For light and transmission electron microscopy (TEM), the samples were washed in PBS buffer after fixing overnight at 4 °C. Then samples were post-fixed with 1% OsO4 in PBS for 2 h at 4 °C following stepwise ethanol and acetone dehydration and infiltration with Spurr’s epoxy resin. The treated samples were embedded and polymerized in Spurr’s epoxy resin at 60 °C for 48 h. Sections for light microscopy were cut using a LEICA EM UC 7 instrument with a glass knife and stained with 1% toluidine blue. The obtained specimens were photographed with an OLYMPUS BX53 camera. Ultra-thin sections (70 nm) for TEM were also cut using a LEICA EM UC 7 instrument and double-stained with 2% uranyl acetate and Sato’s lead citrate. The obtained specimens were examined with a transmission electron microscope (Hitachi-7700) at 120 kV.
Em uc7
The Leica EM UC7 is an ultramicrotome designed for cutting ultrathin sections of samples for transmission electron microscopy (TEM) analysis. It features a precision-engineered cutting mechanism that allows for the preparation of high-quality ultrathin sections with thicknesses ranging from 15 to 500 nanometers.
Lab products found in correlation
753 protocols using em uc7
Ultrastructural Analysis of Plant Fronds
For light and transmission electron microscopy (TEM), the samples were washed in PBS buffer after fixing overnight at 4 °C. Then samples were post-fixed with 1% OsO4 in PBS for 2 h at 4 °C following stepwise ethanol and acetone dehydration and infiltration with Spurr’s epoxy resin. The treated samples were embedded and polymerized in Spurr’s epoxy resin at 60 °C for 48 h. Sections for light microscopy were cut using a LEICA EM UC 7 instrument with a glass knife and stained with 1% toluidine blue. The obtained specimens were photographed with an OLYMPUS BX53 camera. Ultra-thin sections (70 nm) for TEM were also cut using a LEICA EM UC 7 instrument and double-stained with 2% uranyl acetate and Sato’s lead citrate. The obtained specimens were examined with a transmission electron microscope (Hitachi-7700) at 120 kV.
Electron Microscopy Sample Preparation
Immunolocalization of hpaXm Protein in Transgenic Plants
Colloidal-gold was preserved in our laboratory. Leaf samples of the tested transgenic lines expressing the full-length and the N-terminal leader peptide deleted mutant of hpaXm were fixed, washed, dehydrated, penetrated, embedded, and polymerized, with the tested vector-expressing transgenic line as control [20 , 44 ]. Then the leaf samples were cut into about 6-nm-thick slices and adsorbed on copper web under the ultramicrotome of Leica-EM UC7 [18 (link), 44 ]. Copper webs containing the leaf samples were moist, closed, and incubated with the diluted hpaXm-pAb twice [18 (link), 44 ]. After being washed and dyed, copper webs were observed under a Hitachi H-7650 electron microscope [18 (link), 44 ]. All tested samples of each group were repeated at least twice.
Ultrastructural Analysis of Cells by Transmission Electron Microscopy
Ultrastructural Analysis of Mitochrondria in Substantia Nigra
Ultrastructural Analysis of GABA Receptors
After several washes in TBS, hippocampal sections were incubated in the primary antibody (anti-GABAB1 at a concentration of 3–5 µg/mL and diluted in TBS containing 1% NGS). After several washes in TBS, the sections were incubated in goat anti-rabbit IgG coupled to 1.4 nm gold (Nanoprobes Inc., Stony Brook, NY, USA). Next, hippocampal sections were postfixed in 1% glutaraldehyde, washed in double-distilled water and silver enhancement of the gold particles (HQ Silver kit, Nanoprobes Inc.). Following treatment with osmium tetraoxide (1% in 0.1 M phosphate buffer), block-staining with uranyl acetate and dehydration in graded series of ethanol, the sections were then flat-embedded on glass slides in Durcupan (Fluka) resin. After polymerization of the resin, regions of interest were cut at 70–90 nm on an ultramicrotome (Leica EM UC7, Leica, Wetzlar, Germany) and collected on pioloform-coated copper grids. Finally, ultrathin sections were stained on drops of 1% aqueous uranyl acetate and Reynolds’s lead citrate. For ultrastructural analyses we used a JEOL-1010 electron microscope.
Ultrastructural Analysis of Induced Cardiomyocytes
Quantifying Mitochondrial Damage in Ischemic Cortex
Electron Microscopy Sample Preparation
Transmission Electron Microscopy Protocol
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