N hexane

1,4-Cyclohexanedimethanol, 1,5-pentanediol, 2,2-dimethoxypropane, and p-toluene sulfonic acid (PTSA) were purchased from the Aladdin Reagent Company (Shanghai, China). Cyclohexane, ethyl acetate, triethylamine, and n-hexane were bought from Sinopharm Chemical Reagent Company (Shanghai, China) and distilled with calcium hydride. NaAlO₂ (57.1% Al₂O₃ and 37.2% Na₂O) and NaOH (96 wt%) were gained from Sinopharm Chemical Reagent Company (Shanghai, China) and fumed silica was obtained from the Shanghai Tengmin Industrial Company (Shanghai, China). Commercial H type ZSM-5 zeolite with a Si/Al molar ratio of 12, denoted as C-Z5, was acquired from Nankai University Catalyst Co., Ltd. (Tianjin, China). Low-density polyethylene (LDPE) powder used as a model feedstock in the cracking reaction was provided by Alfa Aesar (Stock #42607) (Tianjin, China).

Two types of fish oils, namely triglycerides fish oil (19.21% EPA and 10.81% DHA) and ethyl ester fish oil (42.47% EPA and 31.91% DHA), were kindly provided by the Fujian Coland Marine Bio‐engineering Co., Ltd (Fujian, China). Novozyme 435 (Candida antarctica lipase immobilized on acrylic resin) and lipozyme TL IM, an inexpensive 1, 3‐position‐specific lipase from Thermomyces lanuginosus, were purchased from Novozymes A/S (Bagsvaerd, Denmark). Porcine pancreas lipase (PPL) was purchased from Shanghai Sanjie Co., Ltd (Shanghai, China). Ionic liquids, namely 1‐butyl‐3‐methylimidazolium tetrafluoroborate ([BMIM][BF₄]), 1‐butyl‐3‐methylimidazolium hexafluorophosphate ([BMIM][PF₆]), and 1‐butyl‐3‐methylimidazolium bis(trifluoromethanesulfonyl)imide ([BMIM][Tf₂N]), were purchased from Shanghai Chengjie Chemical Co., Ltd (Shanghai, China). N‐octanoic acid and n‐hexane (HPLC grade) were purchased, respectively, from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) and Shandong Yuwang industrial Co., Ltd (Shandong, China). Thin‐layer chromatography silica gel G (analytical grade) was purchased from Qingdao Marine Chemical Co., Ltd (Shandong, China). Boron trifluoride diethyl ether and carboxymethyl cellulose sodium (analytical grade) were purchased from Aladdin Chemicals Co., Ltd (Shanghai, China).

The spinnable pitch was provided by Jiexiu Changlong New Material Technology Co., LTD., China. This spinnable pitch that resulted in air-blown thermal polycondensation was an isotropic pitch. The analytical reagents of n-hexane, toluene, and tetrahydrofuran were purchased from Sinopharm Group (China).

Octachlorodipropyl ether (OCDPE, 99.6%) was purchased from National Pesticide Product Quality Supervision and Inspection Center (Beijing, China). Anhydrous sodium sulfate, ethyl acetate, n-hexane, petroleum ether, and xylene (analytical grade) were all purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China). Agricultural emulsifier 2201 (Calcium dodecylbenzenesulfonate: styrene, phenol, formaldehyde condensates, polyoxyethylene ether (emulsifier 400-3#): dimethylbenzene = 30:43:26, v/v) was purchased from Haian Petrochemical Factory (Nantong, China).
Gas chromatography (GC) was performed using an Agilent 6890 Series instrument from Agilent Technologies (Santa Clara, CA, USA). An EYELAN-1100 rotary evaporator was used from the Shanghai Ailang Instruments Co., Ltd. (Shanghai, China). A TG16-WS high-speed centrifuge was used from Changsha Xiangyi Centrifuge Instrument Co., Ltd. (Changsha, China). A SB-3200 ultrasonic cleaner was used from Kunshan Ultrasonic Instrument Co., Ltd. (Shanghai, China). A DHG-9070A electrothermal oven thermostat blast was used from the Shanghai Yiheng Science and Technology Co., Ltd. (Shanghai, China).

Mature seeds from the mAppA transgenic line were pulverized to permit passage through a 0.38 mm sieve³⁶ . The extraction mixture consisted of 1 g of collected soybean flour^{42 (link)} and 5 mL of n-hexane (97%, Sinopharm Chemical Reagent Co., Ltd). The ratio of solvent to beans was 5:1^{43 (link)}. Experiments were performed at 55, 60 or 65 °C^{44 (link),45 (link)}, with extraction time limit set to 1, 2 or 3 hs at each temperature point. All the extractions were carried out in triplicate. At the end of the contact time, the supernatants were discarded by centrifugation (15938 g, 10 min) and traces of solvent remaining were removed by evaporation. The resulting precipitation was homogenized in 10 mL of 0.2 M sodium acetate buffer (pH 5.0) for phytase extraction. The phytase in the control sample (1 g of soybean flour without n-hexane extraction) was extracted simultaneously with 10 mL of 0.2 M sodium acetate buffer (pH 5.0). After centrifugation (15938 g, 10 min), the supernatants were collected and assayed for phytase activity using the standard method. The phytase activity per gram for both non n-hexane-extracted and n-hexane extracted samples were calculated based on original pulverized soybean weight.

Anhydrous ethanol, phenol, sulfuric acid, n-hexane, propanone, methylbenzene, KOH, H₂SO₄, ether, ethyl acetate, were supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Methanol, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,4,6-trihydroxybenzoic acid, were obtained from Sigma-Aldrich (St. Louis, MO, USA). A total antioxidant activity assay kit (2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS)) was garnered from Beyotime Biotechnology (Shanghai, China).