Plasma samples were obtained from 30-week-old control rats and from 27–32 week-old DM-cont and DM-empa rats after the 12-week treatment period. The plasma samples were treated with Phenomenex EZ:faast AA analysis kit (Phenomenex, Torrance, CA), with norvalin used as the internal standards [18 (link)]. GCMS-QP2010Plus and GCMS solution software (Shimadzu, Kyoto, Japan) were used for GC-MS analysis [19 (link)].
Ez faast aa analysis kit
Manufactured by Phenomenex
The Phenomenex EZ:faast AA analysis kit is a comprehensive solution for the rapid and accurate analysis of amino acids in various sample types. The kit includes all the necessary components for sample preparation, derivatization, and analysis using gas chromatography-mass spectrometry (GC-MS) or high-performance liquid chromatography (HPLC) techniques. The kit provides a streamlined workflow and reliable results, making it a valuable tool for laboratories focused on amino acid quantification.
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2 protocols using ez faast aa analysis kit
1
GC-MS Analysis of Plasma Amino Acids
2
Amino Acid and Metabolite Profiling in Serum
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For analysis of AA concentrations, serum samples were processed using the Phenomenex EZ:faast AA analysis kit (Phenomenex) for GC-MS with norvalin and an isotopically labeled AA mixture (Cambridge Isotope Laboratories) as internal standards (38 (link), 39 (link)). AAs were analyzed on a Hewlett Packard 6890 gas chromatograph interfaced to a Hewlett Packard 5975 mass selective detector (Agilent Technologies). For the analysis of arginine, serum samples were treated with arginase (Creative Enzymes) for 20 min at 37°C to convert arginine to ornithine. Ornithine was then quantified after sample processing as described above. Arginine concentration was calculated as the difference of ornithine concentrations before and after arginase treatment. The CVs for individual AAs ranged from 1.4% to 5.1%.
Total LDL cholesterol, HDL cholesterol, TGs, and FFAs as well as transaminases were measured on a Cobas c311 analyzer (Roche Diagnostics) (25 (link)). Plasma fibroblast growth factor 21 (FGF21) concentrations were measured with the Human FGF21 Quantikine ELISA [R&D Systems (Bio-techne)] as described previously (40 (link)).
Total LDL cholesterol, HDL cholesterol, TGs, and FFAs as well as transaminases were measured on a Cobas c311 analyzer (Roche Diagnostics) (25 (link)). Plasma fibroblast growth factor 21 (FGF21) concentrations were measured with the Human FGF21 Quantikine ELISA [R&D Systems (Bio-techne)] as described previously (40 (link)).
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