Log In Sign up
The largest database of trusted experimental protocols

Pcdna6 expression vector

Manufactured by Thermo Fisher Scientific
Visit Supplier

The pcDNA6 expression vector is a plasmid designed for the expression of recombinant proteins in mammalian cell lines. It contains a cytomegalovirus (CMV) promoter to drive high-level transgene expression, as well as a blasticidin resistance gene for selection of transfected cells.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

PcDNA6 vector (10 citations) PcDNA6.0 expression vector (4 citations) Expression vectors (2 citations) PcDNA6/V5-His A expression vector (2 citations)

2 protocols using pcdna6 expression vector

1

Cloning and Expression of DING p38SJ

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
DING p38SJ was cloned using RT-PCR and forward primers designed from the known DING p27SJ sequence and reverse primers from the Pseudomonas DING gene. Two fragments (1-788 and 788-1179) were then combined, and full-length cDNA was created to encode the 38 kDa DING p38SJ (364 amino-acids, GenBank #: AAW57408.2). DING p38SJ cDNA was then cloned into pcDNA6 expression vector (Invitrogen) [2 (link)].
Darbinian N., Darbinyan A., Merabova N., Gomberg R., Chabriere E., Simm M., Selzer M.E, & Amini S. (2020). DING Protein Inhibits Transcription of HIV-1 Gene through Suppression of Phosphorylation of NF-κB p65. Journal of HIV and AIDS, 6(1), 175.
+ Open protocol
+ Expand
2

Cloning and Expression of DING p38SJ

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
DING p38SJ was cloned using RT-PCR and forward primers designed from the known DING p27SJ sequence and reverse primers from the Pseudomonas DING gene. Two fragments (1-788 and 788-1179) were then combined, and full-length cDNA was created to encode the 38 kDa DING p38SJ (364 amino-acids, GenBank #: AAW57408.2). DING p38SJ cDNA was then cloned into pcDNA6 expression vector (Invitrogen) [2 (link)].
Darbinian N., Darbinyan A., Merabova N., Gomberg R., Chabriere E., Simm M., Selzer M.E, & Amini S. (2020). DING Protein Inhibits Transcription of HIV-1 Gene through Suppression of Phosphorylation of NF-κB p65. Journal of HIV and AIDS, 6(1), 175.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!