Yellow zein, coumarin-6 and all other chemicals that are not specifically mentioned below were purchased from Sigma-Aldrich (Poole, UK). Methoxy PEG succinimidyl carboxymethyl ester with a molecular weight (MW) of 5 kDa (mPEG-SCM-5K) and 10 kDa (mPEG-SCM-10K) were obtained from JenKem Technology (Plano, TX). Roswell Park Memorial Institute (RPMI) 1640 cell culture medium, fetal bovine serum (FBS), L-glutamine, penicillin-streptomycin and TrypLE® Express came from Life Technologies (Paisley, UK). Bioware® B16-F10-luc-G5 mouse melanoma cells expressing the firefly luciferase were purchased from Caliper Life Sciences (Hopkinton, MA). Vectashield® containing 4’,6-diamidino-2-phenylindole (DAPI) was from Vector Laboratories (Peterborough, UK). Ultrapure water (with a resistivity of 18.2 MΩ.cm at 25°C) was collected using a Milli-Q® Integral water purification system from Merck (Kenilworth, NJ).
Yellow zein
Manufactured by Merck Group
Sourced in United Kingdom
Yellow zein is a protein-based material derived from corn. It is used as a component in various lab equipment and supplies. Yellow zein possesses specific physical and chemical properties that make it suitable for certain applications in the laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Mpeg scm 10k, by JenKem Technology (2 mentions)
Tryple express, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Phosphate buffered saline tablet, by Merck Group (2 mentions)
Amphothericin b solution, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (2 mentions)
Glutamax 1, by Thermo Fisher Scientific (2 mentions)
Vectashield containing 4 6 diamidino 2 phenylindole dapi, by Vector Laboratories (1 mentions)
Milli q integral water purification system, by Merck Group (1 mentions)
Rpmi 1640 cell culture medium, by Thermo Fisher Scientific (1 mentions)
Coumarin 6, by Merck Group (1 mentions)
Oil red, by Merck Group (1 mentions)
Poloxamer 188 plx188, by BASF (1 mentions)
Tripsin edta, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Rhodamine b, by Merck Group (1 mentions)
1640 cell culture media, by Thermo Fisher Scientific (1 mentions)
Precision plus protein dual color standard, by Bio-Rad (1 mentions)
4 protocols using yellow zein
1
Formulation and Characterization of PEGylated Nanoparticles
2
Retinoic Acid Nanoparticle Formulation
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Yellow zein, sodium deoxycholate monohydrate (SD), all-trans-retinoic acid (ATRA), red oil, rhodamine B, 3-[4,5-dimethylthiazol-2-yl]-3,5-diphenyltetrazolium bromide salt (used for MTT-tests), phosphate buffered saline (PBS) tablets, dimethyl sulfoxide, and amphothericin B solution (250 μg/mL), were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Tween 80 (T80) was provided by Acef S.p.a. (Piacenza, Italy). Poloxamer 188 (PLX188) was purchased from BASF (Ludwigshafen, Germany). Ethanol was obtained from Carlo Erba SpA (Rodano [MI], Italy), while cellulose membrane Spectra/Por MWCO 50 kDA was obtained from Spectrum Laboratories Inc. (Eindhoven, the Netherlands).
For the in vitro studies, Dulbecco’s Modified Eagle’s Medium (DMEM) and Roswell Park Memorial Institute1640 enriched with Glutamax I, tripsin/EDTA, penicillin/streptomycin solution and fetal bovine serum (FBS) were obtained from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). A549 and K562 cells were purchased from the IRCCS Azienda Ospedaliera Universitaria San Martino – IST Istituto Nazionale per la Ricerca sul Cancro.
For the in vitro studies, Dulbecco’s Modified Eagle’s Medium (DMEM) and Roswell Park Memorial Institute1640 enriched with Glutamax I, tripsin/EDTA, penicillin/streptomycin solution and fetal bovine serum (FBS) were obtained from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). A549 and K562 cells were purchased from the IRCCS Azienda Ospedaliera Universitaria San Martino – IST Istituto Nazionale per la Ricerca sul Cancro.
3
Characterization of PEGylated Zein Nanoparticles
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Yellow zein, Nile red, and all other chemicals that are not specifically mentioned below were purchased from Sigma-Aldrich (Poole, UK). Methoxy PEG succinimidyl carboxymethyl ester with a MW of 5 kDa (mPEG-SCM-5000) and 10 kDa (mPEG-SCM-10K) were obtained from JenKem Technology (Plano, TX, USA). Dulbecco’s Modified Eagle Medium (DMEM) and Roswell Park Memorial Institute (RPMI) 1640 cell culture media, L-glutamine, fetal bovine serum (FBS), penicillin-streptomycin, and TrypLE® Express came from Life Technologies (Paisley, UK). 4–20% Mini-PROTEAN® TGXTM gels, Precision Plus ProteinTM Dual Color Standards, 10× TGS buffer, 2× Laemmli sample buffer, and Silver Stain PlusTM Kit were purchased from Bio-Rad (Watford, UK). Vectashield® mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) came from Vector Laboratories (Peterborough, UK). Bioware® B16-F10-luc-G5 mouse melanoma cells were purchased from Caliper Life Sciences (Hopkinton, MA, USA).
4
Zein-based Carrier Evaluation for Cancer
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Yellow zein, sodium deoxycholate monohydrate (SD), 3-[4,5-dimethylthiazol-2-yl]-3,5-diphenyltetrazolium bromide salt (used for MTT-tests), phosphate buffered saline (PBS) tablets, dimethyl sulfoxide, and amphothericin B solution (250 μg/ml) were all purchased from Sigma Aldrich (Milan, Italy). Paclitaxel (PTX) was kindly provided by Indena S.p.a. (Milan, Italy). [3H]cholesteryl hexadecyl ether ([3H]CHE, 40 Ci/mmoL) was obtained from Perkin Elmer-Italia (Monza, Italy).
For the in vitro studies, DMEM (Dulbecco's Modified Eagle's Medium) and RPMI1640 enriched with Glutamax I, tripsin/EDTA, penicillin/streptomycin solution and fetal bovine serum (FBS) were obtained from GIBCO (Life Technology, Milan, Italy). Human breast cancer cells (MCF-7) and chronic myelogenous leukemia (K562) were provided by the IRCCS Azienda Ospedaliera Universitaria San Martino – IST Istituto Nazionale per la Ricerca sul Cancro. All other materials and solvents used in this investigation were of analytical grade (Carlo Erba, Milan, Italy).
For the in vitro studies, DMEM (Dulbecco's Modified Eagle's Medium) and RPMI1640 enriched with Glutamax I, tripsin/EDTA, penicillin/streptomycin solution and fetal bovine serum (FBS) were obtained from GIBCO (Life Technology, Milan, Italy). Human breast cancer cells (MCF-7) and chronic myelogenous leukemia (K562) were provided by the IRCCS Azienda Ospedaliera Universitaria San Martino – IST Istituto Nazionale per la Ricerca sul Cancro. All other materials and solvents used in this investigation were of analytical grade (Carlo Erba, Milan, Italy).
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