Formic acid and all solvents used were of the LC-MS grade. Ultrapure water (resistivity of 18.2 MΩ cm−1 at 25°C) obtained from a Millipore water purification system was used. Standards of phenolic acids (gallic acid, caffeic acid, quinic acid, p-coumaric acid, ferulic acid, and sinapic acid) and flavonoids (catechin, epicatechin, rutin, naringin, gallocatechin, and eryodictyol-7-rutinoside) were obtained from Sigma Chemicals Co. (Germany). Sodium carbonate, potassium ferric cyanide, iron (II) sulfate, aluminum chloride, and hydrochloric acid were obtained from Merck (South Africa), while 2,2-azino-bis (3-ethylben-zothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Folin-Ciocalteu's reagent, gallic acid, 2,5,7,8-tetramethylchroman carboxylic acid (Trolox), potassium persulfate, glacial acetic acid, ascorbic acid, methanol, and ethanol were sourced from Sigma-Aldrich (St. Louis, MO, USA). All other solvents and chemicals used were of the reagent grade and purchased from Sigma-Aldrich (Germany).
2 2 azino bis 3 ethylben zothiazoline 6 sulfonic acid diammonium salt abts 2 2 diphenyl 1 picrylhydrazyl dpph
Manufactured by Merck Group
Sourced in United States
2,2-azino-bis (3-ethylben-zothiazoline-6-sulfonic acid) diammonium salt (ABTS) is a chemical compound used as a redox indicator. 2,2-diphenyl-1-picrylhydrazyl (DPPH) is a stable free radical commonly used as a reagent to evaluate antioxidant activity.
Automatically generated - may contain errors
Lab products found in correlation
Gallocatechin, by Merck Group (1 mentions)
Quinic acid, by Merck Group (1 mentions)
Potassium ferric cyanide, by Merck Group (1 mentions)
Naringin, by Merck Group (1 mentions)
Water purification system, by Merck Group (1 mentions)
Folin ciocalteu s reagent, by Merck Group (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
Ferulic acid, by Merck Group (1 mentions)
P coumaric acid, by Merck Group (1 mentions)
Sinapic acid, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
Epicatechin, by Merck Group (1 mentions)
Ultrapure water, by Merck Group (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
2 protocols using 2 2 azino bis 3 ethylben zothiazoline 6 sulfonic acid diammonium salt abts 2 2 diphenyl 1 picrylhydrazyl dpph
1
Comprehensive Phytochemical Analysis Protocol
2
Polyphenol Extraction from P. lobata
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The dried stem powders of P. lobata were provided by Hanpoong Pharm. Co. Ltd. (Seoul, Korea). The standard of daidzin (purity ≥98%), MS-grade formic acid, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and IBMX were purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC-grade acetonitrile and water were obtained from Burdick & Jackson (Muskegon, MI, USA).
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