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Rabbit polyclonal anti lamin b1

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Rabbit polyclonal anti-Lamin B1 is a primary antibody raised in rabbits against Lamin B1, a type of lamin protein found in the cell nucleus. This antibody can be used for the detection and analysis of Lamin B1 in various applications.

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Lab products found in correlation

Substrate chemiluminescence kit, by Merck Group (2 mentions) Mouse monoclonal anti p53, by Santa Cruz Biotechnology (2 mentions) Chemidoc imaging system, by Bio-Rad (2 mentions) Monoclonal rabbit anti p21 waf1 cip1, by Cell Signaling Technology (2 mentions) Polyclonal rabbit anti pp53 serin15, by Cell Signaling Technology (2 mentions) Monoclonal mouse anti gapdh glyceraldehyde, by Santa Cruz Biotechnology (2 mentions) Mouse monoclonal anti v5, by Thermo Fisher Scientific (1 mentions) Rabbit polyclonal anti gfp, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti tata binding protein, by Abcam (1 mentions) Tcs sp5, by Leica (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti nxf1, by Abcam (1 mentions) Mouse monoclonal anti gapdh pod, by Merck Group (1 mentions) Mab414, by Abcam (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Cisplatin, by Selleck Chemicals (1 mentions) Aldh1, by BD (1 mentions) Doxycycline, by Selleck Chemicals (1 mentions)

Spelling variants of this product

Lamin B1 (174 citations) Anti-Lamin B1 (86 citations) Rabbit anti-Lamin B1 (31 citations) Rabbit anti- (5 citations) Rabbit Lamin B1 (3 citations)

9 protocols using rabbit polyclonal anti lamin b1

1

Western Blot Antibody Validation

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The following primary antibodies were used for WBs: mouse monoclonal anti-V5 (1:5000 dilution WB, ThermoFisher Scientific, cat. no. R960-25), rabbit polyclonal anti-GFP (1:1500 dilution WB, Cell Signaling, Danvers, MA, USA; cat. no. 2555), rabbit polyclonal anti-Lamin B1 (1:1500 dilution WB, Abcam, cat. no. ab16048), mouse monoclonal anti-β-tubulin (1:1000 dilution WB, Abcam, cat. no. ab7792), mouse monoclonal anti-TATA Binding Protein (TBP) (1:2000 dilution WB, Abcam, cat. no. ab818), goat polyclonal β-catenin C-18 (1:500 dilution WB, Santa Cruz Biotechnology, Dallas, TX, USA; cat. no. sc-1496), and mouse monoclonal anti-UBC9 (1:1000 dilution WB, BD Biosciences, Franklin Lakes, NJ, USA; cat no. 610748). Secondary antibodies used for WB (1:5000 dilution) were: horse radish peroxidase (HRP)-conjugated rabbit anti-mouse, rabbit anti-goat, and goat anti-rabbit (Zymed, ThermoFisher Scientific). All antibodies were diluted in blocking buffer (5% skim milk [Diploma], 0.02% Tween-20 in PBS except for the GFP antibody, for which the PBS was replaced with TBS [Tris-Buffered Saline; 50 mM Tris, 150 mM NaCl, pH 7.5]).
Bellchambers H.M., Barratt K.S., Diamand K.E, & Arkell R.M. (2021). SUMOylation Potentiates ZIC Protein Activity to Influence Murine Neural Crest Cell Specification. International Journal of Molecular Sciences, 22(19), 10437.
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2

