Human cd4 t cells

Manufactured by Miltenyi Biotec

Human CD4+ T cells are a type of T lymphocyte that express the CD4 surface glycoprotein. They play a central role in the adaptive immune response and are involved in the coordination of various immune functions.

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Lab products found in correlation

Retronectin, by Takara Bio (1 mentions) Facsaria 2, by BD (1 mentions) Anti relb c1e4, by Cell Signaling Technology (1 mentions) Odyssey imager, by LI COR (1 mentions) Irdye conjugated secondary antibody, by LI COR (1 mentions) Mg132, by Merck Group (1 mentions)

Close spelling variants of this product

Human CD4+ T cells isolation kit CD4+ T cells

2 protocols using human cd4 t cells

Isolation and Expansion of Naive T Cells and Tregs

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For the preparation of naive T cells (CD4⁺CD25⁻CD127⁺CD45RA⁺) and Tregs (CD4⁺CD25^hiCD127^lo), peripheral blood mononuclear cells (PBMCs) were separated using FACS sorter (FACSAria II, Becton Dickinson) from positively MACS-enriched human CD4⁺ T cells (Miltenyi Biotech). Native T cells and Tregs were stored in liquid nitrogen until use. Naïve T cells or Tregs (1×10⁶) were pre-stimulated for 48 hrs with anti-CD3ε (5 µg/ml) and anti-CD28 (1 µg/ml) antibodies in RPMI1640 media complemented with 10% Fetal bovine serum (FBS) and recombinant IL-2 (rIL-2, 200 IU/ml). ODNps25 (2 µM) were added in a case of required condition. To generate 17195 Tregs, Tregs were spin-fected (1500 × g, 2 hr, 32° C) with retroviral 17195 TCR in retronectin (10 µg/ml, Clontech) plate. The transduced cells were further expanded with rIL-2 for 9 to14 days.

Kim Y.C., Kim K.K., Yoon J., Scott D.W, & Shevach E.M. (2018). SAMHD1 post-transcriptionally controls the expression of Foxp3 and Helios in Human T regulatory Cells. Journal of immunology (Baltimore, Md. : 1950), 201(6), 1671-1680.

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Investigating CYLD and RELB Regulation

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Purified Human CD4+ T cells (Miltenyi Biotech) were pre-incubated with 10 μM proteasome inhibitor MG132 (SIGMA-Aldrich) and 10 μM Compound 2, 10 μM mepazine or 300 μM z-VRPR-fmk for 30 min prior stimulation with 10 ng/mL PMA and 250 ng/mL ionomycin. After 1 h stimulation, cells were lysed in RIPA buffer and protease inhibitor (cOmplete; Roche). Proteins were separated by SDS-polyacrylamide gel electrophoresis and electrotransferred to nitrocellulose. Membrane was blocked with 5% bovine serum albumin and probed with anti-CYLD (D1A10; Cell Signaling Technology) or anti-RELB (C1E4; Cell Signaling Technology). After incubation with IRDye conjugated secondary antibodies (LI-COR), proteins were detected using a Odyssey imager (LI-COR).

Dumont C., Sivars U., Andreasson T., Odqvist L., Mattsson J., DeMicco A., Pardali K., Johansson G., Yrlid L., Cox R.J., Seeliger F., Larsson M., Gehrmann U., Davis A.M, & Vaarala O. (2020). A MALT1 inhibitor suppresses human myeloid DC, effector T-cell and B-cell responses and retains Th1/regulatory T-cell homeostasis. PLoS ONE, 15(9), e0222548.

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