HPLC-PDA-MSn mass spectra was performed through a ThermoFinnigan (Thermo Electron Corporation, Austin, TX, USA) LC system coupled with a mass spectrometer (LCQ-Duo ion trap) having an ESI source (ThermoQuest, Thermo Scientific, Waltham, MA, USA) [15 (link)]. The injection process, flow rate, elution solvents, resolution, and negative MS operating parameters were described previously [17 (link)]. In brief, a Zorbax Eclipse XDB-C18, rapid resolution, 150 × 4.6 mm, 3.5 μm column was used (Agilent, Santa Clara, CA, USA). A gradient consisting of water and acetonitrile (ACN), each having 0.1% formic acid, was applied, and ACN was increased from 5% to 30% within 60 min and then to 90% within the next 30 min at a flow rate of 1 mL/min and a 1:1 split before the ESI source [17 (link)].
Thermoquest
Manufactured by Thermo Fisher Scientific
Sourced in United States
ThermoQuest is a precision laboratory instrument designed for analytical applications. It provides accurate and reliable performance for various scientific measurements and analyses. The core function of ThermoQuest is to enable precise data collection and analysis within laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Thermo finnigan, by Thermo Fisher Scientific (2 mentions)
Lcq duo ion trap, by Thermo Fisher Scientific (1 mentions)
Db 1701, by Agilent Technologies (1 mentions)
Lcq duo ion trap mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Xcalibur 2, by Thermo Fisher Scientific (1 mentions)
Zorbax eclipse xdb c18, by Agilent Technologies (1 mentions)
Chrompack cp sil88, by Agilent Technologies (1 mentions)
4 protocols using thermoquest
1
HPLC-PDA-MSn Analysis of Compounds
2
Volatile Organic Compounds in Oysters
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The VOCs were analyzed for five individual oyster (80-120 g) per experimental group. For every individual oyster 10 ml of NaCl saturated ultrapure water was added to 5 g oyster tissue prior to homogenization. Homogenization was performed for one minute using a T25 Ultra Turrax homogenizer (IKA Werke GmbH, Germany) ensuring that the sample remained in ice preventing excess heat. VOCs were extracted by HeadSpace Solid Phase Micro Extraction (HS-SPME) and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) according to Fratini, Lois, Pazos, Parisi and Medina (2012) . GC-MS analysis was performed in a Thermo Finnigan ThermoQuest (USA) gas chromatograph equipped with a split/splitless injector and coupled to a trace quadrupole mass detector (Thermo Finnigan ThermoQuest, USA). Compounds were separated in a capillary column (30 m × 0.250 mm × 1 μm film thickness, fused silica DB-1701, Agilent Technologies, USA). All analyses were performed setting ionization energy at 70 eV, filament emission current at 150 μA and the electron multiplier voltage at 500 V (Fratini et al., 2012) and the spectra were acquired in full scan mode.
3
LC-MS Analysis of Bioactive Compounds
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The LC system was Thermo Finnigan (Thermo Electron Corporation, Waltham, MA, USA). The reversed-phase column (Zorbax Eclipse XDB-C18, rapid resolution, 4.6 × 150 mm, 3.5 µm, Agilent, Santa Clara, CA, USA) was used. The mobile phase was water and acetonitrile (ACN) (0.1% formic acid each) and gradient was employed from 5 % to 30% CAN in 60 min with flow rate 1 mL/min with a 1:1 split before the ESI source. Autosampler surveyor ThermoQuest (Thermo Electron Corporation, Waltham, MA, USA) was used to inject the sample; the process was controlled by Xcalibur software (XcaliburTM 2.0.7, Thermo Scientific, Waltham, MA, USA) [23 (link)]. LCQ-Duo ion trap mass spectrometer (ThermoQuest Corporation, Austin, TX, USA) with an ESI source (ThermoQuest) was used and operated in the negative mode as described before [22 (link)]. Full scan mode with a mass range of 50–2000 m/z was employed to detect the ions.
4
Gas Chromatography Fatty Acid Analysis
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Fatty Acids. Details about the FA analyses were reported in Pegolo et al. (2016) . Briefly, FAME were prepared by the direct extraction and alkali-catalyzed trans-methylation procedure, previously described by Feng et al. (2004) . The FA composition was determined using a ThermoQuest gas chromatograph (Thermo Electron Corp., Waltham, MA) equipped with a flameionization detector and a high polar fused-silica capillary column (Chrompack CP-Sil88 Varian, Middelburg, the Netherlands; 100 m, 0.25 mm i.d.; film thickness 0.20 µm). The operating conditions of the GC apparatus were as follows: helium carrier gas at a flow rate of 1 mL/min; split ratio: 1:80; oven temperature: 60°C held for 1 min, increased to 173°C at 2°C/min and held for 30 min, increased to 185°C at 1°C/min and held for 5 min, and finally increased to 220°C at 3°C/min and held for 19 min; injector temperature: 270°C; detector temperature: 300°C.
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