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3 protocols using verbenol

1

Oxidation of α-Pinene Using TS-1 Catalysts

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In the tested process, the following reagents were used for oxidation carried out with the use of titanium-silicate TS-1 catalysts: α-pinene (98%, Sigma Aldrich, Poznań, Poland), and oxygen (99.99%, Messer, Szczecin, Poland). Moreover, the following compounds were used as standards for chromatographic analyzes: α-pinene oxide (97%, Sigma Aldrich, Poznań, Poland), verbenol (95%, Sigma Aldrich, Poznań, Poland), verbenone (≥93%, Sigma Aldrich, Poznań, Poland), trans-pinocarveol (≥96%, Sigma Aldrich, Poznań, Poland), myrtenal (98%, Sigma Aldrich, Poznań, Poland), myrtenol (95%, Sigma Aldrich, Poznań, Poland), carveol (≥95%, Sigma Aldrich, Poznań, Poland), carvone (98%, Sigma Aldrich, Poznań, Poland), pinanediol (99%, Sigma Aldrich, Poznań, Poland). Campholenic aldehyde was identified by GC-MS method.
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2

Terpenoid Standards Characterization Protocol

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Terpenoid standards used in the study and their purity percentages as obtained from Sigma-Aldrich (UK) were: alloaromadendrene (-) (98%), camphene (95%), camphor (R) (95%), caryophyllene (-) (trans) (98.5%), 1,8-cineole, citral (95%), p-cymene (99%), geraniol (98%), geranyl acetate (97%), ledene (+) (95%), limonene (R) (+) (97%), linalool (±) (95%), linalyl acetate (97%), 6-methyl-5-hepten-2-one (96%), myrcene (90%), α-pinene (-) (99%), β-pinene (-) (99%), sabienene hydrate (97%), α-terpinene (85%), γ-terpinene (97%), terpinolene (95%), terpinen-4-ol (95%), α-terpineol (96%), terpinyl acetate (±) (90%) and verbenol (95%).
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3

Monoterpene Induction in Cell Cultures

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Cells were induced with the following chemicals: Camphor (Tokyo Chemical Industry, CAT#: C0011); borneol (Tokyo Chemical Industry, CAT#: B0525); fenchone (Tokyo Chemical Industry, CAT#: F0164); fenchol (Alfa Aesar, CAT#: L03211); eucalyptol (Acros Organics, CAT#: 110340050); verbenol (Sigma Aldrich, CAT#: 247065-5G); camphene (Tokyo Chemical Industry, CAT#: C0009); carvone (Acros Organics, CAT#: 154590050); alpha-pinene (Acros Organics, CAT#: 131270050); limonene (Tokyo Chemical Industry, CAT#: L0132); linalool (Combi-Blocks, CAT#: QH-3254). All monoterpenes were flushed with argon and stored at -20°C after opening. It should be noted that the commercially available camphene used in this study is >78% pure and contains as much as 20% tricyclene.
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