Immunofluorescence Staining of Cultured Cells

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Fixation of cultured cells grown on coverslips was with 4% paraformaldehyde in phosphate buffered saline (PBS) for 15 min followed by permeabilization with 0.5% Triton X-100 for 5 min for primary antibody staining. Alternatively, cells were fixed in cold methanol (−20°C) for 10 min. The following antibodies were used: rabbit monoclonal anti-SUN1 (Abcam), rabbit monoclonal anti-SUN2 (Abcam), mouse monoclonal anti-hnRNP F/H (ImmuQuest), mouse monoclonal anti-hnRNP K/J (ImmuQuest), rabbit polyclonal anti-Lamin B1 (Abcam), mouse monoclonal anti-NXF1 (Abcam), mouse monoclonal anti-RUVBL1 (Abcam), mouse monoclonal anti-Nuclear Pore Complex Proteins (mAb414) (Abcam), mouse monoclonal anti-Nup153 [QE5] (Abcam), rabbit polyclonal anti-GANP (Bethyl Laboratories) and mouse monoclonal anti-GAPDH−POD (Sigma). The secondary antibodies used were conjugated with Alexa Fluor 488 and Alexa Fluor 568 (Molecular Probes, Sigma). The samples were counterstained with DAPI (Sigma) for DNA and mounted in gelvatol. Samples were analyzed using confocal laser scanning microscopy (TCS-SP5, Leica) or Leica Structured Illumination (integration of the OptiGrid® module, Leica research microscopes and Leica MM AF software).
Li P, & Noegel A.A. (2015). Inner nuclear envelope protein SUN1 plays a prominent role in mammalian mRNA export. Nucleic Acids Research, 43(20), 9874-9888.
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3

Immunoblotting Antibody and Compound Protocol

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The following antibodies were purchased from the indicated supplied and used for immunoblotting: Rabbit monoclonal anti-HMGA1 (Sigma, cat. no. 129153), rabbit polyclonal anti-NAMPT (Bethyl Laboratories, cat. no. A300–372A), mouse monoclonal anti-β-Actin (Sigma, cat. no. 2532), ALDH1 (BD laboratories, cat. no. 611195), rabbit polyclonal anti-Lamin B1 (Abcam, cat. no. ab65986), cleaved PARP1 (Promega, cat. no. G7341), cleaved caspase 3 (Cell Signaling, cat. no. 9662). The following compounds were purchased from the indicated suppliers and used at the indicated concentrations for in vitro studies: cisplatin (Selleck, cat. no. S1166), 250 nM; carboplatin (Selleck, cat. no. S1215), 500 nM; FK866 (Millipore, cat. no. 48–190-82), 1 nM; GMX1778 (Selleck, cat. no. S8117), 0.5 nM; NMN (Sigma, cat. no. N3501), 500 μM; and doxycycline (Selleck, cat. no. S4163), 1 μg/ml.
Nacarelli T., Fukumoto T., Zundell J.A., Fatkhutdinov N., Jean S., Cadungog M.G., Borowsky M.E, & Zhang R. (2019). NAMPT inhibition suppresses cancer stem-like cells associated with therapy-induced senescence in ovarian cancer. Cancer research, 80(4), 890-900.
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4

Antibody Characterization for Western Blot and Immunofluorescence

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The following primary antibodies were from Cell Signaling Technology: Rabbit anti α-Tubilin (1:1000), mouse anti-p53 (1:1000) and rabbit anti-p53 (1:1000 for WB and 1:500 for immunofluorescence). Mouse monoclonal anti-Kindlin-2, clone 3A3 (1:2000 for WB and 1:500 for immunofluorescence) was from Millipore; rabbit polyclonal anti SerpinB2 (1:1000) and rabbit polyclonal anti Lamin B1 (1:3000) were from Abcam. Goat horseradish peroxidase-conjugated anti-mouse IgG (1:2000) and goat horseradish peroxidase-conjugated anti-rabbit IgG (1:2000) were from Calbiochem. Mouse monoclonal anti-Actin (1:5000) was from Sigma. Alexa Fluor Plus 594-conjugated anti-Rabbit IgG and Alexa Fluor Plus 488-conjugated anti-mouse IgG were from Invitrogen. Vecta-shield with 4′,6-diamidino-2-phenylindole (DAPI) was from Vector Laboratories. Gel electrophoresis reagents were from Bio-Rad.
Sossey-Alaoui K., Pluskota E., Szpak D, & Plow E.F. (2019). The Kindlin2-p53-SerpinB2 signaling axis is required for cellular senescence in breast cancer. Cell Death & Disease, 10(8), 539.
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5

