Lambda 35 spectrometer

All manipulations were carried out
at room temperature under argon using standard Schlenk, high vacuum,
and glovebox techniques using dry and degassed solvents. C₆D₆, C₆D₅CD₃, and THF-d₈ were vacuum transferred from potassium. NMR
spectra were recorded at 298 K (unless otherwise stated) on Bruker
Avance 400 and 500 MHz NMR spectrometers and referenced as follows:
C₆D₆ (¹H, δ 7.16; ¹³C, δ 128.0), C₆D₅CD₃ (¹H, δ 2.09), THF-d₈ (¹H, δ 3.58; ¹³C, δ 25.3). ³¹P{¹H} spectra were referenced externally to 85% H₃PO₄ and ¹¹⁹Sn to SnMe₄. IR
spectra were recorded on a Nicolet Nexus spectrometer and UV–vis
spectra on a PerkinElmer Lambda 35 spectrometer. Elemental analyses
were performed by Elemental Microanalysis Ltd., Okehampton, Devon,
U.K. [Ru(PPh₃)₃HCl]·toluene,^{43 (link)} [Ru(PPh₃)(C₆H₄PPh₂)₂H][Li(THF)₂] (1)⁴ and IMe₄,^{44 (link)} were
prepared according to literature methods. Prior to use, [Ru(PPh₃)₃HCl]·toluene was dried under high vacuum
and ground to a fine powder affording a material with ca. 1 molecule
of toluene per Ru (based on ¹H NMR analysis). IMe₄ was purified by sublimation. LiCH₂TMS was used as a colorless
solid obtained upon cooling a commercial 1.0 M solution in pentane
at −32 °C, separating the resulting colorless crystals
by decantation in a glovebox and drying under vacuum. AlMe₂Cl (1.0 M solution in hexane, Merck) and SnMe₃Cl (Merck)
were used as received.

UV–vis absorption spectra of NBDH, NBD-PD, SBA-NBDH, and SBA-NBD-PD, in solid state/powder, were performed with a Perkin Elmer Lambda 35 spectrometer equipped with an integrating sphere, using a spectral on as a certified reflectance standard, at a scanning speed of 60 nm/min and a slit of 4 nm. Fluorescence emission and excitation spectra of NBDH, NBD-PD, SBA-NBDH, and SBA-NBD-PD, in solid state/powder, were registered using the Jasco FP-6500 spectrofluorometer, equipped with EFA-383 epifluorescence attachment, at a scanning speed of 100 nm/min and an excitation wavelength of 450 nm.

The UV/Vis absorption spectra and fluorescence spectra were scanned on a Perkin Elmer LAMBDA 35 spectrometer (PerkinElmer, Waltham, MA, USA) and a Hitachi F-7000 (Hitachi, Tokyo, Japan) respectively. ¹H NMR spectra were obtained on a Bruker (400 MHz) spectrometer (Bruker, Karlsruhe, Germany). The excitation and emission slits were 5 nm and the PTM voltage of the spectrometer were 400 V.
The absorption and fluorescence spectra of probe A and probe B were measured in methanol (MeOH), EtOH and DCM, dimethyl formamide (DMF), and dimethyl sulfoxide (DMSO) respectively.