Agilent 7890 gas chromatograph

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 7890 gas chromatograph is a laboratory instrument used for the separation, identification, and quantification of volatile and semi-volatile organic compounds. It features a temperature-controlled oven, a sample injection system, and multiple detector options to suit various analytical needs.

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50 protocols using agilent 7890 gas chromatograph

GC-MS Analysis of Volatile Compounds

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The GC-MS analyses followed our previous work [37 (link)]. In brief, tetradecane was dissolved in methanol and the solution was used as internal standard. Before extraction, 10 μL of tetradecane (5.0 mg/L) were placed into the bottom of the vial. The sample vial caps were replaced by crimp-top silicon rubber caps with a Teflon layer and maintained at 60 °C in a water bath. Subsequently, the volatile compounds were extracted by SPME with a 2 cm, 50/30 µm, coated DVB/CAR/PDMS fiber supplied by Supelco (Bellefonte, PA, USA) and the extraction time was 60 min.
The determination was conducted using an Agilent 7890 gas chromatograph (Agilent, Santa Clara, CA, USA) fitted with an Agilent 5975C mass spectrometer (Agilent). Volatile compounds were injected in the splitless mode injector (splitless time of 0.75 min) heated at 240 °C for 7 min and separated on a DB-5 capillary column (30 m × 0.25 mm × 0.25 μm; Agilent). Helium was used as carrier gas with a constant flow rate at 1.0 mL/min. The temperature program was as follows: 35 °C for 1 min, and then increased to 230 °C at 5 °C /min, and finally increased to 280 °C at 20 °C /min. Electron ionization (EI-MS) mode was carried out at 70 eV and a mass scan range from m/z 35 to 330 atomic mass units (amu).

Cheng Z., Li M., Marriott P.J., Zhang X., Wang S., Li J, & Ma L. (2018). Chemometric Analysis of the Volatile Compounds Generated by Aspergillus carbonarius Strains Isolated from Grapes and Dried Vine Fruits. Toxins, 10(2), 71.

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Cuticular Hydrocarbon Extraction and Analysis

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The cuticular hydrocarbons were extracted from four haphazardly chosen nymphs clutch in 100 μL heptane (C 7 H 16 , Merck, Zug, Switzerland) containing 2.5 ng/μL of internal standard (n-octadecane, C 18 H 38 , Sigma-Aldrich, Buchs, Switzerland) for the duration of 10 min. Subsequently, 60 μL of the extract were pipetted into an additional conical bottom autosampler vial. Chemical analysis was conducted with an Agilent7890 gas chromatograph (GC) coupled to an Agilent5975C inert XC MSD mass spectrometer (MS). Two μl of each sample were injected into the GC-MS using a splitless mode and a DB5 column (HP-5 ms: length: 30 m, inner diameter: 0.25 mm, film thickness: 0.25 μm, Agilent Technologies, Basel, Switzerland). The SSL injector temperature was held constant at 250 °C and a helium flow rate of 1 mL/s over the whole duration of the GC run. GC oven temperature started at 70 °C, was initially held for 2 min, then ramped at 15 °C/ min to 232 °C (where it was held for 11 min), at 5 °C/min to 263 °C and 15 °C/min to 300 °C (where it was held for 7 min).
The chemical compounds were identified by comparing the mass spectra of the peaks with the mass spectral library NIST2008 and with former identifications of F. auricularia CHC profiles (Wong et al. 2014a, b) .

Boos S., Röllin L., Wong J.W.Y, & Kölliker M. (2015). Maternal Transfer of Cuticular Hydrocarbons? Evidence from Between-Species Cross-Fostering Experiments in Earwigs. J Insect Behav., 28(3).

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Metabolite Profiling of Plant Root Exudates

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The root exudates were analyzed by a GC–TOF–MS system at Shanghai Biotree Biotechnology Limited Company, Shanghai, China. The GC–TOF–MS system consisted of an Agilent 7890 gas chromatograph coupled to a time-of-flight mass spectrometer. A DB-5MS capillary column (30 m × 250 μm × 0.25 μm, J&W Scientific, Folsom, CA, USA) was used for this system. The methods of extraction of root exudates and GC–TOF–MS analysis have been described in detail in a previous study [41 (link)]. The peaks in QC samples with detection rate <50% or relative standard deviation >30% were removed [60 (link)]. The data analysis was performed using the R (v3.6.1) program and MetaboAnalyst (v5.0). The carbon and nitrogen contents in root exudates were measured with an elemental analyzer (vario PYRO cube, Elementar). The combustion tube temperature of the instrument was 850°C and the reduction tube temperature was 650°C.

Chen L., Bian L., Ma Q., Li Y., Wang X, & Liu Y. (2024). Defensive alteration of root exudate composition by grafting Prunus sp. onto resistant rootstock contributes to reducing crown gall disease. Horticulture Research, 11(4), uhae049.

