Strains, plasmids and primers used in this study are listed in Tables S2-S3 respectively. nleF was amplified from EPEC E2348/69 and C. rodentium ICC169 genomic DNA by PCR. Site-directed mutagenesis was carried out by inverse PCR using KOD Hot Start polymerase and mismatch primers. All constructs were confirmed by sequencing (GATC biotech). For Western Blot, Mouse monoclonal anti-caspase-4 clone 4B9 (sc-56056; Santa Cruz), anti-α-Tubulin clone DM1A (T6199), mouse polyclonal antibody anti-caspase-11 p20 clone A-2 (sc-374615; Santa cruz) and anti-pro-IL-18 (CPTC-IL18-1; DSHB), the rabbit monoclonal anti-IL-18 (PM014; MBL), anti-caspase-5 (4429; Cell signalling) and the rabbit polyclonal antibody anti-GFP (Ab290; Abcam) were used as primary antibodies. Horse radish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Fc fragement; catalog no.111-035-008; Jackson immunoresearch) and HRP-conjugated goat anti-mouse IgG (Fc fragement; catalog no, 115-035-008; Jackson immunoresearch) were used as secondary antibodies.
Hrp conjugated goat anti rabbit igg fc fragement
Manufactured by Jackson ImmunoResearch
HRP)-conjugated goat anti-rabbit IgG (Fc fragement) is a secondary antibody conjugated with Horseradish Peroxidase (HRP). It is designed to detect and bind to the Fc region of rabbit immunoglobulin G (IgG) in immunoassay and immunohistochemistry applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti gfp ab290, by Abcam (2 mentions)
Anti caspase 4 clone 4b9, by Santa Cruz Biotechnology (2 mentions)
Anti α tubulin clone dm1a t6199, by Santa Cruz Biotechnology (2 mentions)
Anti caspase 11 p20 clone a 2, by Santa Cruz Biotechnology (2 mentions)
Hrp conjugated goat anti mouse igg fc fragement, by Jackson ImmunoResearch (2 mentions)
Sc 374615, by Santa Cruz Biotechnology (2 mentions)
Anti caspase 5, by Cell Signaling Technology (2 mentions)
2 protocols using hrp conjugated goat anti rabbit igg fc fragement
1
Molecular Cloning and Analysis
2
Molecular Cloning and Analysis
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Strains, plasmids and primers used in this study are listed in Tables S2-S3 respectively. nleF was amplified from EPEC E2348/69 and C. rodentium ICC169 genomic DNA by PCR. Site-directed mutagenesis was carried out by inverse PCR using KOD Hot Start polymerase and mismatch primers. All constructs were confirmed by sequencing (GATC biotech). For Western Blot, Mouse monoclonal anti-caspase-4 clone 4B9 (sc-56056; Santa Cruz), anti-α-Tubulin clone DM1A (T6199), mouse polyclonal antibody anti-caspase-11 p20 clone A-2 (sc-374615; Santa cruz) and anti-pro-IL-18 (CPTC-IL18-1; DSHB), the rabbit monoclonal anti-IL-18 (PM014; MBL), anti-caspase-5 (4429; Cell signalling) and the rabbit polyclonal antibody anti-GFP (Ab290; Abcam) were used as primary antibodies. Horse radish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Fc fragement; catalog no.111-035-008; Jackson immunoresearch) and HRP-conjugated goat anti-mouse IgG (Fc fragement; catalog no, 115-035-008; Jackson immunoresearch) were used as secondary antibodies.
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