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2 protocols using montanox 80

1

Formulation and Antimicrobial Evaluation

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CHX base was purchased from Inresa (Bartenheim, France), Montanox 80 (polysorbate 80, hydrophilic surfactant) was given by Seppic (Castres, France), and Lutrol® F68 (poloxamer 188, molecular mass 7,680–9,510 g/mol, 81% polyoxyethylene units) was provided by BASF (Ludwigshafen, Germany). Labrafil® M1944CS (mixture of mono-, di-, and triglycerides and mono- and difatty esters of polyethylene glycol 300) and Plurol® Oléique CC 497 (polyglyceryl-6 dioleate) were provided by Gattefossé (St Priest, France). PCL (molecular mass ~80,000 Da) was purchased from Sigma-Aldrich (St Louis, MO, USA). Müller–Hinton agar plates and bacterial and fungal strains were provided by Nosoco Tech (Lyon, France).
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2

Formulation and Characterization of Antioxidant Compounds

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Ethyl acetate (EtOAc), methanol (MeOH), toluene, hexane, formic acid, diethylether, glacial acetic acid, petroleum ether, sulfuric acid 96% (H2SO4) and dimethylsulfoxide (DMSO) were purchased from Carlo Erba (Val de Reuil, France). Dimethyl carbonate (DMC), sodium alginate, (±)-α-tocophérol 96% (vitamin E), oleic acid, linoleic acid, palmitic acid, myristic acid, stearic acid, palmitoleic acid, γ-linolenic acid, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), menadione and glucose were purchased from Sigma Aldrich (Saint-Quentin Fallavier, France). Phosphoric acid 85% was purchased from Merck pro analysis (Darmstad, Germany). Labrafac®® WL 1349 was purchased from Gattefossé (Saint-Priest, France). Montane 80®® and Montanox 80®® were purchased from Seppic (Castres, France). Copper nitrate Cu(NO3)2 was purchased from Fisher Scientific SAS (Illkirch, France). Vanillin, acetic acid trihydrate 99+% were purchased from Acros Organics (Geel, Belgium). Water was purified using a Milli-Q system (Millipore Corporation, Bedford, MA, USA).
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