The overnight cultures grown in LB medium at 37°C were diluted to the OD600 of 0.04 in the same medium, followed by a further dilution of 103-fold. The diluted samples (100 μL) were spread on LB agar plates and incubated at 37°C or 20°C. Colony images were acquired using CanoScan LiDE 700F. For macrocolony observation, overnight cultures were diluted to an OD600 of 0.02 in the same medium. Three microliters of the diluted cultures were spotted onto LB agar plates and incubated at 37°C or 20°C. The macrocolonies were photographed with a Canon EOS 5D Mark IV camera.
Eos 5d mark 4 camera
Manufactured by Canon
The Canon EOS 5D Mark IV is a high-performance digital single-lens reflex (DSLR) camera. It features a 30.4-megapixel full-frame CMOS sensor, DIGIC 6+ image processor, and 4K video recording capability.
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6 protocols using eos 5d mark 4 camera
1
Growth Characterization of Bacterial Colonies
2
Bacterial Motility Assay Protocols
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Swimming motility was determined on 0.3% LB agar (Merck, Shanghai, China) plates. We injected 0.5 µl of the standardized culture below the surface of the agar, and
incubated the plates overnight at 30°C. Twitch assays were conducted on 1% LB agar plates. We inoculated the bacteria by picking a colony from each LB plate that had been cultured overnight,
using a sterile tip. The tip was used to inoculate the bacteria at the bottom of each plate, which was then incubated at 37°C for 24 hr. We measured the zone of motility on each plate [3 (link)] and recorded it photographically using a Canon EOS 5D Mark IV camera.
incubated the plates overnight at 30°C. Twitch assays were conducted on 1% LB agar plates. We inoculated the bacteria by picking a colony from each LB plate that had been cultured overnight,
using a sterile tip. The tip was used to inoculate the bacteria at the bottom of each plate, which was then incubated at 37°C for 24 hr. We measured the zone of motility on each plate [3 (link)] and recorded it photographically using a Canon EOS 5D Mark IV camera.
3
Morphological Analysis of Insect Specimens
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Morphological terminology follows Anufriev and Emeljanov (1988) and Gnezdilov et al. (2014) . Photographs were taken using a Canon EOS 5D Mark IV camera with the lens Canon-MP-E-65mm f/2,8 1-5x Macro and the flash Canon Macro Twin-Lite MT-26EX-RT. Images were produced using Helicon Focus v. 7.6.4 and Adobe Photoshop СС 2019 software. The genital segments of male specimens examined were macerated in 10% potassium hydroxide (KOH) and figured in glycerine jelly (Brunel Micro Ltd, UK) using a Leica MZ9.5 stereomicroscope with a camera lucida. The map was prepared using Google Earth Pro (version 7.3) with map data sources as attributed in image.
The specimens examined are deposited in University of Delaware, Department of Entomology and Wildlife Ecology, Newark, Delaware, USA (UDCC ); Smithsonian Institution, National Museum of Natural History, Washington, DC (USNM ); Bernice P Bishop Museum of Natural History, Honolulu, Hawaii (BPBM ); and the Zoological Institute of the Russian Academy of Sciences, Saint Petersburg, Russian Federation (ZIN ).
The specimens examined are deposited in University of Delaware, Department of Entomology and Wildlife Ecology, Newark, Delaware, USA (
4
Discovering 'Living Fossil' Larvae in China
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The collection site is located at the Huagaoxi National Nature Reserve, Sichuan, China, which is famous for the largest areas of tree ferns (Alsophila spinulosa) known as "living fossil" in China and the Danxia landforms. The specimens were collected by picking larvae directly from the seeping rock surface with forceps, and then placed them into vials containing 90% ethanol in the field. Some specimens were dissected under the stereomicroscope and were mounted on slides with Hoyer's solution for examination under the microscope. Photographs were taken using a Canon EOS 5D Mark IV camera with MP-E 65 mm macro lens and the microscope with a digital camera attached. The holotype (mature male larva) and paratypes are deposited in the Insect Collection, South China Agricultural University (SCAU), Guangzhou, China. Material examined. Holotype: male mature larva (in ethanol, deposited in SCAU), CHINA, Sichuan Province, Xuyong County, Shuiwei Town, Huagaoxi National Nature Reserve, 20.v.2012, coll. Weifang Shi. Paratypes (in ethanol) : 10 larvae and 2 mature larvae on mounted slides same data as holotype.
5
Macro Photography of Genital Segments
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Photographs were taken using Canon EOS 5D Mark IV camera with the lens Canon-MP-E-65 mm f/2,8 1-5x Macro and the flash Canon Macro Twin-Lite MT-26EX-RT. Images were produced using Helicon Focus v. 7.6.4 and Adobe Photoshop CC 2019 software. The genital segments of male specimens studied were macerated in 10% KOH and figured in glycerin jelly (Brunel Micro Ltd, UK) using the Leica MZ 9.5 stereomicroscope with a camera lucida.
The specimens listed below including the type series of the new species described are deposited in the Zoological Institute of the Russian Academy of Sciences, Saint Petersburg, Russia (ZIN). The type specimens examined after the photos are from the Hungarian Natural History Museum, Budapest, Hungary (HNHM) and from the Finnish Museum of Natural History Luomus, Helsinki, Finland (FMNH: http://id.luomus.fi/GV.41537; http:// id.luomus.fi/GV.42323).
Label information for old and type material is quoted, with ' /' indicating new line and ' //' indicating next label.
The specimens listed below including the type series of the new species described are deposited in the Zoological Institute of the Russian Academy of Sciences, Saint Petersburg, Russia (ZIN). The type specimens examined after the photos are from the Hungarian Natural History Museum, Budapest, Hungary (HNHM) and from the Finnish Museum of Natural History Luomus, Helsinki, Finland (FMNH: http://id.luomus.fi/GV.41537; http:// id.luomus.fi/GV.42323).
Label information for old and type material is quoted, with ' /' indicating new line and ' //' indicating next label.
6
Aquatic Beetle Discovery in Western Ghats
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Specimens were collected from a small rock of pool habitat in the landslide hit area of Nelliyampathy forest range in Kundrachola region (515m, 10 0 30'58''N & 76 0 37'51"E) of southern Western Ghats (Image 1, 2). An aquarium hand net (Miller & Bergsten 2016) was used to collect the samples during the cool dry season from January to March 2019; a total of seven specimens were collected from the field and preserved in 80% ethanol prior to mounting. The holotype and paratype is deposited in the Department of Zoology, University of Calicut (DZUC). Specimens were imaged with a Canon EOS 5D Mark IV camera with MP-E 65mm lens, f/2.8 1-5X. Morphological terminology is according to Miller & Bergsten (2016) . Identification was done based on available literature and taxonomic keys (Regimbart 1899; Mukherjee & Sengupta 1986; Nilsson 2001; Miller & Bergsten 2014; Miller & Bergsten 2016) . Material examined: Holotype: DZUC/Dyt01/2020, 17.iii.2019, male, India: Kerala, Palakkad, southern Western Ghats, Nelliyampathy forest range-Kundrachola (10 0 30'58''N & 76 0 37'51"E), coll. P.P. Anand.
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