2-Hydroxyethyl methacrylate (H), gelatin (G, Type A), ethylene glycol dimethacrylate (EGDMA), 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC), ammonium persulfate (APS), and N,N,N’,N’-tetramethylene diamine (TEMED) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Manuka honey (MHo) was supplied by Haines Apiaries Ltd. (ArtisanHoney®, Kaitaia, New Zealand). RPMI-1640 medium and supplements for cell proliferation, as well as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay components, were purchased from Sigma-Aldrich, St. Louis, MO, USA. All syntheses were performed in deionized water. All experiments were performed using lab-produced, ultra-distilled water.
N n n n tetramethylene diamine temed
Manufactured by Merck Group
Sourced in United States
N,N,N',N'-tetramethylene diamine (TEMED) is a chemical compound used as a catalyst in various laboratory applications. It is a clear, colorless liquid with a characteristic odor. TEMED is commonly used in polyacrylamide gel electrophoresis (PAGE) to facilitate the polymerization of acrylamide and bisacrylamide, forming the gel matrix. It plays a crucial role in the separation and analysis of biomolecules, such as proteins and nucleic acids.
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Lab products found in correlation
Rpmi 1640 medium and supplements for cell proliferation, by Merck Group (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt reduction assay components, by Merck Group (2 mentions)
Ammonium persulfate ap, by Merck Group (2 mentions)
N n methylenebisacrylamide bis, by Merck Group (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
P akt ser473, by Cell Signaling Technology (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
Annexin 5 fitc, by BD (1 mentions)
Fugene transfection reagent, by Promega (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Acrylamide, by Merck Group (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Sodium dodecyl sulphate (sds), by Merck Group (1 mentions)
Trizol, by Merck Group (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
Bromophenol blue, by Merck Group (1 mentions)
3 methyladenine, by Merck Group (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Cleaved caspase 9, by Santa Cruz Biotechnology (1 mentions)
P β cateninser552, by Cell Signaling Technology (1 mentions)
6 protocols using n n n n tetramethylene diamine temed
1
Gelatin-Based Hydrogel for Cell Proliferation
2
Synthesis of NIPAM Hydrogels
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Hydrogels were synthesized via free radical polymerization of N-isopropylacrylamide (NIPAM) (Sigma-Aldrich). N,N-Methylenebisacrylamide (BIS) (Sigma-Aldrich) was used as the crosslinking agent. Ammonium persulfate (APS) (Sigma-Aldrich) and N,N,N′,N′-tetramethylenediamine (TEMED) (Sigma-Aldrich) were employed as the initiator system. The reaction mechanism of hydrogel formation is described in the ESI (Scheme S1).†
3
Nimbolide Modulates PI3K/Akt/GSK-3β Signaling
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Acrylamide, AO, bovine serum albumin (BSA), bromophenol blue, CQ, 4,6-diamidino-2-phenylindol (DAPI), DMBA, ethidium bromide, JC-1 iodide, 3-methyladenine (3-MA), 2-mercaptoethanol, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), sodium dodecyl sulphate (SDS), N,N,N’,N’-tetramethylene diamine (TEMED) and Trizol were acquired from Sigma Chemical Company, St. Louis, MO, USA. Power SYBR® Green PCR master mix was obtained from Applied Biosystems, California, USA. Antibodies for Akt, β-actin, β-catenin, cleaved caspase-3, cleaved caspase-9, cytochrome c, GSK-3β, p-GSK-3βSer9, p-GSK-3βTyr216, PI3K, and Gapdh were purchased from Santa Cruz Biotechnology, USA. Antibodies for ATG5, Bax, Bcl-2, Beclin-1, Histone H2B, LC-3, p-AktSer473, p-β-cateninSer33,Ser37,Thr41, and p-β-cateninSer552 as well as ELISA kits were from Cell Signaling Technology, USA. Alexafluor-488 conjugated anti-rabbit antibody was obtained from Molecular Probes, Inc. (Eugene, OR, USA). Annexin V-FITC, propidium iodide (PI) kit and p62 antibody were purchased from BD Biosciences (San Diego, CA). Nimbolide was obtained from M/s Asthagiri Herbal Research Foundation, Chennai, India. FuGENE transfection reagent was procured from Promega. Oligonucleotide primers were purchased from Sigma Genosys, San Ramon, USA. All other reagents used were of analytical grade.
