The following antibodies were used for western blotting: β-actin-horseradish peroxidase (HRP) (C4, sc-47778; Santa Cruz Biotechnology), CIAO1 (ab83088; Abcam), Flag-M2, clone M2 (F1804; Sigma-Aldrich), MIP18/FAM96B (20108-1-AP; Proteintech), MMS19 (16015-1-AP; Proteintech), c-myc (A00704-100; Genescript), polymerase delta catalytic subunit (ab10362; Abcam), and XPD (ab54676; Abcam).
Ab54676
Manufactured by Abcam
Ab54676 is a high-quality laboratory equipment product manufactured by Abcam. It is designed for specific laboratory applications, but a detailed description of its core function is not available at this time.
Automatically generated - may contain errors
3 protocols using ab54676
1
Western Blot Antibody Validation
2
Western Blot Antibody Validation
3
Quantifying DNA Damage Response in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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γH2AX signal in HeLa cells by FACS was measured using a commercial kit (FlowCellect Multi-Color DNA Damage Response Kit, Millipore, Germany) following the manufacturer's protocol. The methods for the analyses of yeast UV survival curves, cell cycle profiles, Pulsed-Field Gel Electrophoresis and spontaneous and UV-induced Rad52 foci have been previously described in detail [15] (link). Cell treatment, protein extraction and Western Blotting for Rad53 activation has been performed strictly as previously described [23] (link). Rad53 antibody used was previously described [38] (link). For XPD and XPA Western blots, 25 µg of total amount of protein, extracted from U2OS cells, were used. Antibodies XPD (abcam ab54676, 1∶5000), XPA (abcam ab2352, 1∶1000) and β-Actin (abcam ab8226, 1∶5000) diluted in TBS-Tween 0.1% with 5% milk were incubated overnight at 4°C.
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