N n n n tetramethylethylenediamine temed tris base

Manufactured by Merck Group

Sourced in China

N,N,N',N'-Tetramethylethylenediamine (TEMED) is a chemical compound commonly used as a polymerization catalyst in gel electrophoresis techniques. Tris base, also known as Trizma base, is a chemical compound that serves as a pH buffer in various laboratory applications.

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Tris base (352 citations) TEMED (168 citations) N,N,N′,N′-tetramethylethylenediamine (81 citations) N,N,N′,N′-tetramethylethylenediamine (TEMED) (52 citations) Tetramethylethylenediamine (46 citations) Tetramethylethylenediamine (TEMED) (17 citations) N′-tetramethylethylenediamine (4 citations)

2 protocols using n n n n tetramethylethylenediamine temed tris base

Crayfish Protein Analysis Protocol

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Fresh crayfish (P. clarkii) was purchased from the local market. Maleic acid (HPLC), ortho-phthalaldehyde (OPA, purity ≥ 98%) and DL-Serine (≥98%) from Macklin Co., Ltd. (Shanghai, China); N,N,N’,N’-Tetramethylethylenediamine (TEMED), Tris base (≥99.9%), β-mercaptoethanol (≥99%), and Coomassie brilliant blue R-250 from Sigma-Aldrich (St. Louis, MO, USA); acrylamide (≥99.5%), N,N-methylene bisacrylamide (≥99%), and Sodium dodecyl sulfate (SDS) from BioSharp Co., Ltd. (Shanghai, China); glycine and protein standards for SDS-PAGE from TAKARA Co., Ltd. (Tokyo, Japan); DL-dithiothreitol (DTT, purity ≥ 99%) and Coomassie brilliant blue G-250 from Fei Yang Biotechnology Co., Ltd. (Xi’an, China). All other reagents were of analytical grade.

Kang X., Ma M., Yuan J, & Huang Y. (2022). Characteristics and Mechanism of Crayfish Myofibril Protein Gel Deterioration Induced by Autoclaving. Foods, 11(7), 929.

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Molecular Mechanisms of DMBA-Induced Carcinogenesis

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DMBA (CAS # 57-97-6), sesame oil (CAS # 8008-74-0), 2-β-mercaptoethanol, 30% acrylamide/0.8% bisacrylamide, ammonium persulphate, glycerol, N′N′N′N′-Tetramethylethylenediamine (TEMED), Tris base, Tris HCL, Sodium chloride, Tween-20 were purchased from Sigma-Aldrich Inc. (St Louis, MO). RNeasy Mini kit, QIA shredder kit, RNeasy Min Elute kit, and Quantitect TM SYBR Green PCR kit were purchased from Qiagen Inc (Valencia, CA). All primers were purchased from the Iowa State University DNA facility. All primary antibodies were purchased from Abcam (Cambridge, MA). RNA later was obtained from Ambion Inc. (Austin, TX). Goat anti-rabbit secondary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Ponceau S was from Fisher Scientific. ECL plus chemical luminescence detection kit was obtained from GE Healthcare, Amersham (Buckinghamshire, UK).

Ganesan S., Nteeba J, & Keating A.F. (2014). Impact of obesity on 7,12-dimethylbenz[a]anthracene-induced altered ovarian connexin gap junction proteins in female mice. Toxicology and applied pharmacology, 282(1), 1-8.

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