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Chromatographic Quantification of Pesticides

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The identification and quantification of the analytes was carried out through liquid chromatography (LC) using a Waters (Milford, MA, USA) module equipped with a model e2695 multisolvent delivery and autosampler system coupled to a Micromass-ZQ single quadrupole mass spectrometer detector with an ESI interface, and gas chromatography using an Agilent 7890 gas chromatograph coupled to an Agilent 5975 MSD mass spectrometer (Agilent Technologies, Wilmington, DE, USA), under conditions proposed byHerrero-Hernandez et al. (2013 ( ) andCliment et al. (2018)) ,respectively. Because methamidophos, diazoxon, azinphos-methyl and chlorpyrifos oxon are included in this study, it was necessary to adjust the temperature ramps in GC-MS and the elution gradient in LC-MS in order to obtain good compound separation. Table S1 andTable S2 indicate the optimized chromatographic conditions for the analytic determination of pesticides and degradation products through LC-MS (in dissolved and particulate phases) and GC-MS (in dissolved phase).

Climent M.J., Herrero-Hernández E., Sánchez-Martín M.J., Rodríguez-Cruz M.S., Pedreros P, & Urrutia R. (2019). Residues of pesticides and some metabolites in dissolved and particulate phase in surface stream water of Cachapoal River basin, central Chile. Environmental pollution (Barking, Essex : 1987), 251.

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GC-MS Analysis of Milk Volatiles

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Volatile compounds for each milk sample were evaluated by GC-MS/solid-phase microextraction (SPME) analysis as previously described (Lanciotti et al., 2006) . A divinylbenzenecarboxen-polydimethylsiloxane-coated fibre (65 µm) and a manual SPME holder (Supelco) were used for the SPME of volatile compounds in milk. For peak detection, an Agilent 7890 gas chromatograph (Agilent Technologies, Santa Clara, FL, USA) coupled to an Agilent 5970 mass selective detector was used. This system was operated in electron impact mode with an ionisation voltage of 70 eV. The column used was a Chrompack CP-Wax 52 CB capillary column (50 m × 0.32 mm i.d.; Chrompack, Middetburg, Netherlands). The temperature was adjusted to 50 °C for 2 min and then raised 1 °C every minute up to 65 °C and after that 5 °C per min to 220 °C. The temperatures of the injector, interface, and ion source were 250, 250, and 230°C, respectively.
Injections were carried out in splitless mode, and the carrier gas was helium with a constant flow rate of 1 mL min -1 . Volatile compounds were identified using mass spectra databases (NIST/EPA/NIH version 2005). The quantification of the main volatile compounds was performed
7 on the basis of calibration curves obtained by adding pure standards to 5 mL of milk samples and prepared as previously described for volatile compound analysis.

Korachi M., Ozen F., Aslan N., Vannini L., Guerzoni M.E., Gottardi D, & Ekinci F.Y. (2015). Biochemical changes to milk following treatment by a novel, cold atmospheric plasma system. International Dairy Journal., 42.

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Gas Sampling and Chromatographic Analysis

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In total, 90 gas samples were taken (each diversity level in both temperature regimes after 1, 4 and 8 weeks of incubation in five replicates) with a 10 ml gas tight needle (Hamilton, Reno, NV; United States) and transferred into 3 ml gastight vails (Exetainer^®, Labco, United Kingdom) and stored at room temperature. To detect concentration changes in carbon dioxide, methane and oxygen, gases were quantified using an Agilent 7,890 gas chromatograph (Agilent Technologies Inc., Santa Clara, CA, United States) equipped with a ShinCarbon ST 80/100 column (2 m, 0.5 mm ID, Restek Corporation, Bellafonte, PA, United States) and a pulsed-discharge helium ionization detector. Oxygen and oxygen-dinitrogen ratios were used as indicator for aerated incubations to monitor aerobic wood decay.

Muszynski S., Maurer F., Henjes S., Horn M.A, & Noll M. (2021). Fungal and Bacterial Diversity Patterns of Two Diversity Levels Retrieved From a Late Decaying Fagus sylvatica Under Two Temperature Regimes. Frontiers in Microbiology, 11, 548793.

