Quantification of the number of circulating hemocytes in EJS tumor-bearing larvae followed a previously established protocol (Petraki et al., 2015 ). Images were taken using a Leica florescence microscope (MZ10F) with an Adapt-spot camera and the Leica Application Suite software (v4.5). Post-acquisition image processing and analysis were performed using Fiji (Schindelin et al., 2012 (link)).
For immunohistochemistry, after circulating hemocytes in from EJS tumor-bearing larvae were bled onto microscope slides as described previously (Petraki et al., 2015 ), samples were fixed, washed, stained, and mounted as described in the immunohistochemistry and TUNEL sections above, using droplets of solutions on top of the samples. Slides were kept in moist chambers (damp tissues in small containers) to prevent droplets from drying out. Samples were imaged immediately.
For immunohistochemistry, after circulating hemocytes in from EJS tumor-bearing larvae were bled onto microscope slides as described previously (Petraki et al., 2015 ), samples were fixed, washed, stained, and mounted as described in the immunohistochemistry and TUNEL sections above, using droplets of solutions on top of the samples. Slides were kept in moist chambers (damp tissues in small containers) to prevent droplets from drying out. Samples were imaged immediately.