Vertex 70 ftir spectrometer

The inclusions for n-hexane, ethyl acetate and dicholoromethane (DCM) extracts were prepared in a 1:1 ratio. Fifty mg of extracts were dissolved in 50 mL of methanol and these extracts were carefully filtered. Then, almost 50 mg β-CD (in 50 mL of water) was added and the mixture was stirred at room temperature for 24 h. After that, the mixtures were filtered, and the methanol was removed using a rotary evaporator. The final mixtures were lyophilized at −40 °C to remove all water and the dry samples were stored in the dark condition at 4 °C. The obtained inclusions were named hexane/β-CD, ethyl acetate/β-CD and DCM/β-CD.
The samples were directly placed on aluminum stabs; then, the shape and surface characteristics were observed by micrographs of the samples taken by scanning electron microscopy (SEM) (Zeiss EVO LS10; Oberkochen, Germany) using the gold sputter technique. The zone magnification for the images were kept around 20,000×. Observations were performed under 1 and 15 kV. Fourier transform infrared spectrum (FTIR) was recorded on a Vertex 70 Bruker FTIR spectrometer (Bruker, Leipzig, Germany) with attenuated total reflectance (ATR) module. All spectra were captured in the 400 and 4000 cm⁻¹ spectral region at a scan rate of 180 scans and a spectral resolution of 4 cm⁻¹. The FTIR spectrum was used in the transmittance mode.

FTIR analysis was accomplished on a Vertex 70 Bruker FTIR spectrometer (Billerica, MA, USA) using an attenuated total reflectance (ATR) addition. For all the obtained biomaterials, the FTIR spectra were recorded in the ATR-FTIR method (in triplicate) at a resolution of 4 cm⁻¹ in the 600–4000 cm⁻¹ wavenumber range.

The structural characterization of raw material and GelMA with different methacrylation degrees was performed using ATR-FTIR spectrometry. FTIR spectra were recorded on a Vertex 70 Bruker FTIR spectrometer (Bruker, Billerica, MA, USA) equipped with an attenuated total reflectance (ATR) accessory. For all the formulations, the FTIR spectra were registered in the ATR mode, at a resolution of 4 cm⁻¹ in the 600–4000 cm⁻¹ wavenumber region.

The samples were structurally evaluated using Vertex 70 Bruker FTIR spectrometer (Billerica, MA, USA). All the FT-IR analyses were achieved in the 4000–600 cm⁻¹ wavelength scale. The grounded samples were used for qualitative FTIR analysis in ATR mode.

For transmission FTIR spectra acquisition, the tip of one SRT was ground and the powder was mixed with dry pulverized KBr (Merck KGaA) and pressed into a pellet. The infrared spectra were acquired using a Vertex 70 FT-IR-Spectrometer (Bruker GmbH, Germany) with a Deuterated Triglycine Sulfate (DTGS) detector. A total of 32 scans were co-added per sample spectrum (wavenumber range: 600–4000, cm⁻¹), background subtracted (concave rubberband correction, 2 iterations) and smoothed (9 smoothing points). For data processing and plotting, Opus 7.0 (Bruker) and OriginPro 8.6 were used.