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Electrophysiological Responses of Armyworm Moth

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The antennal electrophysiological responses of male and female A. segetum to the synthetic mixture of C₆-C₁₀ straight chain acids were recorded on an Agilent 7890 gas chromatograph equipped with a flame ionization detector (FID) (Agilent Technologies, USA) and an electroantennographic detector (EAD) (Syntech, Germany). An Agilent J&W HP-5 column (30 m × 0.25 mm i.d., 0.25 μm film thickness; Agilent Technologies, USA) was used in the GC, where the inlet temperature was set at 250 °C, the transfer line was heated at 255 °C, and the detector was set at 280 °C. Hydrogen was used as the carrier gas at a constant flow of 1.8 mL/min, and a 1:1 division of the GC effluent was directed to the FID and EAD, respectively. A PRG-2 EAG (10x gain) probe (Syntech, Germany) was used in the recording. After cutting off the tips, both antennae associated with the head of a 1–2-day-old adult were mounted on the probe using conductive gel (Blågel, CefarCompex, Sweden). Charcoal-filtered and humidified air passed over the antennal preparation. The GC oven was programmed from initial hold at 100 °C for 2 min, then increased to 160 °C at a rate of 10 °C/min and then held for 2 min at the final temperature. For each recording, a synthetic mixture containing 500 ng of each acid was injected in split mode (ratio of 40:1). Data were collected with the software GC-EAD Pro Version 4.1 (Syntech, Germany).

Hou X.Q., Zhang D.D., Powell D., Wang H.L., Andersson M.N, & Löfstedt C. (2022). Ionotropic receptors in the turnip moth Agrotis segetum respond to repellent medium-chain fatty acids. BMC Biology, 20, 34.

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GC-TOF-MS Analysis of Metabolite Profiles

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An Agilent 7890 gas chromatograph (Agilent, USA) was used in conjunction with a Pegasus^® HT time-of-flight mass spectrometer (LEGO, USA) for gas chromatography time-of-flight mass spectrometry (GC/TOFMS) analyses. Our setup used a 30 m × 250 μm inner diameter DB-5MS column with a film thickness of 0.25 μm (J&W Scientific, USA). We injected 1 μl of the sample for analysis. The carrier gas was helium with a purge flow of 3 ml/min and a gas flow of 1 ml/min. The temperature cycle was as follows: 50 °C for 1 min, which was then increased to 300 °C at a rate of 10°C/min, and finally 300°C for 8 min. The temperatures of the injection, transfer line, and ion source were maintained at 280, 270, and 220°C, respectively and the energy was set at -70 eV. After a solvent delay of 460 s, we acquired the data with an m/z range of 50–500 at a rate of 20 spectra/s.

Kang W.S., Chen L.J., Wang Y.Y., Zhu X.F., Liu X.Y., Fan H.Y, & Duan Y.X. (2020). Bacillus simplex treatment promotes soybean defence against soybean cyst nematodes: A metabolomics study using GC-MS. PLoS ONE, 15(8), e0237194.

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Truffle Aroma Profiling using SPME

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5.0 g fresh truffle purees were accurately weighed in 20 mL vials, Teflon covers and added 5 μL internal standard solutions (100 mg/L 1, 2-dichlorobenzene or 100 mg/L 2-methyl-3 -tetrahydrofuran thiol for sulfide). Samples were kept at 45 °C in a water bath with 10 min of equilibration time.
A 50/30 μm divinylbenzene-Carboxen-polydimethylsiloxane (DVB-CAR-PDMS) fiber (Supelco, Bellefonte, PA, USA) with a 1 cm length was used. The extraction time was 45 min. Before chemical absorption, the fiber was preconditioned for 30 min on an Agilent 7890 gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) with the injector temperature of 250 °C.

Feng T., Shui M., Song S., Zhuang H., Sun M, & Yao L. (2019). Characterization of the Key Aroma Compounds in Three Truffle Varieties from China by Flavoromics Approach. Molecules, 24(18), 3305.

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Metabolomic Profiling of Rice Using GC-MS

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Metabolomic analysis using gas chromatography coupled with mass spectrometry (GC-MS) was performed according to a previous report, with slight modifications [24 (link)]. First, 100 mg rice powder was added into 1.0 mL water–methanol–dichloromethane solution (1:3:1) and 40 μL internal standard solution (200 μg/mL, adonitol). The extraction was carried out by sonication for 30 min, and the mixture was then centrifuged for 10 min at 16,000× g. Next, 0.7 mL of the upper layer solution was transferred to a 1.5 mL Eppendorf tube and vacuum-dried in a CentriVap centrifugal vacuum concentrator (Labconco, Kansas City, MO, USA). Sixty microliters of methoxyamine hydrochloride (20 mg/mL in pyridine) was added to each vial and incubated for 30 min at 80 °C. Subsequently, 80 μL BSTFA reagent (1% TMCS, v/v) was added, followed by a reaction time of 1.5 h at 70 °C. GC-MS analysis was conducted using an Agilent 7890 gas chromatograph (Agilent Technologies, Palo Alto, CA, USA) system equipped with a Shimadzu GC–MS QP2010 Plus (Shimadzu, Kyoto, Japan). The instrumental settings followed previous protocols [24 (link)].

Xu J., Zhong J., Zhang B, & Li X. (2021). Green Labelled Rice Shows a Higher Nutritional and Physiochemical Quality Than Conventional Rice in China. Foods, 10(5), 915.

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