Taxol/Paclitaxel Cell Imaging Protocol

Cited 1 time
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Taxol/paclitaxel were purchased from Sigma-Aldrich, Inc., and the dry powder was prepared for 100 μM stock solutions in DMSO. Tissue culture flasks (trade mark Falcon), tissue culture media, trypsin, and 100X antibiotic-antimycotic solution (Cellgro, Mediatech, Inc) were purchased from VWR Inc. (Springfield, NJ). Alexa Fluor 488 and 555 conjugated secondary antibodies were purchased from Life Sciences Inc. (Eugene, Oregon) for use in immunofluorescence microscopy. Primary antibodies: anti-Lamin A (1:400, H-102, rabbit polyclonal IgG); mouse monoclonal anti-Lamin B (1:300, sc373918); and goat polyclonal anti-Lamin B (1:400, sc6216) were from Santa Cruz Biotechnology Inc.; rabbit polyclonal anti-Lamin B1 (1:1000, ab16048) and mouse monoclonal anti- αTubulin (1:500, 66,031) were from Abcam and Proteintech, respectively. Mouse monoclonal anti-alpha-tubulin, were purchased from Sigma-Aldrich, Inc. (St. Louis, MO). More details of the reagents used are also reported previously [59 (link)–62 (link)].
Amaya C., Luo S., Baigorri J., Baucells R., Smith E.R, & Xu X.X. (2021). Exposure to low intensity ultrasound removes paclitaxel cytotoxicity in breast and ovarian cancer cells. BMC Cancer, 21, 981.
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6

Antibodies for Immunofluorescence and Analysis

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The following antibodies were used for immunofluorescence, FACS and immunoblot analysis: goat anti-Salmonella (CSA-1, Kirkegaard and Perry Laboratories), rabbit anti-DnaK [63 (link)], rat anti-HA (3F10, Roche), mouse anti-HA (HA11, Covance), mouse anti-FLAG (M2, Sigma-aldrich), rabbit anti-myc (Cell Signaling), mouse anti-β tubulin (Sigma-aldrich), rabbit polyclonal anti-GAPDH (Abcam), rabbit or goat anti-p65 (Santa Cruz), mouse anti- IĸBα (Cell Signaling), rabbit polyclonal anti-lamin B1 (Abcam), rabbit polyclonal anti-Xpo2 (CSE1L, Abcam), rabbit anti-Xpo1 (CRM1, Santa Cruz), rabbit anti-Xpo5 (Sigma-aldrich), rabbit anti-histone H3 (Abcam), rabbit anti-KPNA1 (Proteintech), mouse anti-p50 (Biolegend) and rabbit anti-STAT2 (Santa Cruz). Alexa Fluor 488-, 555- and 633- conjugated donkey anti-rat, anti-mouse, anti-goat and anti-rabbit antibodies were from Life technologies, UK.
Rolhion N., Furniss R.C., Grabe G., Ryan A., Liu M., Matthews S.A, & Holden D.W. (2016). Inhibition of Nuclear Transport of NF-ĸB p65 by the Salmonella Type III Secretion System Effector SpvD. PLoS Pathogens, 12(5), e1005653.
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7

HER2 Expression and Mechanism Validation

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To detect HER2 expression, HIBEC, RBE, HuccT1, CCLP1, HGBEC, SGC-996, NOZ, GBC-SD, and EH-GB1 cells were seeded in 6-well plates at the density of 4 × 105 per well. After 48 h, the total protein of each cell line was extracted. For mechanism validation, GBC-SD and EH-GB1 cells were seeded in 6-well plates at the density of 4 × 105 per well until adherent and replaced with different media. Each cell line was also divided into 8 groups and treated as previously described for the cell proliferation assay. After 48 h, total protein was extracted from each cell group. Then western blotting was performed according to the standard methods. The following antibodies, Rabbit monoclonal anti-HER2 (Abcam, Catalog no. ab134182, 1:1000 dilution), Mouse monoclonal anti-GAPDH (Abcam, Catalog no. ab8245, 1:5000 dilution), Rabbit monoclonal anti-NFκB p65 (Abcam, Catalog no. ab32536, 1:10,000 dilution), Rabbit monoclonal anti-NFκB p50 (Abcam, Catalog no. ab32360, 1:10,000 dilution), Mouse monoclonal anti-beta actin (Abcam, Catalog no. ab8226, Use a concentration of 1 µg/ml), and Rabbit polyclonal anti-Lamin B1 (Abcam, Nuclear Envelope Marker, Catalog no. ab16048; Use a concentration of 0.1 µg/ml) were obtained from Abcam. Supplementary Figures 37 and 38 depicts the source data underlying Supplementary Fig. 26 and Fig. 4b, respectively.
Juengpanich S., Li S., Yang T., Xie T., Chen J., Shan Y., Lee J., Lu Z., Chen T., Zhang B., Cao J., Hu J., Yu J., Wang Y., Topatana W., Gu Z., Cai X, & Chen M. (2023). Pre-activated nanoparticles with persistent luminescence for deep tumor photodynamic therapy in gallbladder cancer. Nature Communications, 14, 5699.
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8

