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Rabbit anti bid

Manufactured by Proteintech
Sourced in United States

Rabbit anti-Bid is a primary antibody that specifically binds to the Bid protein. Bid is a pro-apoptotic member of the Bcl-2 family of proteins, which play a key role in the regulation of apoptosis (programmed cell death). This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect and analyze the Bid protein.

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2 protocols using rabbit anti bid

1

Western Blot Analysis of Apoptosis Markers

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Mouse anti-Bax, mouse anti-Bcl-2, rabbit anti-Caspase-3, rabbit anti-Bid, rabbit anti-PARP, rabbit anti-Survivin, and rabbit anti-GAPDH were obtained from Proteintech (USA). Horseradish peroxidase- (HRP-) conjugated goat anti-mouse and anti-rabbit IgG antibodies were provided by Beijing Zhongshan Golden Bridge Biotechnology Co. Ltd. (Beijing, China). Protein assay kit was obtained from Beijing Pulilai Gene Technology Co., Ltd. (Beijing, China). Reverse transcription kit was obtained from Promega (USA).
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2

Synthesis and Characterization of HA-DPA-β-lap Conjugate

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N-hydroxy succinimide (NHS) and 3,3′-dithiodipropionic acid (DPA) were purchased from Sigma-Aldrich (Beijing, China). N,N-Dicyclohexylcarbodiimide (DCC) were purchased from Alfa Aesar (Shanghai, China). Hyaluronic acid (HA) was purchased from Macklin (Shanghai, China). N,N-Dimethylformamide (DMF) and dimethyl sulfoxide (DMSO) were purchased from Aladdin (Shanghai, China), β-lapachone (abbreviated as β-lap) was provided from yuanye Bio-Technology (Shanghai, China). Primary antibodies including rabbit anti-BID, rabbit anti-AIF, rabbit anti-EndoG, mouse anti-GAPDH and mouse anti-GPX4 were brought from Proteintech (Wuhan, China). Cell counting kit-8 (CCK-8) was purchased from Sevenbio (Beijing, China). Hoechst 33342 staining solution and mitochondrial membrane potential assay kit with JC-1 were purchased from Beyotime Biotechnology (Shanghai, China), reactive oxygen species assay kit and Fluo-4 AM probe were brought from Solarbio Science & Technology Co., Ltd. (Beijing, China).
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