Binding from serum assays were performed as previously described (Agarwal et al., 2013 (link)). Microtiter plates were coated overnight with SsPepO (0.25 μg/well) at 4°C. Casein was used as a control. After blocking for 2 h at 37°C, human serum was added to the wells for 1.5 h at 37°C. The samples were measured with rabbit anti-plasminogen antibody (Proteintech, Wuhan, China) followed by peroxidase-conjugated secondary antibody (Proteintech, Wuhan, China). The absorbance was measured at 490 nm.
Rabbit anti plasminogen antibody
Manufactured by Proteintech
Rabbit anti-plasminogen antibody is a laboratory reagent used to detect and quantify plasminogen, a key component of the fibrinolytic system. This antibody is produced in rabbits and specifically binds to and identifies plasminogen, allowing for its detection and measurement in various research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit anti plasminogen antibody
1
Serum Binding Assay for SsPepO
2
Binding of Strains to Plasminogen
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Strains binding to immobilized plasminogen were performed as described previously (Agarwal et al., 2013 (link)). Microtiter plates were incubated overnight with strains [107 colony-forming units (CFU)] at 37°C. After being blocked for 2 h, plasminogen was added to wells for 1.5 h. The samples were measured with rabbit anti-plasminogen antibody (Proteintech, Wuhan, China) followed by peroxidase-conjugated secondary antibody (Proteintech, Wuhan, China). The absorbance was measured at 490 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!