Western Blot Analysis of p53 and Associated Proteins

Cited 2 times
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Protein lysates were prepared as described (Lener et al., 2009 (link)). Appropriate amounts of protein were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes as described (Greussing et al., 2013 (link)). Proteins of interest were detected by incubating the membranes with appropriate antibodies. Following primary antibodies were used: monoclonal mouse anti-p53 (#SC-126 Santa Cruz Biotechnology, USA), polyclonal rabbit anti-Lamin B1 (#ab16048 Abcam, United Kingdom), monoclonal rabbit anti-p21 waf1/cip1 (#2947S Cell signaling technology, USA), polyclonal rabbit anti-pp53 (Serin15) (#9284S Cell signaling technology, USA), and monoclonal mouse anti-GAPDH - glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (#SC-25778 Santa Cruz Biotechnology, USA). Appropriate polyclonal HRP-conjugated secondary antibodies were used (Dako Cytomation, Denmark). Detection was performed in a ChemiDoc Imaging system (Bio-Rad Laboratories, USA) using a chemiluminescence substrate kit (Merck Millipore, Germany). As positive controls protein lysates obtained from HDFs at passage 35 and Cisplatin-treated HDFs (33 μM) were used. Results were normalized to the loading control. Analysis of densitometry was performed in ImageJ software and the values were normalized to the loading control (GAPDH).
Martic I., Wedel S., Jansen-Dürr P, & Cavinato M. (2020). A new model to investigate UVB-induced cellular senescence and pigmentation in melanocytes. Mechanisms of ageing and development, 190, 111322.
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9

Western Blot Analysis of p53 and Associated Proteins

Cited 2 times
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Protein lysates were prepared as described (Lener et al., 2009 (link)). Appropriate amounts of protein were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes as described (Greussing et al., 2013 (link)). Proteins of interest were detected by incubating the membranes with appropriate antibodies. Following primary antibodies were used: monoclonal mouse anti-p53 (#SC-126 Santa Cruz Biotechnology, USA), polyclonal rabbit anti-Lamin B1 (#ab16048 Abcam, United Kingdom), monoclonal rabbit anti-p21 waf1/cip1 (#2947S Cell signaling technology, USA), polyclonal rabbit anti-pp53 (Serin15) (#9284S Cell signaling technology, USA), and monoclonal mouse anti-GAPDH - glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (#SC-25778 Santa Cruz Biotechnology, USA). Appropriate polyclonal HRP-conjugated secondary antibodies were used (Dako Cytomation, Denmark). Detection was performed in a ChemiDoc Imaging system (Bio-Rad Laboratories, USA) using a chemiluminescence substrate kit (Merck Millipore, Germany). As positive controls protein lysates obtained from HDFs at passage 35 and Cisplatin-treated HDFs (33 μM) were used. Results were normalized to the loading control. Analysis of densitometry was performed in ImageJ software and the values were normalized to the loading control (GAPDH).
Martic I., Wedel S., Jansen-Dürr P, & Cavinato M. (2020). A new model to investigate UVB-induced cellular senescence and pigmentation in melanocytes. Mechanisms of ageing and development, 190, 111322.
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