4
Hydrogel Scaffolds with Doped Hydroxyapatite
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2-Hydroxyethyl methacrylate (HEMA, Sigma-Aldrich, St. Louis, MO, USA), ethyleneglycol dimethacrylate (EGDMA) (Aldrich), potassium persulfate (PPS) (Fluka), and N, N, N′, N′-tetramethylene diamine (TEMED) (Sigma-Aldrich) were used as a monomeric component, crosslinking agent, initiator, and activator, respectively. Natural origin polymeric components, gelatin (type B from bovine skin) (Sigma-Aldrich), and sodium alginate (Sigma-Aldrich), as well as crosslinker 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) (Sigma-Aldrich) were used for hydrogel scaffolds syntheses. The deionized water was used as a solvent.
Materials used for the hydroxyapatite synthesis and doping were calcium nitrate pentahydrate (Ca(NO3)2 × 5H2O) (Sigma-Aldrich), magnesium nitrate hexahydrate (Mg(NO3)2 × 6H2O) (Sigma-Aldrich), strontium nitrate (Sr(NO3)2) (Sigma-Aldrich), gallium nitrate hydrate (Ga(NO3)3 × H2O) (Sigma-Aldrich), zinc nitrate hexahydrate (Zn(NO3)2 × 6H2O) (Sigma-Aldrich), ammonium dihydrogen phosphate (NH4H2PO4) (Sigma-Aldrich), and urea ((NH2)2CO) (Alfa Aesar). All chemicals and reagents were of analytical grade. All experiments were performed using lab-produced, ultra-distilled water.
Materials used for the hydroxyapatite synthesis and doping were calcium nitrate pentahydrate (Ca(NO3)2 × 5H2O) (Sigma-Aldrich), magnesium nitrate hexahydrate (Mg(NO3)2 × 6H2O) (Sigma-Aldrich), strontium nitrate (Sr(NO3)2) (Sigma-Aldrich), gallium nitrate hydrate (Ga(NO3)3 × H2O) (Sigma-Aldrich), zinc nitrate hexahydrate (Zn(NO3)2 × 6H2O) (Sigma-Aldrich), ammonium dihydrogen phosphate (NH4H2PO4) (Sigma-Aldrich), and urea ((NH2)2CO) (Alfa Aesar). All chemicals and reagents were of analytical grade. All experiments were performed using lab-produced, ultra-distilled water.
5
Hydrogel Biomaterials for Bioactive Compound Delivery
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2-Hydroxyethyl methacrylate (H, 99%) monomer and polymers of natural origin—gelatin from porcine skin (G, gel strength 300, Type A) and alginate (A)—were supplied from Sigma-Aldrich, Burghausen, Germany. Cross-linking agents N-hydroxysuccinimide (NHS) and N-ethyl-N′-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC) and polymerization agensts potassium persulfate (PPS) and N,N,N′,N′-tetramethylene diamine (TEMED) were purchased from Sigma-Aldrich, St. Louis, MO, USA. RPMI-1640 medium and supplements for cell proliferation as well as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay components were purchased from Sigma-Aldrich, St. Louis, MO, USA. All polymerizations and buffer preparations were performed in deionized water. Resveratrol (RSV) as a bioactive agent was purchased from Sigma-Aldrich, Germany.
6
Synthesis of Aniline-based Nanofibers and Nanoparticles
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Anilinium hydrochloride (ANI·HCl), aniline (ANI), and pyrrole (Py) monomers used to produce nanofibers and nanoparticles were obtained from Merck (ANI·HCl) and S. Aldrich, respectively. The chosen nanoparticles stabilizer was polyvinylpyrrolidone (PVP, Fluka, type K90, Mw = 360 000). For composites generation, N-isopropylacrylamide (NIPAM) and 2-acrylamide-2-methylpropano sulfonic acid (AMPS) monomers were purchased from Scientific Polymer Products. N,N-Methylenebisacrylamide (BIS) from Sigma-Aldrich was used as crosslinker agent. Initiator system of polymerization: ammonium persulfate (APS) and N,N,N′,N′-tetramethylenediamine (TEMED), obtained from Sigma-Aldrich. Water was triply distilled. All other reagents and solvents in this work were used as received and are analytical quality.
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