm, equally divided into two horizontal sections (top and bottom). After 1 h of CsAE treatment, the animals were placed individually within the test tank without acclimatization, and swimming behavior was recorded for 6 min. The animals were recorded with a Logitech HD Webcam C922 Pro Stream camera (Logitech, Lausanne, Switzerland) placed 30 cm away from the tank, and the videos were analyzed using ANY-Maze® software (Stoelting CO, Wood Dale, IL, USA). The following parameters were registered: total distance in the tank (m) (to assess the locomotor activity), for the anxiety-like behavior: time spent in the top zone of the tank (s), and time spent in the bottom zone of the tank (s) were recorded. Representative tracking images of zebrafish locomotor activity from each group were obtained at the end of the analysis with ANY-Maze® software. Imipramine (IMP, 20 mg/L, Sigma-Aldrich, Darmstadt, Germany) was used as the positive control in the NTT test.
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