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Hacat cell line

Manufactured by Lonza
Sourced in Cameroon

The HaCaT cell line is a spontaneously immortalized human keratinocyte cell line derived from human skin. It is a well-characterized model for the study of keratinocyte biology and skin-related research.

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2 protocols using hacat cell line

1

Culturing Immortalized Human Keratinocytes

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The immortalized human epidermal keratinocyte (HaCaT) cell line was obtained from Lonza and Korean Cell Line Bank (Seoul, Korea). The HaCaT cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Biowest, Nuaille, France) supplemented with 10% fetal bovine serum (FBS, Biowest), and 1% penicillin and streptomycin (P/S, GIBCO, Carlsbad, CA, USA) at 37 °C in a humidified chamber with 5% CO2.
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2

Exosome-mediated TCDD/BaP effects in HaCaT cells

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The HaCaT cell line, an immortalized human keratinocyte cell line (Welgene, Daegu, South Korea), was cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Lonza, Walkersville, MD, United States) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Waltham, MA, United States) and 1% penicillin-streptomycin. Cultures were maintained at 37°C in a humidified CO2 incubator (5% CO2). HaCaT cells were treated with 10 nM 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) (Sigma–Aldrich) or 0.5 µM Benzo [a]pyrene (BaP) (Sigma–Aldrich) for 24 h. Exosomes were then isolated from TCDD- or BaP-treated HaCaT cells and utilized to stimulate recipient HaCaT cells at a concentration of 20 μg/mL for an additional 24 h.
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