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Neurotrack software module

Manufactured by Sartorius
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NeuroTrack is a software module that provides automated analysis of neurological data. It is designed to process and analyze information collected from various neurological research instruments. The core function of NeuroTrack is to enable researchers to efficiently extract and interpret insights from their neurological data.

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Lab products found in correlation

Incucyte zoom imaging system, by Sartorius (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

IncuCyte NeuroTrack Software Module (5 citations) Incucyte Neurotrack Analysis Software Module (2 citations)

3 protocols using neurotrack software module

1

Quantifying Neurite Outgrowth after Gene Knockdown

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96 hours after transfection, cells were imaged on an IncuCyte ZOOM Imaging System (Essen Bioscience) at 10X or 20X magnification. Neurite outgrowth was quantified and a neurite definition for each cell line was created using the NeuroTrack software module (Essen BioScience). Knockdown efficiency for each siRNA was validated at 48 hours by qRT-PCR and decreases in mRNA expression of 85% or more were observed (qPCR method described below).
Kosti A., Du L., Shivram H., Qiao M., Burns S., Garcia J.G., Pertsemlidis A., Iyer V.R., Kokovay E, & Penalva L.O. (2019). ELF4 is a target of miR-124 and promotes neuroblastoma proliferation and undifferentiated state. Molecular cancer research : MCR, 18(1), 68-78.
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2

Quantifying Neurite Outgrowth in BE-(2)-C Cells

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Ninety-six hours after transfection, cells were imaged by an IncuCyte at 10× or 20× magnification. Neurite outgrowth was quantified on an IncuCyte ZOOM Imaging System (Essen BioScience). A neurite definition specific to BE-(2)-C cells was created using the NeuroTrack software module (Essen BioScience). Experiments were performed using biological duplicates and technical quadruplicates.
Velasco M.X., Kosti A., Guardia G.D., Santos M.C., Tegge A., Qiao M., Correa B.R., Hernández G., Kokovay E., Galante P.A, & Penalva L.O. (2019). Antagonism between the RNA-binding protein Musashi1 and miR-137 and its potential impact on neurogenesis and glioblastoma development. RNA, 25(7), 768-782.
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3

Neuroblastoma Differentiation Assay

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Neuroblastoma BE-(2)-C cells were obtained from the ATCC and grown in DMEM/F12 (GIBCO) supplemented with 10% fetal bovine serum (GIBCO) and 1% Pen/Strep (GIBCO). BE-(2)-C cells were infected with pUltra-Control or pUltra-SERBP1 virus. Seventy-two hours later, infected cells were plated into 96-well plate at 2000 cells per well and treated with or without 10 μM retinoic acid. Neurite outgrowth was quantified on an IncuCyte ZOOM Imaging System (Essen BioScience) 4 days after platting. For each cell line, a neurite definition was created using the NeuroTrack software module (Essen BioScience). All experiments were performed with biological and technical triplicates.
Kosti A., de Araujo P.R., Li W.Q., Guardia G.D., Chiou J., Yi C., Ray D., Meliso F., Li Y.M., Delambre T., Qiao M., Burns S.S., Lorbeer F.K., Georgi F., Flosbach M., Klinnert S., Jenseit A., Lei X., Sandoval C.R., Ha K., Zheng H., Pandey R., Gruslova A., Gupta Y.K., Brenner A., Kokovay E., Hughes T.R., Morris Q.D., Galante P.A., Tiziani S, & Penalva L.O. (2020). The RNA-binding protein SERBP1 functions as a novel oncogenic factor in glioblastoma by bridging cancer metabolism and epigenetic regulation. Genome Biology, 21, 195.
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